NOD2/CARD15 polymorphisms (P268S, IVS8+158, G908R, L1007fs, R702W) among Kuwaiti patients with Crohn's disease: A case-control study
Background: Nucleotide-binding oligomerization domain-containing two (NOD2/CARD15) gene polymorphisms are implicated in the pathogenesis of Crohn's disease (CD). Aim: To describe the allelic frequency of NOD2/CARD15 gene variants among Kuwaiti patients with CD and investigate potential genotype...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | The Saudi Journal of Gastroenterology |
| Subjects: | |
| Online Access: | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2021;volume=27;issue=4;spage=249;epage=256;aulast=Abdelnaby |
| _version_ | 1819019877749358592 |
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| author | Hassan Abdelnaby Ndeye Coumba Ndiaye Ferdinando D'Amico Ahmed Mahmoud Fouad Sameh Hassan Alaa Elshafey Wafaa Al Hashash Mohammed Faisal Yousef Alshamali Talal Al-Taweel Laurent Peyrin-Biroulet |
| author_facet | Hassan Abdelnaby Ndeye Coumba Ndiaye Ferdinando D'Amico Ahmed Mahmoud Fouad Sameh Hassan Alaa Elshafey Wafaa Al Hashash Mohammed Faisal Yousef Alshamali Talal Al-Taweel Laurent Peyrin-Biroulet |
| author_sort | Hassan Abdelnaby |
| collection | DOAJ |
| description | Background: Nucleotide-binding oligomerization domain-containing two (NOD2/CARD15) gene polymorphisms are implicated in the pathogenesis of Crohn's disease (CD).
Aim: To describe the allelic frequency of NOD2/CARD15 gene variants among Kuwaiti patients with CD and investigate potential genotype/phenotype associations.
Methods: Adult Kuwaiti citizens with an established diagnosis of CD and healthy controls were enrolled from October 2018 to May 2020. Three common NOD2/CARD15 polymorphisms (R702W, G908R, and L1007fs) and P268S and IVS8+158 polymorphisms were screened by polymerase chain reaction/restriction analysis length polymorphism (PCR/RFLP).
Results: Ninety adult Kuwaiti patients with CD and 210 healthy subjects (as controls) were recruited. P268S, IVS8+158, G908R, and R702W minor alleles were identified in 38.9%, 21.1%, 12.2%, and 4.4% of CD patients, respectively. NOD2/CARD15 polymorphisms coexisted in 35 healthy controls (16.7%) and 21 CD patients (23.3%). Individuals with either a single or multiple polymorphism were approximately two times more likely to have CD than those with no polymorphism. Patients with multiple polymorphisms had significantly more stricturing and penetrating disease.
Conclusion: NOD2/CARD15 gene polymorphisms were significantly associated with an increased risk of disease and aggressive phenotypes among the Kuwaiti CD population. |
| first_indexed | 2024-12-21T03:42:18Z |
| format | Article |
| id | doaj.art-b7b4204a03d2490b88314007fa64641a |
| institution | Directory Open Access Journal |
| issn | 1319-3767 1998-4049 |
| language | English |
| last_indexed | 2024-12-21T03:42:18Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | The Saudi Journal of Gastroenterology |
| spelling | doaj.art-b7b4204a03d2490b88314007fa64641a2022-12-21T19:17:12ZengWolters Kluwer Medknow PublicationsThe Saudi Journal of Gastroenterology1319-37671998-40492021-01-0127424925610.4103/sjg.sjg_613_20NOD2/CARD15 polymorphisms (P268S, IVS8+158, G908R, L1007fs, R702W) among Kuwaiti patients with Crohn's disease: A case-control studyHassan AbdelnabyNdeye Coumba NdiayeFerdinando D'AmicoAhmed Mahmoud FouadSameh HassanAlaa ElshafeyWafaa Al HashashMohammed FaisalYousef AlshamaliTalal Al-TaweelLaurent Peyrin-BirouletBackground: Nucleotide-binding oligomerization domain-containing two (NOD2/CARD15) gene polymorphisms are implicated in the pathogenesis of Crohn's disease (CD). Aim: To describe the allelic frequency of NOD2/CARD15 gene variants among Kuwaiti patients with CD and investigate potential genotype/phenotype associations. Methods: Adult Kuwaiti citizens with an established diagnosis of CD and healthy controls were enrolled from October 2018 to May 2020. Three common NOD2/CARD15 polymorphisms (R702W, G908R, and L1007fs) and P268S and IVS8+158 polymorphisms were screened by polymerase chain reaction/restriction analysis length polymorphism (PCR/RFLP). Results: Ninety adult Kuwaiti patients with CD and 210 healthy subjects (as controls) were recruited. P268S, IVS8+158, G908R, and R702W minor alleles were identified in 38.9%, 21.1%, 12.2%, and 4.4% of CD patients, respectively. NOD2/CARD15 polymorphisms coexisted in 35 healthy controls (16.7%) and 21 CD patients (23.3%). Individuals with either a single or multiple polymorphism were approximately two times more likely to have CD than those with no polymorphism. Patients with multiple polymorphisms had significantly more stricturing and penetrating disease. Conclusion: NOD2/CARD15 gene polymorphisms were significantly associated with an increased risk of disease and aggressive phenotypes among the Kuwaiti CD population.http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2021;volume=27;issue=4;spage=249;epage=256;aulast=Abdelnabycrohn'skuwaitnod2/card15polymorphisms |
| spellingShingle | Hassan Abdelnaby Ndeye Coumba Ndiaye Ferdinando D'Amico Ahmed Mahmoud Fouad Sameh Hassan Alaa Elshafey Wafaa Al Hashash Mohammed Faisal Yousef Alshamali Talal Al-Taweel Laurent Peyrin-Biroulet NOD2/CARD15 polymorphisms (P268S, IVS8+158, G908R, L1007fs, R702W) among Kuwaiti patients with Crohn's disease: A case-control study The Saudi Journal of Gastroenterology crohn's kuwait nod2/card15 polymorphisms |
| title | NOD2/CARD15 polymorphisms (P268S, IVS8+158, G908R, L1007fs, R702W) among Kuwaiti patients with Crohn's disease: A case-control study |
| title_full | NOD2/CARD15 polymorphisms (P268S, IVS8+158, G908R, L1007fs, R702W) among Kuwaiti patients with Crohn's disease: A case-control study |
| title_fullStr | NOD2/CARD15 polymorphisms (P268S, IVS8+158, G908R, L1007fs, R702W) among Kuwaiti patients with Crohn's disease: A case-control study |
| title_full_unstemmed | NOD2/CARD15 polymorphisms (P268S, IVS8+158, G908R, L1007fs, R702W) among Kuwaiti patients with Crohn's disease: A case-control study |
| title_short | NOD2/CARD15 polymorphisms (P268S, IVS8+158, G908R, L1007fs, R702W) among Kuwaiti patients with Crohn's disease: A case-control study |
| title_sort | nod2 card15 polymorphisms p268s ivs8 158 g908r l1007fs r702w among kuwaiti patients with crohn s disease a case control study |
| topic | crohn's kuwait nod2/card15 polymorphisms |
| url | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2021;volume=27;issue=4;spage=249;epage=256;aulast=Abdelnaby |
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