Efficient biosynthesis of exopolysaccharide in Candida glabrata by a fed-batch culture
Polysaccharides are important natural biomacromolecules. In particular, microbial exopolysaccharides have received much attention. They are produced by a variety of microorganisms, and they are widely used in the food, pharmaceutical, and chemical industries. The Candida glabrata mutant 4-C10, which...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.987796/full |
| _version_ | 1797999563619958784 |
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| author | Sha Xu Sha Xu Sha Xu Jinke Xu Jinke Xu Weizhu Zeng Weizhu Zeng Weizhu Zeng Xiaoyu Shan Xiaoyu Shan Jingwen Zhou Jingwen Zhou Jingwen Zhou Jingwen Zhou |
| author_facet | Sha Xu Sha Xu Sha Xu Jinke Xu Jinke Xu Weizhu Zeng Weizhu Zeng Weizhu Zeng Xiaoyu Shan Xiaoyu Shan Jingwen Zhou Jingwen Zhou Jingwen Zhou Jingwen Zhou |
| author_sort | Sha Xu |
| collection | DOAJ |
| description | Polysaccharides are important natural biomacromolecules. In particular, microbial exopolysaccharides have received much attention. They are produced by a variety of microorganisms, and they are widely used in the food, pharmaceutical, and chemical industries. The Candida glabrata mutant 4-C10, which has the capacity to produce exopolysaccharide, was previously obtained by random mutagenesis. In this study we aimed to further enhance exopolysaccharide production by systemic fermentation optimization. By single factor optimization and orthogonal design optimization in shaking flasks, an optimal fermentation medium composition was obtained. By optimizing agitation speed, aeration rate, and fed-batch fermentation mode, 118.6 g L−1 of exopolysaccharide was obtained by a constant rate feeding fermentation mode, with a glucose yield of 0.62 g g−1 and a productivity of 1.24 g L−1 h−1. Scaling up the established fermentation mode to a 15-L fermenter led to an exopolysaccharide yield of 113.8 g L−1, with a glucose yield of 0.60 g g−1 and a productivity of 1.29 g L−1 h−1. |
| first_indexed | 2024-04-11T11:06:41Z |
| format | Article |
| id | doaj.art-b7a86dcda12741f7b1015467ebff1aa4 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T11:06:41Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-b7a86dcda12741f7b1015467ebff1aa42022-12-22T04:28:19ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-09-011010.3389/fbioe.2022.987796987796Efficient biosynthesis of exopolysaccharide in Candida glabrata by a fed-batch cultureSha Xu0Sha Xu1Sha Xu2Jinke Xu3Jinke Xu4Weizhu Zeng5Weizhu Zeng6Weizhu Zeng7Xiaoyu Shan8Xiaoyu Shan9Jingwen Zhou10Jingwen Zhou11Jingwen Zhou12Jingwen Zhou13National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, ChinaSchool of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, ChinaJiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi, ChinaSchool of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, ChinaScience Center for Future Foods, Jiangnan University, Wuxi, ChinaSchool of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, ChinaJiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi, ChinaScience Center for Future Foods, Jiangnan University, Wuxi, ChinaNational Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, ChinaScience Center for Future Foods, Jiangnan University, Wuxi, ChinaNational Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, ChinaSchool of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, ChinaJiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi, ChinaScience Center for Future Foods, Jiangnan University, Wuxi, ChinaPolysaccharides are important natural biomacromolecules. In particular, microbial exopolysaccharides have received much attention. They are produced by a variety of microorganisms, and they are widely used in the food, pharmaceutical, and chemical industries. The Candida glabrata mutant 4-C10, which has the capacity to produce exopolysaccharide, was previously obtained by random mutagenesis. In this study we aimed to further enhance exopolysaccharide production by systemic fermentation optimization. By single factor optimization and orthogonal design optimization in shaking flasks, an optimal fermentation medium composition was obtained. By optimizing agitation speed, aeration rate, and fed-batch fermentation mode, 118.6 g L−1 of exopolysaccharide was obtained by a constant rate feeding fermentation mode, with a glucose yield of 0.62 g g−1 and a productivity of 1.24 g L−1 h−1. Scaling up the established fermentation mode to a 15-L fermenter led to an exopolysaccharide yield of 113.8 g L−1, with a glucose yield of 0.60 g g−1 and a productivity of 1.29 g L−1 h−1.https://www.frontiersin.org/articles/10.3389/fbioe.2022.987796/fullCandida glabrataexopolysaccharidemedium compositionfed-batch fermentationoverproduction |
| spellingShingle | Sha Xu Sha Xu Sha Xu Jinke Xu Jinke Xu Weizhu Zeng Weizhu Zeng Weizhu Zeng Xiaoyu Shan Xiaoyu Shan Jingwen Zhou Jingwen Zhou Jingwen Zhou Jingwen Zhou Efficient biosynthesis of exopolysaccharide in Candida glabrata by a fed-batch culture Frontiers in Bioengineering and Biotechnology Candida glabrata exopolysaccharide medium composition fed-batch fermentation overproduction |
| title | Efficient biosynthesis of exopolysaccharide in Candida glabrata by a fed-batch culture |
| title_full | Efficient biosynthesis of exopolysaccharide in Candida glabrata by a fed-batch culture |
| title_fullStr | Efficient biosynthesis of exopolysaccharide in Candida glabrata by a fed-batch culture |
| title_full_unstemmed | Efficient biosynthesis of exopolysaccharide in Candida glabrata by a fed-batch culture |
| title_short | Efficient biosynthesis of exopolysaccharide in Candida glabrata by a fed-batch culture |
| title_sort | efficient biosynthesis of exopolysaccharide in candida glabrata by a fed batch culture |
| topic | Candida glabrata exopolysaccharide medium composition fed-batch fermentation overproduction |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.987796/full |
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