Enhancing zinc levels in Solanum lycopersicum L. through biofortification with plant growth-promoting Pseudomonas spp. isolated from cow dung
Zinc is a vital micronutrient for all life forms, and Zn-solubilizing bacteria (ZSB) present in the soil convert in organic zinc into forms available for plants. This study assessed ZSB isolated from cow dung for their plant growth-promoting (PGP) characteristics and potential to enhance tomato plan...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2023-06-01
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| Series: | BioTechnologia |
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| Online Access: | https://www.termedia.pl/Enhancing-zinc-levels-in-Solanum-lycopersicum-L-r-nthrough-biofortification-with-plant-growth-promoting-r-nPseudomonas-spp-isolated-from-cow-dung,85,50642,1,1.html |
| _version_ | 1797692415198363648 |
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| author | Arun Karnwal |
| author_facet | Arun Karnwal |
| author_sort | Arun Karnwal |
| collection | DOAJ |
| description | Zinc is a vital micronutrient for all life forms, and Zn-solubilizing bacteria (ZSB) present in the soil convert in organic zinc into forms available for plants. This study assessed ZSB isolated from cow dung for their plant growth-promoting (PGP) characteristics and potential to enhance tomato plant growth. The experiment assayed a total of 30 bacteria from cow dung for Zn-solubilization using insoluble ZnO and ZnCO 3 . Atomic absorption spectro¬scopy quantitatively evaluated Zn-solubilization, and the isolates were further studied for Zn-solubilization and plant growth in Solanum lycopersicum . The CDS7 and CDS27 isolates were the most significant Zn-solubilizing strains. CDS7 exhibited increased ZnO solubility (32.1 mg/l) compared to CDS21 (23.7 mg/l). PGP trait quantitative results revealed that the CDS7 and CDS21 bacterial strains solubilized insoluble phosphate (287.2 and 217.7 μg/ml, respectively) and produced indole acetic acid (22.1 and 14.8 μg/ml, respectively). Based on 16S rRNA gene sequencing, CDS7 and CDS21 were identified as Pseudomonas kilonensis and Pseudomonas chlororaphis , and 16S rDNA sequences were submitted to the GenBank database. Furthermore, ZSB strains were administered to tomato seeds under a pot study. The treatments with CDS7 inoculant and a consortium of both isolates were reported with maximum plant development (stem length 63.16 and 59.89 cm, respectively) and zinc content (3.13 and 2.36 mg/100 g, respectively) in tomato fruit compared to the control. In conclusion, microorganisms isolated from cow dung with PGP activity can improve Zn bioavailability and plant growth sustainably. They can be used as biofertilizers in agricultural fields to improve plant growth and production. |
| first_indexed | 2024-03-12T02:27:17Z |
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| id | doaj.art-b7abaa2e49b545d2bf311781c80b7e95 |
| institution | Directory Open Access Journal |
| issn | 0860-7796 2353-9461 |
| language | English |
| last_indexed | 2024-03-12T02:27:17Z |
| publishDate | 2023-06-01 |
| publisher | Termedia Publishing House |
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| series | BioTechnologia |
| spelling | doaj.art-b7abaa2e49b545d2bf311781c80b7e952023-09-05T11:45:55ZengTermedia Publishing HouseBioTechnologia0860-77962353-94612023-06-01104215716910.5114/bta.2023.12720550642Enhancing zinc levels in Solanum lycopersicum L. through biofortification with plant growth-promoting Pseudomonas spp. isolated from cow dungArun KarnwalZinc is a vital micronutrient for all life forms, and Zn-solubilizing bacteria (ZSB) present in the soil convert in organic zinc into forms available for plants. This study assessed ZSB isolated from cow dung for their plant growth-promoting (PGP) characteristics and potential to enhance tomato plant growth. The experiment assayed a total of 30 bacteria from cow dung for Zn-solubilization using insoluble ZnO and ZnCO 3 . Atomic absorption spectro¬scopy quantitatively evaluated Zn-solubilization, and the isolates were further studied for Zn-solubilization and plant growth in Solanum lycopersicum . The CDS7 and CDS27 isolates were the most significant Zn-solubilizing strains. CDS7 exhibited increased ZnO solubility (32.1 mg/l) compared to CDS21 (23.7 mg/l). PGP trait quantitative results revealed that the CDS7 and CDS21 bacterial strains solubilized insoluble phosphate (287.2 and 217.7 μg/ml, respectively) and produced indole acetic acid (22.1 and 14.8 μg/ml, respectively). Based on 16S rRNA gene sequencing, CDS7 and CDS21 were identified as Pseudomonas kilonensis and Pseudomonas chlororaphis , and 16S rDNA sequences were submitted to the GenBank database. Furthermore, ZSB strains were administered to tomato seeds under a pot study. The treatments with CDS7 inoculant and a consortium of both isolates were reported with maximum plant development (stem length 63.16 and 59.89 cm, respectively) and zinc content (3.13 and 2.36 mg/100 g, respectively) in tomato fruit compared to the control. In conclusion, microorganisms isolated from cow dung with PGP activity can improve Zn bioavailability and plant growth sustainably. They can be used as biofertilizers in agricultural fields to improve plant growth and production.https://www.termedia.pl/Enhancing-zinc-levels-in-Solanum-lycopersicum-L-r-nthrough-biofortification-with-plant-growth-promoting-r-nPseudomonas-spp-isolated-from-cow-dung,85,50642,1,1.htmlbiofortification plant growth promotion pseudomonas solanum lycopersicum zinc |
| spellingShingle | Arun Karnwal Enhancing zinc levels in Solanum lycopersicum L. through biofortification with plant growth-promoting Pseudomonas spp. isolated from cow dung BioTechnologia biofortification plant growth promotion pseudomonas solanum lycopersicum zinc |
| title | Enhancing zinc levels in Solanum lycopersicum L.
through biofortification with plant growth-promoting
Pseudomonas spp. isolated from cow dung |
| title_full | Enhancing zinc levels in Solanum lycopersicum L.
through biofortification with plant growth-promoting
Pseudomonas spp. isolated from cow dung |
| title_fullStr | Enhancing zinc levels in Solanum lycopersicum L.
through biofortification with plant growth-promoting
Pseudomonas spp. isolated from cow dung |
| title_full_unstemmed | Enhancing zinc levels in Solanum lycopersicum L.
through biofortification with plant growth-promoting
Pseudomonas spp. isolated from cow dung |
| title_short | Enhancing zinc levels in Solanum lycopersicum L.
through biofortification with plant growth-promoting
Pseudomonas spp. isolated from cow dung |
| title_sort | enhancing zinc levels in solanum lycopersicum l through biofortification with plant growth promoting pseudomonas spp isolated from cow dung |
| topic | biofortification plant growth promotion pseudomonas solanum lycopersicum zinc |
| url | https://www.termedia.pl/Enhancing-zinc-levels-in-Solanum-lycopersicum-L-r-nthrough-biofortification-with-plant-growth-promoting-r-nPseudomonas-spp-isolated-from-cow-dung,85,50642,1,1.html |
| work_keys_str_mv | AT arunkarnwal enhancingzinclevelsinsolanumlycopersicumlthroughbiofortificationwithplantgrowthpromotingpseudomonassppisolatedfromcowdung |