Confirmation of ‘Pollen- and Seed-Specific Gene Deletor’ System Efficiency for Transgene Excision from Transgenic <i>Nicotiana tabacum</i> under Field Conditions
The commercial application of genetically modified plants has been seriously impeded by public concern surrounding the potential risks posed by such plants to the ecosystem and human health. Previously, we have developed a ‘pollen- and seed-specific Gene Deletor’ system that automatically excised al...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/2/1160 |
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| author | Zhenzhen Duan Mingyang He Sehrish Akbar Degang Zhao Muqing Zhang Yi Li Wei Yao |
| author_facet | Zhenzhen Duan Mingyang He Sehrish Akbar Degang Zhao Muqing Zhang Yi Li Wei Yao |
| author_sort | Zhenzhen Duan |
| collection | DOAJ |
| description | The commercial application of genetically modified plants has been seriously impeded by public concern surrounding the potential risks posed by such plants to the ecosystem and human health. Previously, we have developed a ‘pollen- and seed-specific Gene Deletor’ system that automatically excised all transgenes from the pollen and seeds of greenhouse-grown transgenic <i>Nicotiana tabacum</i>. In this study, we conducted seven field experiments over three consecutive years to evaluate the stability of transgene excision under field conditions. Our results showed that transgenes were stably excised from transgenic <i>Nicotiana tabacum</i> under field conditions with 100% efficiency. The stability of transgene excision was confirmed based on PCR, as well as the GUS staining patterns of various organs (roots, leaves, petiole, stem, flower, fruit, and seeds) from transgenic <i>N. tabacum</i>. In six transgenic lines (D4, D10, D31, D56, and D43), the transgenes were stably deleted in the T0 and T1 generations. Thus, the ‘Gene Deletor’ system is an efficient and reliable method to reduce pollen- and seed-mediated unintentional gene flow. This system might help to alleviate the food safety concerns associated with transgenic crops. |
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| id | doaj.art-b7a981a238a94a2cabd146851669c5a2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:25:07Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b7a981a238a94a2cabd146851669c5a22023-11-30T22:36:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242116010.3390/ijms24021160Confirmation of ‘Pollen- and Seed-Specific Gene Deletor’ System Efficiency for Transgene Excision from Transgenic <i>Nicotiana tabacum</i> under Field ConditionsZhenzhen Duan0Mingyang He1Sehrish Akbar2Degang Zhao3Muqing Zhang4Yi Li5Wei Yao6State Key Laboratory for Conservation and Utilization of Subtropical Agro-Biological Resources & Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, USAState Key Laboratory for Conservation and Utilization of Subtropical Agro-Biological Resources & Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, USAState Key Laboratory for Conservation and Utilization of Subtropical Agro-Biological Resources & Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, USAState Key Laboratory for Conservation and Utilization of Subtropical Agro-Biological Resources & Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaThe commercial application of genetically modified plants has been seriously impeded by public concern surrounding the potential risks posed by such plants to the ecosystem and human health. Previously, we have developed a ‘pollen- and seed-specific Gene Deletor’ system that automatically excised all transgenes from the pollen and seeds of greenhouse-grown transgenic <i>Nicotiana tabacum</i>. In this study, we conducted seven field experiments over three consecutive years to evaluate the stability of transgene excision under field conditions. Our results showed that transgenes were stably excised from transgenic <i>Nicotiana tabacum</i> under field conditions with 100% efficiency. The stability of transgene excision was confirmed based on PCR, as well as the GUS staining patterns of various organs (roots, leaves, petiole, stem, flower, fruit, and seeds) from transgenic <i>N. tabacum</i>. In six transgenic lines (D4, D10, D31, D56, and D43), the transgenes were stably deleted in the T0 and T1 generations. Thus, the ‘Gene Deletor’ system is an efficient and reliable method to reduce pollen- and seed-mediated unintentional gene flow. This system might help to alleviate the food safety concerns associated with transgenic crops.https://www.mdpi.com/1422-0067/24/2/1160auto transgene excisionFLP recombinasefield evaluationtransgene flowGene Deletor |
| spellingShingle | Zhenzhen Duan Mingyang He Sehrish Akbar Degang Zhao Muqing Zhang Yi Li Wei Yao Confirmation of ‘Pollen- and Seed-Specific Gene Deletor’ System Efficiency for Transgene Excision from Transgenic <i>Nicotiana tabacum</i> under Field Conditions International Journal of Molecular Sciences auto transgene excision FLP recombinase field evaluation transgene flow Gene Deletor |
| title | Confirmation of ‘Pollen- and Seed-Specific Gene Deletor’ System Efficiency for Transgene Excision from Transgenic <i>Nicotiana tabacum</i> under Field Conditions |
| title_full | Confirmation of ‘Pollen- and Seed-Specific Gene Deletor’ System Efficiency for Transgene Excision from Transgenic <i>Nicotiana tabacum</i> under Field Conditions |
| title_fullStr | Confirmation of ‘Pollen- and Seed-Specific Gene Deletor’ System Efficiency for Transgene Excision from Transgenic <i>Nicotiana tabacum</i> under Field Conditions |
| title_full_unstemmed | Confirmation of ‘Pollen- and Seed-Specific Gene Deletor’ System Efficiency for Transgene Excision from Transgenic <i>Nicotiana tabacum</i> under Field Conditions |
| title_short | Confirmation of ‘Pollen- and Seed-Specific Gene Deletor’ System Efficiency for Transgene Excision from Transgenic <i>Nicotiana tabacum</i> under Field Conditions |
| title_sort | confirmation of pollen and seed specific gene deletor system efficiency for transgene excision from transgenic i nicotiana tabacum i under field conditions |
| topic | auto transgene excision FLP recombinase field evaluation transgene flow Gene Deletor |
| url | https://www.mdpi.com/1422-0067/24/2/1160 |
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