An Artificial Insulin Receptor that Self-assembles and Works on a Gold Surface
A novel artificial insulin receptor was developed, that can self-assemble on a gold surface and alter its structure in response to insulin recognition via partial domains of the intrinsic insulin receptor. The candidates for the artificial insulin receptor were designed by fusing the αCT segment and...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The Electrochemical Society of Japan
2024-02-01
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| Series: | Electrochemistry |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/electrochemistry/92/2/92_23-68095/_html/-char/en |
| _version_ | 1827339068450537472 |
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| author | Yusuke MURAI Hiroya SATO Hayate INOUE Eiji ARITA Yoshiaki YAGUCHI Takenori ISHIDA Takeshi IKEDA Ryuichi HIROTA Akio KURODA Hisakage FUNABASHI |
| author_facet | Yusuke MURAI Hiroya SATO Hayate INOUE Eiji ARITA Yoshiaki YAGUCHI Takenori ISHIDA Takeshi IKEDA Ryuichi HIROTA Akio KURODA Hisakage FUNABASHI |
| author_sort | Yusuke MURAI |
| collection | DOAJ |
| description | A novel artificial insulin receptor was developed, that can self-assemble on a gold surface and alter its structure in response to insulin recognition via partial domains of the intrinsic insulin receptor. The candidates for the artificial insulin receptor were designed by fusing the αCT segment and L1CR domain of the insulin receptor with a gold-binding peptide to have self-assembling abilities on a gold surface. The proteins were termed 3GαL and αL3G, based on the order of these domains, and expressed in mammalian cells. A quartz crystal microbalance technique confirmed the ability of both proteins to self-assemble on the gold surface. Electrochemical impedance spectroscopy measurements using gold electrodes modified with these proteins revealed that 3GαL altered its structure in response to insulin recognition, even on a gold surface, confirming that it works as an artificial insulin receptor that self-assembles on a gold surface. We expect that 3GαL will contribute to the development of various biosensors that utilize gold surfaces as insulin-recognition elements. |
| first_indexed | 2024-03-07T19:45:45Z |
| format | Article |
| id | doaj.art-b7afe706a6a24c389dbd298170d11e67 |
| institution | Directory Open Access Journal |
| issn | 2186-2451 |
| language | English |
| last_indexed | 2024-03-07T19:45:45Z |
| publishDate | 2024-02-01 |
| publisher | The Electrochemical Society of Japan |
| record_format | Article |
| series | Electrochemistry |
| spelling | doaj.art-b7afe706a6a24c389dbd298170d11e672024-02-29T02:10:51ZengThe Electrochemical Society of JapanElectrochemistry2186-24512024-02-0192202200602200610.5796/electrochemistry.23-68095electrochemistryAn Artificial Insulin Receptor that Self-assembles and Works on a Gold SurfaceYusuke MURAI0Hiroya SATO1Hayate INOUE2Eiji ARITA3Yoshiaki YAGUCHI4Takenori ISHIDA5https://orcid.org/0000-0002-6705-915XTakeshi IKEDA6https://orcid.org/0000-0001-7159-5660Ryuichi HIROTA7https://orcid.org/0000-0003-3959-5892Akio KURODA8https://orcid.org/0009-0000-3156-5387Hisakage FUNABASHI9https://orcid.org/0000-0003-2330-1503Division of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima UniversityR&D Department, Life Care Solutions Division, Medical Care Solutions Company, Terumo Corporation R&D CenterDivision of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima UniversityR&D Department, Life Care Solutions Division, Medical Care Solutions Company, Terumo Corporation R&D CenterR&D Department, Life Care Solutions Division, Medical Care Solutions Company, Terumo Corporation R&D CenterDivision of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima UniversityDivision of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima UniversityDivision of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima UniversityDivision of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima UniversityDivision of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima UniversityA novel artificial insulin receptor was developed, that can self-assemble on a gold surface and alter its structure in response to insulin recognition via partial domains of the intrinsic insulin receptor. The candidates for the artificial insulin receptor were designed by fusing the αCT segment and L1CR domain of the insulin receptor with a gold-binding peptide to have self-assembling abilities on a gold surface. The proteins were termed 3GαL and αL3G, based on the order of these domains, and expressed in mammalian cells. A quartz crystal microbalance technique confirmed the ability of both proteins to self-assemble on the gold surface. Electrochemical impedance spectroscopy measurements using gold electrodes modified with these proteins revealed that 3GαL altered its structure in response to insulin recognition, even on a gold surface, confirming that it works as an artificial insulin receptor that self-assembles on a gold surface. We expect that 3GαL will contribute to the development of various biosensors that utilize gold surfaces as insulin-recognition elements.https://www.jstage.jst.go.jp/article/electrochemistry/92/2/92_23-68095/_html/-char/enartificial insulin receptorgold binding peptideinsulin sensor |
| spellingShingle | Yusuke MURAI Hiroya SATO Hayate INOUE Eiji ARITA Yoshiaki YAGUCHI Takenori ISHIDA Takeshi IKEDA Ryuichi HIROTA Akio KURODA Hisakage FUNABASHI An Artificial Insulin Receptor that Self-assembles and Works on a Gold Surface Electrochemistry artificial insulin receptor gold binding peptide insulin sensor |
| title | An Artificial Insulin Receptor that Self-assembles and Works on a Gold Surface |
| title_full | An Artificial Insulin Receptor that Self-assembles and Works on a Gold Surface |
| title_fullStr | An Artificial Insulin Receptor that Self-assembles and Works on a Gold Surface |
| title_full_unstemmed | An Artificial Insulin Receptor that Self-assembles and Works on a Gold Surface |
| title_short | An Artificial Insulin Receptor that Self-assembles and Works on a Gold Surface |
| title_sort | artificial insulin receptor that self assembles and works on a gold surface |
| topic | artificial insulin receptor gold binding peptide insulin sensor |
| url | https://www.jstage.jst.go.jp/article/electrochemistry/92/2/92_23-68095/_html/-char/en |
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