Effects of green synthesised silver nanoparticles (ST06-AgNPs) using curcumin derivative (ST06) on human cervical cancer cells (HeLa) in vitro and EAC tumor bearing mice models
Kalaimathi Murugesan,*,1 Jinsha Koroth,*,1,2 Padma Priya Srinivasan,1 Amrita Singh,3 Sanjana Mukundan,1 Subhas S Karki,4 Bibha Choudhary,1 Chhitar M Gupta11Institute of Bioinformatics and Applied Biotechnology (IBAB), Bangalore, India; 2Department of Pharmaceutical Chemistry, Manipal Academ...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-07-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/effects-of-green-synthesised-silver-nanoparticles-st06-agnps-using-cur-peer-reviewed-article-IJN |
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| author | Murugesan K Koroth J Srinivasan PP Singh A Mukundan S Karki SS Choudhary B Gupta CM |
| author_facet | Murugesan K Koroth J Srinivasan PP Singh A Mukundan S Karki SS Choudhary B Gupta CM |
| author_sort | Murugesan K |
| collection | DOAJ |
| description | Kalaimathi Murugesan,*,1 Jinsha Koroth,*,1,2 Padma Priya Srinivasan,1 Amrita Singh,3 Sanjana Mukundan,1 Subhas S Karki,4 Bibha Choudhary,1 Chhitar M Gupta11Institute of Bioinformatics and Applied Biotechnology (IBAB), Bangalore, India; 2Department of Pharmaceutical Chemistry, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India; 3Water Analysis Laboratory, Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research, Lucknow, India; 4KLE Academy of Higher Education & Research, KLE College of Pharmacy, Bangalore, KN, India*These authors contributed equally to this workBackground: In recent years, green synthesized silver nanoparticles have been increasingly investigated for their anti-cancer potential. In the present study, we aimed at the biosynthesis of silver nanoparticles (AgNPs) using a curcumin derivative, ST06. Although, the individual efficacies of silver nanoparticles or curcumin derivatives have been studied previously, the synergistic cytotoxic effects of curcumin derivative and silver nanoparticles in a single nanoparticulate formulation have not been studied earlier specifically on animal models. This makes this study novel compared to the earlier synthesized curcumin derivative or silver nanoparticles studies. The aim of the study was to synthesize ST06 coated silver nanoparticles (ST06-AgNPs) using ST06 as both reducing and coating agent.Methods: The synthesized nanoparticles AgNPs and ST06-AgNPs were characterised for the particle size distribution, morphology, optical properties and surface charge by using UV-visible spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM). Elemental composition and structural properties were studied by energy dispersive X-ray spectroscopy (EDX) and X-ray diffraction spectroscopy (XRD). The presence of ST06 as capping agent was demonstrated by Fourier transform infrared spectroscopy (FTIR).Results: The synthesized nanoparticles (ST06-AgNPs) were spherical and had a size distribution in the range of 50–100 nm. UV-Vis spectroscopy displayed a specific silver plasmon peak at 410 nm. The in vitro cytotoxicity effects of ST06 and ST06-AgNPs, as assessed by MTT assay, showed significant growth inhibition of human cervical cancer cell line (HeLa). In addition, studies carried out in EAC tumor-induced mouse model (Ehrlich Ascites carcinoma) using ST06-AgNPs, revealed that treatment of the animals with these nanoparticles resulted in a significant reduction in the tumor growth, compared to the control group animals.Conclusion: In conclusion, green synthesized ST06-AgNPs exhibited superior anti-tumor efficacy than the free ST06 or AgNPs with no acute toxicity under both in vitro and in vivo conditions. The tumor suppression is associated with the intrinsic apoptotic pathway. Together, the results of this study suggest that ST06-AgNPs could be considered as a potential option for the treatment of solid tumors.Keywords: silver nanoparticles, anticancer, Ehrlich Ascites carcinoma, apoptosis |
| first_indexed | 2024-12-21T00:45:52Z |
| format | Article |
| id | doaj.art-b7b1027274e54c3d931b34a6093eb6d7 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-21T00:45:52Z |
| publishDate | 2019-07-01 |
| publisher | Dove Medical Press |
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| series | International Journal of Nanomedicine |
