Purification and Enzymatic Properties of Soluble and Membrane-Bound Polyphenol Oxidase from Agaricus bisporus

Purification and Enzymatic Properties of Soluble and Membrane-Bound Polyphenol Oxidase from Agaricus bisporus

An extraction and purification method for soluble polyphenol oxidase (sPPO) and membrane-bound polyphenol oxidases (mPPO) from Agaricus bisporus was developed involving temperature-induced phase partitioning, fractional precipitation with ammonium sulfate, and DEAE anion exchange chromatography. The...

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Bibliographic Details
Main Author:	HE Xingxing, LEI Jing, GUAN Wenqiang
Format:	Article
Language:	English
Published:	China Food Publishing Company 2023-05-01
Series:	Shipin Kexue
Subjects:	agaricus bisporus; soluble polyphenol oxidase; membrane-bound polyphenol oxidase; purification
Online Access:	https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-10-022.pdf

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