Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering
Soft tissue engineering has been seeking ways to mimic the natural extracellular microenvironment that allows cells to migrate and proliferate to regenerate new tissue. Therefore, the reconstruction of soft tissue requires a scaffold possessing the extracellular matrix (ECM)-mimicking fibrous struct...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/9/1977 |
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| author | Kexin Nie Shanshan Han Jianmin Yang Qingqing Sun Xiaofeng Wang Xiaomeng Li Qian Li |
| author_facet | Kexin Nie Shanshan Han Jianmin Yang Qingqing Sun Xiaofeng Wang Xiaomeng Li Qian Li |
| author_sort | Kexin Nie |
| collection | DOAJ |
| description | Soft tissue engineering has been seeking ways to mimic the natural extracellular microenvironment that allows cells to migrate and proliferate to regenerate new tissue. Therefore, the reconstruction of soft tissue requires a scaffold possessing the extracellular matrix (ECM)-mimicking fibrous structure and elastic property, which affect the cell functions and tissue regeneration. Herein, an effective method for fabricating nanofibrous hydrogel for soft tissue engineering is demonstrated using gelatin–hydroxyphenylpropionic acid (Gel–HPA) by electrospinning and enzymatic crosslinking. Gel–HPA fibrous hydrogel was prepared by crosslinking the electrospun fibers in ethanol-water solution with an optimized concentration of horseradish peroxidase (HRP) and H<sub>2</sub>O<sub>2</sub>. The prepared fibrous hydrogel held the soft and elastic mechanical property of hydrogels and the three-dimensional (3D) fibrous structure of electrospun fibers. It was proven that the hydrogel scaffolds were biocompatible, improving the cellular adhesion, spreading, and proliferation. Moreover, the fibrous hydrogel showed rapid biodegradability and promoted angiogenesis in vivo. Overall, this study represents a novel biomimetic approach to generate Gel–HPA fibrous hydrogel scaffolds which have excellent potential in soft tissue regeneration applications. |
| first_indexed | 2024-03-10T16:42:16Z |
| format | Article |
| id | doaj.art-b7b7ee6aeb9542a3a7abb27e23466ac2 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T16:42:16Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-b7b7ee6aeb9542a3a7abb27e23466ac22023-11-20T11:59:20ZengMDPI AGPolymers2073-43602020-08-01129197710.3390/polym12091977Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue EngineeringKexin Nie0Shanshan Han1Jianmin Yang2Qingqing Sun3Xiaofeng Wang4Xiaomeng Li5Qian Li6School of Mechanics and Safety Engineering, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Mechanics and Safety Engineering, Zhengzhou University, Zhengzhou 450001, ChinaCollege of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, ChinaCenter for Functional Sensor and Actuator, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, JapanSchool of Mechanics and Safety Engineering, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Mechanics and Safety Engineering, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Mechanics and Safety Engineering, Zhengzhou University, Zhengzhou 450001, ChinaSoft tissue engineering has been seeking ways to mimic the natural extracellular microenvironment that allows cells to migrate and proliferate to regenerate new tissue. Therefore, the reconstruction of soft tissue requires a scaffold possessing the extracellular matrix (ECM)-mimicking fibrous structure and elastic property, which affect the cell functions and tissue regeneration. Herein, an effective method for fabricating nanofibrous hydrogel for soft tissue engineering is demonstrated using gelatin–hydroxyphenylpropionic acid (Gel–HPA) by electrospinning and enzymatic crosslinking. Gel–HPA fibrous hydrogel was prepared by crosslinking the electrospun fibers in ethanol-water solution with an optimized concentration of horseradish peroxidase (HRP) and H<sub>2</sub>O<sub>2</sub>. The prepared fibrous hydrogel held the soft and elastic mechanical property of hydrogels and the three-dimensional (3D) fibrous structure of electrospun fibers. It was proven that the hydrogel scaffolds were biocompatible, improving the cellular adhesion, spreading, and proliferation. Moreover, the fibrous hydrogel showed rapid biodegradability and promoted angiogenesis in vivo. Overall, this study represents a novel biomimetic approach to generate Gel–HPA fibrous hydrogel scaffolds which have excellent potential in soft tissue regeneration applications.https://www.mdpi.com/2073-4360/12/9/1977fibrous hydrogelenzymatic crosslinkingsoft tissue engineering |
| spellingShingle | Kexin Nie Shanshan Han Jianmin Yang Qingqing Sun Xiaofeng Wang Xiaomeng Li Qian Li Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering Polymers fibrous hydrogel enzymatic crosslinking soft tissue engineering |
| title | Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering |
| title_full | Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering |
| title_fullStr | Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering |
| title_full_unstemmed | Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering |
| title_short | Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering |
| title_sort | enzyme crosslinked electrospun fibrous gelatin hydrogel for potential soft tissue engineering |
| topic | fibrous hydrogel enzymatic crosslinking soft tissue engineering |
| url | https://www.mdpi.com/2073-4360/12/9/1977 |
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