| spelling | doaj.art-b7b1027274e54c3d931b34a6093eb6d72022-12-21T19:21:31ZengDove Medical PressInternational Journal of Nanomedicine1178-20132019-07-01Volume 145257527047150Effects of green synthesised silver nanoparticles (ST06-AgNPs) using curcumin derivative (ST06) on human cervical cancer cells (HeLa) in vitro and EAC tumor bearing mice modelsMurugesan KKoroth JSrinivasan PPSingh AMukundan SKarki SSChoudhary BGupta CMKalaimathi Murugesan,*,1 Jinsha Koroth,*,1,2 Padma Priya Srinivasan,1 Amrita Singh,3 Sanjana Mukundan,1 Subhas S Karki,4 Bibha Choudhary,1 Chhitar M Gupta11Institute of Bioinformatics and Applied Biotechnology (IBAB), Bangalore, India; 2Department of Pharmaceutical Chemistry, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India; 3Water Analysis Laboratory, Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research, Lucknow, India; 4KLE Academy of Higher Education & Research, KLE College of Pharmacy, Bangalore, KN, India*These authors contributed equally to this workBackground: In recent years, green synthesized silver nanoparticles have been increasingly investigated for their anti-cancer potential. In the present study, we aimed at the biosynthesis of silver nanoparticles (AgNPs) using a curcumin derivative, ST06. Although, the individual efficacies of silver nanoparticles or curcumin derivatives have been studied previously, the synergistic cytotoxic effects of curcumin derivative and silver nanoparticles in a single nanoparticulate formulation have not been studied earlier specifically on animal models. This makes this study novel compared to the earlier synthesized curcumin derivative or silver nanoparticles studies. The aim of the study was to synthesize ST06 coated silver nanoparticles (ST06-AgNPs) using ST06 as both reducing and coating agent.Methods: The synthesized nanoparticles AgNPs and ST06-AgNPs were characterised for the particle size distribution, morphology, optical properties and surface charge by using UV-visible spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM). Elemental composition and structural properties were studied by energy dispersive X-ray spectroscopy (EDX) and X-ray diffraction spectroscopy (XRD). The presence of ST06 as capping agent was demonstrated by Fourier transform infrared spectroscopy (FTIR).Results: The synthesized nanoparticles (ST06-AgNPs) were spherical and had a size distribution in the range of 50–100 nm. UV-Vis spectroscopy displayed a specific silver plasmon peak at 410 nm. The in vitro cytotoxicity effects of ST06 and ST06-AgNPs, as assessed by MTT assay, showed significant growth inhibition of human cervical cancer cell line (HeLa). In addition, studies carried out in EAC tumor-induced mouse model (Ehrlich Ascites carcinoma) using ST06-AgNPs, revealed that treatment of the animals with these nanoparticles resulted in a significant reduction in the tumor growth, compared to the control group animals.Conclusion: In conclusion, green synthesized ST06-AgNPs exhibited superior anti-tumor efficacy than the free ST06 or AgNPs with no acute toxicity under both in vitro and in vivo conditions. The tumor suppression is associated with the intrinsic apoptotic pathway. Together, the results of this study suggest that ST06-AgNPs could be considered as a potential option for the treatment of solid tumors.Keywords: silver nanoparticles, anticancer, Ehrlich Ascites carcinoma, apoptosishttps://www.dovepress.com/effects-of-green-synthesised-silver-nanoparticles-st06-agnps-using-cur-peer-reviewed-article-IJNsilver nanoparticlesanticancerEhrlich Ascites carcinomaapoptosis |
| spellingShingle | Murugesan K Koroth J Srinivasan PP Singh A Mukundan S Karki SS Choudhary B Gupta CM Effects of green synthesised silver nanoparticles (ST06-AgNPs) using curcumin derivative (ST06) on human cervical cancer cells (HeLa) in vitro and EAC tumor bearing mice models International Journal of Nanomedicine silver nanoparticles anticancer Ehrlich Ascites carcinoma apoptosis |
| title | Effects of green synthesised silver nanoparticles (ST06-AgNPs) using curcumin derivative (ST06) on human cervical cancer cells (HeLa) in vitro and EAC tumor bearing mice models |
| title_full | Effects of green synthesised silver nanoparticles (ST06-AgNPs) using curcumin derivative (ST06) on human cervical cancer cells (HeLa) in vitro and EAC tumor bearing mice models |
| title_fullStr | Effects of green synthesised silver nanoparticles (ST06-AgNPs) using curcumin derivative (ST06) on human cervical cancer cells (HeLa) in vitro and EAC tumor bearing mice models |
| title_full_unstemmed | Effects of green synthesised silver nanoparticles (ST06-AgNPs) using curcumin derivative (ST06) on human cervical cancer cells (HeLa) in vitro and EAC tumor bearing mice models |
| title_short | Effects of green synthesised silver nanoparticles (ST06-AgNPs) using curcumin derivative (ST06) on human cervical cancer cells (HeLa) in vitro and EAC tumor bearing mice models |
| title_sort | effects of green synthesised silver nanoparticles st06 agnps using curcumin derivative st06 on human cervical cancer cells hela in vitro and eac tumor bearing mice models |
| topic | silver nanoparticles anticancer Ehrlich Ascites carcinoma apoptosis |
| url | https://www.dovepress.com/effects-of-green-synthesised-silver-nanoparticles-st06-agnps-using-cur-peer-reviewed-article-IJN |
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