Nuclear p120-catenin regulates the anoikis resistance of mouse lobular breast cancer cells through Kaiso-dependent Wnt11 expression
E-cadherin inactivation underpins the progression of invasive lobular breast carcinoma (ILC). In ILC, p120-catenin (p120) translocates to the cytosol where it controls anchorage independence through the Rho-Rock signaling pathway, a key mechanism driving tumor growth and metastasis. We now demonstra...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2015-04-01
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| Series: | Disease Models & Mechanisms |
| Subjects: | |
| Online Access: | http://dmm.biologists.org/content/8/4/373 |
| _version_ | 1828925201723686912 |
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| author | Robert A. H. van de Ven Milou Tenhagen Wouter Meuleman Jeske J. G. van Riel Ron C. J. Schackmann Patrick W. B. Derksen |
| author_facet | Robert A. H. van de Ven Milou Tenhagen Wouter Meuleman Jeske J. G. van Riel Ron C. J. Schackmann Patrick W. B. Derksen |
| author_sort | Robert A. H. van de Ven |
| collection | DOAJ |
| description | E-cadherin inactivation underpins the progression of invasive lobular breast carcinoma (ILC). In ILC, p120-catenin (p120) translocates to the cytosol where it controls anchorage independence through the Rho-Rock signaling pathway, a key mechanism driving tumor growth and metastasis. We now demonstrate that anchorage-independent ILC cells show an increase in nuclear p120, which results in relief of transcriptional repression by Kaiso. To identify the Kaiso target genes that control anchorage independence we performed genome-wide mRNA profiling on anoikis-resistant mouse ILC cells, and identified 29 candidate target genes, including the established Kaiso target Wnt11. Our data indicate that anchorage-independent upregulation of Wnt11 in ILC cells is controlled by nuclear p120 through inhibition of Kaiso-mediated transcriptional repression. Finally, we show that Wnt11 promotes activation of RhoA, which causes ILC anoikis resistance. Our findings thereby establish a mechanistic link between E-cadherin loss and subsequent control of Rho-driven anoikis resistance through p120- and Kaiso-dependent expression of Wnt11. |
| first_indexed | 2024-12-13T23:10:48Z |
| format | Article |
| id | doaj.art-b7bbaceb3afd41468abf0fd1753b7c88 |
| institution | Directory Open Access Journal |
| issn | 1754-8403 1754-8411 |
| language | English |
| last_indexed | 2024-12-13T23:10:48Z |
| publishDate | 2015-04-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Disease Models & Mechanisms |
| spelling | doaj.art-b7bbaceb3afd41468abf0fd1753b7c882022-12-21T23:28:07ZengThe Company of BiologistsDisease Models & Mechanisms1754-84031754-84112015-04-018437338410.1242/dmm.018648018648Nuclear p120-catenin regulates the anoikis resistance of mouse lobular breast cancer cells through Kaiso-dependent Wnt11 expressionRobert A. H. van de VenMilou TenhagenWouter MeulemanJeske J. G. van RielRon C. J. SchackmannPatrick W. B. DerksenE-cadherin inactivation underpins the progression of invasive lobular breast carcinoma (ILC). In ILC, p120-catenin (p120) translocates to the cytosol where it controls anchorage independence through the Rho-Rock signaling pathway, a key mechanism driving tumor growth and metastasis. We now demonstrate that anchorage-independent ILC cells show an increase in nuclear p120, which results in relief of transcriptional repression by Kaiso. To identify the Kaiso target genes that control anchorage independence we performed genome-wide mRNA profiling on anoikis-resistant mouse ILC cells, and identified 29 candidate target genes, including the established Kaiso target Wnt11. Our data indicate that anchorage-independent upregulation of Wnt11 in ILC cells is controlled by nuclear p120 through inhibition of Kaiso-mediated transcriptional repression. Finally, we show that Wnt11 promotes activation of RhoA, which causes ILC anoikis resistance. Our findings thereby establish a mechanistic link between E-cadherin loss and subsequent control of Rho-driven anoikis resistance through p120- and Kaiso-dependent expression of Wnt11.http://dmm.biologists.org/content/8/4/373p120-cateninKaisoBreast cancer metastasisAnoikis resistance |
| spellingShingle | Robert A. H. van de Ven Milou Tenhagen Wouter Meuleman Jeske J. G. van Riel Ron C. J. Schackmann Patrick W. B. Derksen Nuclear p120-catenin regulates the anoikis resistance of mouse lobular breast cancer cells through Kaiso-dependent Wnt11 expression Disease Models & Mechanisms p120-catenin Kaiso Breast cancer metastasis Anoikis resistance |
| title | Nuclear p120-catenin regulates the anoikis resistance of mouse lobular breast cancer cells through Kaiso-dependent Wnt11 expression |
| title_full | Nuclear p120-catenin regulates the anoikis resistance of mouse lobular breast cancer cells through Kaiso-dependent Wnt11 expression |
| title_fullStr | Nuclear p120-catenin regulates the anoikis resistance of mouse lobular breast cancer cells through Kaiso-dependent Wnt11 expression |
| title_full_unstemmed | Nuclear p120-catenin regulates the anoikis resistance of mouse lobular breast cancer cells through Kaiso-dependent Wnt11 expression |
| title_short | Nuclear p120-catenin regulates the anoikis resistance of mouse lobular breast cancer cells through Kaiso-dependent Wnt11 expression |
| title_sort | nuclear p120 catenin regulates the anoikis resistance of mouse lobular breast cancer cells through kaiso dependent wnt11 expression |
| topic | p120-catenin Kaiso Breast cancer metastasis Anoikis resistance |
| url | http://dmm.biologists.org/content/8/4/373 |
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