On the use of thermal forces to probe kinesin’s response to force
The stepping dynamics of cytoskeletal motor proteins determines the dynamics of cargo transport. In its native cellular environment, a molecular motor is subject to forces from several sources including thermal forces and forces ensuing from the interaction with other motors bound to the same cargo....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1260914/full |
| _version_ | 1797644363520540672 |
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| author | Chuan Chang Tiantian Zheng Guilherme Nettesheim Hayoung Song Changhyun Cho Samuele Crespi George Shubeita |
| author_facet | Chuan Chang Tiantian Zheng Guilherme Nettesheim Hayoung Song Changhyun Cho Samuele Crespi George Shubeita |
| author_sort | Chuan Chang |
| collection | DOAJ |
| description | The stepping dynamics of cytoskeletal motor proteins determines the dynamics of cargo transport. In its native cellular environment, a molecular motor is subject to forces from several sources including thermal forces and forces ensuing from the interaction with other motors bound to the same cargo. Understanding how the individual motors respond to these forces can allow us to predict how they move their cargo when part of a team. Here, using simulation, we show that details of how the kinesin motor responds to small assisting forces–which, at the moment, are not experimentally constrained-can lead to significant changes in cargo dynamics. Using different models of the force-dependent detachment probability of the kinesin motor leads to different predictions on the run-length of the cargo they carry. These differences emerge from the thermal forces acting on the cargo and transmitted to the motor through the motor tail that tethers the motor head to the microtubule. We show that these differences appear for cargo carried by individual motors or motor teams, and use our findings to propose the use of thermal forces as a probe of kinesin’s response to force in this otherwise inaccessible force regime. |
| first_indexed | 2024-03-11T14:29:28Z |
| format | Article |
| id | doaj.art-b7bf768e0c6c4d16a939a15dc620e6f6 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-03-11T14:29:28Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-b7bf768e0c6c4d16a939a15dc620e6f62023-10-31T11:06:29ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2023-10-011010.3389/fmolb.2023.12609141260914On the use of thermal forces to probe kinesin’s response to forceChuan Chang0Tiantian Zheng1Guilherme Nettesheim2Hayoung Song3Changhyun Cho4Samuele Crespi5George Shubeita6Physics Program, New York University Abu Dhabi, Abu Dhabi, United Arab EmiratesPhysics Program, New York University Abu Dhabi, Abu Dhabi, United Arab EmiratesCavendish Laboratory, Department of Physics, University of Cambridge, Cambridge, United KingdomPhysics Program, New York University Abu Dhabi, Abu Dhabi, United Arab EmiratesPhysics Program, New York University Abu Dhabi, Abu Dhabi, United Arab EmiratesPhysics Program, New York University Abu Dhabi, Abu Dhabi, United Arab EmiratesPhysics Program, New York University Abu Dhabi, Abu Dhabi, United Arab EmiratesThe stepping dynamics of cytoskeletal motor proteins determines the dynamics of cargo transport. In its native cellular environment, a molecular motor is subject to forces from several sources including thermal forces and forces ensuing from the interaction with other motors bound to the same cargo. Understanding how the individual motors respond to these forces can allow us to predict how they move their cargo when part of a team. Here, using simulation, we show that details of how the kinesin motor responds to small assisting forces–which, at the moment, are not experimentally constrained-can lead to significant changes in cargo dynamics. Using different models of the force-dependent detachment probability of the kinesin motor leads to different predictions on the run-length of the cargo they carry. These differences emerge from the thermal forces acting on the cargo and transmitted to the motor through the motor tail that tethers the motor head to the microtubule. We show that these differences appear for cargo carried by individual motors or motor teams, and use our findings to propose the use of thermal forces as a probe of kinesin’s response to force in this otherwise inaccessible force regime.https://www.frontiersin.org/articles/10.3389/fmolb.2023.1260914/fullmolecular motorskinesinintracellular transportmotor force responsemotor runlengththermal forces |
| spellingShingle | Chuan Chang Tiantian Zheng Guilherme Nettesheim Hayoung Song Changhyun Cho Samuele Crespi George Shubeita On the use of thermal forces to probe kinesin’s response to force Frontiers in Molecular Biosciences molecular motors kinesin intracellular transport motor force response motor runlength thermal forces |
| title | On the use of thermal forces to probe kinesin’s response to force |
| title_full | On the use of thermal forces to probe kinesin’s response to force |
| title_fullStr | On the use of thermal forces to probe kinesin’s response to force |
| title_full_unstemmed | On the use of thermal forces to probe kinesin’s response to force |
| title_short | On the use of thermal forces to probe kinesin’s response to force |
| title_sort | on the use of thermal forces to probe kinesin s response to force |
| topic | molecular motors kinesin intracellular transport motor force response motor runlength thermal forces |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1260914/full |
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