Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)
DNA damage detection in Ephestia kuehniella by RAPD-PCR Abstract - The random amplified polymorphic DNA (RAPD) assay is a useful method for detecting genotoxin-induced DNA damage. In the present study, this assay was evaluated to measure the essential oil of Prangos ferulacea-induced DNA ch...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2015-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400029E.pdf |
| _version_ | 1818274200229511168 |
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| author | Ercan Fahriye Sumer |
| author_facet | Ercan Fahriye Sumer |
| author_sort | Ercan Fahriye Sumer |
| collection | DOAJ |
| description | DNA damage detection in Ephestia kuehniella by RAPD-PCR Abstract - The random
amplified polymorphic DNA (RAPD) assay is a useful method for detecting
genotoxin-induced DNA damage. In the present study, this assay was evaluated
to measure the essential oil of Prangos ferulacea-induced DNA changes in
Ephestia kuehniella Zeller larvae. The third instar larvae of E. kuehniella
were exposed to 500 μl L-1 essential oil for 24 h. Forty random primers were
used for RAPD-PCR of which eleven produced unique polymorphic band patterns.
In comparison with control larvae, the essential oil-treated larvae showed
greater changes in RAPD profiles. This is the first report of an analysis of
the genotoxic effect of P. ferulacea essential oil against E. kuehniella
larvae using RAPD-PCR. |
| first_indexed | 2024-12-12T22:10:04Z |
| format | Article |
| id | doaj.art-b7b85569fa6d469e98d141ac9590124b |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-12T22:10:04Z |
| publishDate | 2015-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-b7b85569fa6d469e98d141ac9590124b2022-12-22T00:10:17ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392015-01-0167123523910.2298/ABS140903029E0354-46641400029EUse of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)Ercan Fahriye Sumer0Bozok University, Faculty of Science and Art, Department of Biology, Yozgat, TurkeyDNA damage detection in Ephestia kuehniella by RAPD-PCR Abstract - The random amplified polymorphic DNA (RAPD) assay is a useful method for detecting genotoxin-induced DNA damage. In the present study, this assay was evaluated to measure the essential oil of Prangos ferulacea-induced DNA changes in Ephestia kuehniella Zeller larvae. The third instar larvae of E. kuehniella were exposed to 500 μl L-1 essential oil for 24 h. Forty random primers were used for RAPD-PCR of which eleven produced unique polymorphic band patterns. In comparison with control larvae, the essential oil-treated larvae showed greater changes in RAPD profiles. This is the first report of an analysis of the genotoxic effect of P. ferulacea essential oil against E. kuehniella larvae using RAPD-PCR.http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400029E.pdfRAPD-PCREphestia kuehniellaPrangos ferulaceaessential oilDNA damage |
| spellingShingle | Ercan Fahriye Sumer Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) Archives of Biological Sciences RAPD-PCR Ephestia kuehniella Prangos ferulacea essential oil DNA damage |
| title | Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) |
| title_full | Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) |
| title_fullStr | Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) |
| title_full_unstemmed | Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) |
| title_short | Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) |
| title_sort | use of random amplified polymorphic dna rapd to detect dna damage induced by prangos ferulacea umbelliferae essential oil against the mediterranean flour moth ephestia kuehniella zeller lepidoptera pyralidae |
| topic | RAPD-PCR Ephestia kuehniella Prangos ferulacea essential oil DNA damage |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400029E.pdf |
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