Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk
The milk ring test is a detection assay for antibodies against <i>Brucella</i> in bovine milk. It has good sensitivity but tends to give false positive results. In this study, we standardized the application of the fluorescence polarization assay (FPA) for the detection of antibodies aga...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/6/303 |
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| author | Dianelys Sotolongo-Rodríguez Ricardo Gomez-Flores Magda Celina Navarro-Soto Beatriz Arellano-Reynoso Patricia Tamez-Guerra Carlos Ramírez-Pfeiffer |
| author_facet | Dianelys Sotolongo-Rodríguez Ricardo Gomez-Flores Magda Celina Navarro-Soto Beatriz Arellano-Reynoso Patricia Tamez-Guerra Carlos Ramírez-Pfeiffer |
| author_sort | Dianelys Sotolongo-Rodríguez |
| collection | DOAJ |
| description | The milk ring test is a detection assay for antibodies against <i>Brucella</i> in bovine milk. It has good sensitivity but tends to give false positive results. In this study, we standardized the application of the fluorescence polarization assay (FPA) for the detection of antibodies against <i>B.</i><i>melitensis</i> in goat milk. We obtained negative serum and milk samples from healthy goat flocks in the northern zone of Nuevo León. Positive milk and negative, weak, and strong controls were obtained by mixing volumes of positive control serum with negative control milk. Milk samples were treated with citric acid, after which an FPA was performed. Results were then compared with the Rose Bengal test and the FPA in serum. Milk treatment allowed the quantification of antibodies in samples. Significant differences were found between the 2%, 4%, and 6% groups, compared with the control group (F3, 67 = 17.45, <i>p</i> < 0.0001) but not between the 2% and 4% groups (<i>p</i> = 0.0718). The cut-off value was 74.1 mP, with a sensitivity (Se) of 95% and a specificity (Sp) of 100%. Se and Sp values in field milk samples were 84% and 74.55%, respectively. Despite the FPA test on milk samples showed lower Se and Sp than the FPA test on serum samples, its cutoff may be adjusted. It may be recommended as a screening test in goat milk and become useful for the control and eradication of the disease. |
| first_indexed | 2024-03-09T22:15:36Z |
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| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T22:15:36Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-b7b45d235ef34544a23c168c45afe8a92023-11-23T19:23:40ZengMDPI AGVeterinary Sciences2306-73812022-06-019630310.3390/vetsci9060303Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat MilkDianelys Sotolongo-Rodríguez0Ricardo Gomez-Flores1Magda Celina Navarro-Soto2Beatriz Arellano-Reynoso3Patricia Tamez-Guerra4Carlos Ramírez-Pfeiffer5Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza C.P. 66450, Nuevo León, MexicoDepartamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza C.P. 66450, Nuevo León, MexicoDepartamento de Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nuevo León, Escobedo C.P. 66054, Nuevo León, MexicoDepartamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán C.P. 04510, Ciudad de México, MexicoDepartamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza C.P. 66450, Nuevo León, MexicoCoordinación de Investigación Institucional, Universidad México Americana del Norte, Ciudad Reynosa C.P. 88640, Tamaulipas, MexicoThe milk ring test is a detection assay for antibodies against <i>Brucella</i> in bovine milk. It has good sensitivity but tends to give false positive results. In this study, we standardized the application of the fluorescence polarization assay (FPA) for the detection of antibodies against <i>B.</i><i>melitensis</i> in goat milk. We obtained negative serum and milk samples from healthy goat flocks in the northern zone of Nuevo León. Positive milk and negative, weak, and strong controls were obtained by mixing volumes of positive control serum with negative control milk. Milk samples were treated with citric acid, after which an FPA was performed. Results were then compared with the Rose Bengal test and the FPA in serum. Milk treatment allowed the quantification of antibodies in samples. Significant differences were found between the 2%, 4%, and 6% groups, compared with the control group (F3, 67 = 17.45, <i>p</i> < 0.0001) but not between the 2% and 4% groups (<i>p</i> = 0.0718). The cut-off value was 74.1 mP, with a sensitivity (Se) of 95% and a specificity (Sp) of 100%. Se and Sp values in field milk samples were 84% and 74.55%, respectively. Despite the FPA test on milk samples showed lower Se and Sp than the FPA test on serum samples, its cutoff may be adjusted. It may be recommended as a screening test in goat milk and become useful for the control and eradication of the disease.https://www.mdpi.com/2306-7381/9/6/303brucellosisfluorescence polarization assaygoat milkdiagnosisepidemiology |
| spellingShingle | Dianelys Sotolongo-Rodríguez Ricardo Gomez-Flores Magda Celina Navarro-Soto Beatriz Arellano-Reynoso Patricia Tamez-Guerra Carlos Ramírez-Pfeiffer Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk Veterinary Sciences brucellosis fluorescence polarization assay goat milk diagnosis epidemiology |
| title | Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk |
| title_full | Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk |
| title_fullStr | Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk |
| title_full_unstemmed | Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk |
| title_short | Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk |
| title_sort | evaluation of the fluorescence polarization assay for the diagnosis of brucellosis in goat milk |
| topic | brucellosis fluorescence polarization assay goat milk diagnosis epidemiology |
| url | https://www.mdpi.com/2306-7381/9/6/303 |
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