Bacteriological Evaluation of Aloe vera L. Fresh Gel on Experimental Infected Full-Thickness Open Wounds Induced with Staphylococcus aureus in Dogs
Objective - to evaluate both in-vivo and in-vitro antibacterial property of <em>Aloe vera</em>.<em>L</em> fresh gel.<br /> Design - experimental study.<br /> Animals - five adult male mixed breed dogs aged 2-4 years.<br /> Procedures - for in-vivo experiment...
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| Format: | Article |
| Language: | English |
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Iranian Veterinary Surgery Association (IVSA)
2012-08-01
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| Series: | Iranian Journal of Veterinary Surgery |
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| Online Access: | http://www.ivsajournals.com/article_3124_a81f5c2ce2efbe20ba9e0244aa5f6429.pdf |
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| author | Mehdi Rivandi Mohammad Reza Emami Mehrnaz Rad Hossein Kazemi Mehrjerdi Mohammad Azizzadeh Samaneh Ghasemi |
| author_facet | Mehdi Rivandi Mohammad Reza Emami Mehrnaz Rad Hossein Kazemi Mehrjerdi Mohammad Azizzadeh Samaneh Ghasemi |
| author_sort | Mehdi Rivandi |
| collection | DOAJ |
| description | Objective - to evaluate both in-vivo and in-vitro antibacterial property of <em>Aloe vera</em>.<em>L</em> fresh gel.<br /> Design - experimental study.<br /> Animals - five adult male mixed breed dogs aged 2-4 years.<br /> Procedures - for in-vivo experiment, under general anesthesia and sterile condition 8 symmetrical full-thickness wounds measured 2 cm×2 cm were surgically created on the back of each dogs on 0, 7, 13 and 21 days of study. After wound creation, 1 ml fluid contains 105 CFU <em>Staphylococcus aureus</em> was inoculated on each wound. Right wounds in all dogs were covered with 1 ml <em>Aloe vera</em> fresh gel whereas the left wounds were not received any treatment. Antibacterial activity of <em>Aloe vera</em> gel was evaluated on days 7, 15, 21 and 28 by wound biopsies. Antimicrobial susceptibility test was applied for in-vitro evaluation. For this purpose, measurement of inhibition zone of <em>Aloe vera </em>gel was compared to some commercially available antibiotic discs.<br /> Results - no significant differences were seen by counting viable bacteria between the treatment and control wounds at the particular days of the period study (P > 0.05). The gel inhibited the growth of <em>S.aureus </em>by appearance of 12 mm inhibitory zone.<br /> Conclusions and Clinical Relevance - <em>Aloe vera</em> has antibacterial property against <em>S.areus</em>. |
| first_indexed | 2024-12-10T04:35:41Z |
| format | Article |
| id | doaj.art-b7b6d6ecd9474db19e3b677232111540 |
| institution | Directory Open Access Journal |
| issn | 2008-3033 2008-3033 |
| language | English |
| last_indexed | 2024-12-10T04:35:41Z |
| publishDate | 2012-08-01 |
| publisher | Iranian Veterinary Surgery Association (IVSA) |
| record_format | Article |
| series | Iranian Journal of Veterinary Surgery |
| spelling | doaj.art-b7b6d6ecd9474db19e3b6772321115402022-12-22T02:02:01ZengIranian Veterinary Surgery Association (IVSA)Iranian Journal of Veterinary Surgery2008-30332008-30332012-08-01071-275843124Bacteriological Evaluation of Aloe vera L. Fresh Gel on Experimental Infected Full-Thickness Open Wounds Induced with Staphylococcus aureus in DogsMehdi Rivandi0Mohammad Reza Emami1Mehrnaz Rad2Hossein Kazemi Mehrjerdi3Mohammad Azizzadeh4Samaneh Ghasemi5Gratuated Student, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranObjective - to evaluate both in-vivo and in-vitro antibacterial property of <em>Aloe vera</em>.<em>L</em> fresh gel.<br /> Design - experimental study.<br /> Animals - five adult male mixed breed dogs aged 2-4 years.<br /> Procedures - for in-vivo experiment, under general anesthesia and sterile condition 8 symmetrical full-thickness wounds measured 2 cm×2 cm were surgically created on the back of each dogs on 0, 7, 13 and 21 days of study. After wound creation, 1 ml fluid contains 105 CFU <em>Staphylococcus aureus</em> was inoculated on each wound. Right wounds in all dogs were covered with 1 ml <em>Aloe vera</em> fresh gel whereas the left wounds were not received any treatment. Antibacterial activity of <em>Aloe vera</em> gel was evaluated on days 7, 15, 21 and 28 by wound biopsies. Antimicrobial susceptibility test was applied for in-vitro evaluation. For this purpose, measurement of inhibition zone of <em>Aloe vera </em>gel was compared to some commercially available antibiotic discs.<br /> Results - no significant differences were seen by counting viable bacteria between the treatment and control wounds at the particular days of the period study (P > 0.05). The gel inhibited the growth of <em>S.aureus </em>by appearance of 12 mm inhibitory zone.<br /> Conclusions and Clinical Relevance - <em>Aloe vera</em> has antibacterial property against <em>S.areus</em>.http://www.ivsajournals.com/article_3124_a81f5c2ce2efbe20ba9e0244aa5f6429.pdfAloe veraInfected full-thickness woundsStaphylococcus aureus |
| spellingShingle | Mehdi Rivandi Mohammad Reza Emami Mehrnaz Rad Hossein Kazemi Mehrjerdi Mohammad Azizzadeh Samaneh Ghasemi Bacteriological Evaluation of Aloe vera L. Fresh Gel on Experimental Infected Full-Thickness Open Wounds Induced with Staphylococcus aureus in Dogs Iranian Journal of Veterinary Surgery Aloe vera Infected full-thickness wounds Staphylococcus aureus |
| title | Bacteriological Evaluation of Aloe vera L. Fresh Gel on Experimental Infected Full-Thickness Open Wounds Induced with Staphylococcus aureus in Dogs |
| title_full | Bacteriological Evaluation of Aloe vera L. Fresh Gel on Experimental Infected Full-Thickness Open Wounds Induced with Staphylococcus aureus in Dogs |
| title_fullStr | Bacteriological Evaluation of Aloe vera L. Fresh Gel on Experimental Infected Full-Thickness Open Wounds Induced with Staphylococcus aureus in Dogs |
| title_full_unstemmed | Bacteriological Evaluation of Aloe vera L. Fresh Gel on Experimental Infected Full-Thickness Open Wounds Induced with Staphylococcus aureus in Dogs |
| title_short | Bacteriological Evaluation of Aloe vera L. Fresh Gel on Experimental Infected Full-Thickness Open Wounds Induced with Staphylococcus aureus in Dogs |
| title_sort | bacteriological evaluation of aloe vera l fresh gel on experimental infected full thickness open wounds induced with staphylococcus aureus in dogs |
| topic | Aloe vera Infected full-thickness wounds Staphylococcus aureus |
| url | http://www.ivsajournals.com/article_3124_a81f5c2ce2efbe20ba9e0244aa5f6429.pdf |
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