Alternative Functional rad21 Paralogs in Fusarium oxysporum
Cohesin, the sister chromatid cohesion complex, is an essential complex that ensures faithful sister chromatid segregation in eukaryotes. It also participates in DNA repair, transcription and maintenance of chromosome structure. Mitotic cohesin is composed of Smc1, Smc3, Scc3, and Rad21/Mcd1. The me...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01370/full |
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| author | Manish Pareek Yael Almog Vinay Kumar Bari Einat Hazkani-Covo Itay Onn Shay Covo |
| author_facet | Manish Pareek Yael Almog Vinay Kumar Bari Einat Hazkani-Covo Itay Onn Shay Covo |
| author_sort | Manish Pareek |
| collection | DOAJ |
| description | Cohesin, the sister chromatid cohesion complex, is an essential complex that ensures faithful sister chromatid segregation in eukaryotes. It also participates in DNA repair, transcription and maintenance of chromosome structure. Mitotic cohesin is composed of Smc1, Smc3, Scc3, and Rad21/Mcd1. The meiotic cohesin complex contains Rec8, a Rad21 paralog and not Rad21 itself. Very little is known about sister chromatid cohesion in fungal plant pathogens. Fusarium oxysporum is an important fungal plant pathogen without known sexual life cycle. Here, we describe that F. oxysporum encodes for three Rad21 paralogs; Rad21, Rec8, and the first alternative Rad21 paralog in the phylum of ascomycete. This last paralog is found only in several fungal plant pathogens from the Fusarium family and thus termed rad21nc (non-conserved). Conserved rad21 (rad21c), rad21nc, and rec8 genes are expressed in F. oxysporum although the expression of rad21c is much higher than the other paralogs. F. oxysporum strains deleted for the rad21nc or rec8 genes were analyzed for their role in fungal life cycle. Δrad21nc and Δrec8 single mutants were proficient in sporulation, conidia germination, hyphal growth and pathogenicity under optimal growth conditions. Interestingly, Δrad21nc and Δrec8 single mutants germinate less effectively than wild type (WT) strains under DNA replication and mitosis stresses. We provide here the first genetic analysis of alternative rad21nc and rec8 paralogs in filamentous fungi. Our results suggest that rad21nc and rec8 may have a unique role in cell cycle related functions of F. oxysporum. |
| first_indexed | 2024-04-12T01:47:32Z |
| format | Article |
| id | doaj.art-b7c0e71ddcf242c5b30b9a53ffe3882c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T01:47:32Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b7c0e71ddcf242c5b30b9a53ffe3882c2022-12-22T03:53:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-06-011010.3389/fmicb.2019.01370432509Alternative Functional rad21 Paralogs in Fusarium oxysporumManish Pareek0Yael Almog1Vinay Kumar Bari2Einat Hazkani-Covo3Itay Onn4Shay Covo5Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Natural Sciences, Open University of Israel, Ra’anana, IsraelThe Azrieli Faculty of Medicine, Bar Ilan University, Safed, IsraelDepartment of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelCohesin, the sister chromatid cohesion complex, is an essential complex that ensures faithful sister chromatid segregation in eukaryotes. It also participates in DNA repair, transcription and maintenance of chromosome structure. Mitotic cohesin is composed of Smc1, Smc3, Scc3, and Rad21/Mcd1. The meiotic cohesin complex contains Rec8, a Rad21 paralog and not Rad21 itself. Very little is known about sister chromatid cohesion in fungal plant pathogens. Fusarium oxysporum is an important fungal plant pathogen without known sexual life cycle. Here, we describe that F. oxysporum encodes for three Rad21 paralogs; Rad21, Rec8, and the first alternative Rad21 paralog in the phylum of ascomycete. This last paralog is found only in several fungal plant pathogens from the Fusarium family and thus termed rad21nc (non-conserved). Conserved rad21 (rad21c), rad21nc, and rec8 genes are expressed in F. oxysporum although the expression of rad21c is much higher than the other paralogs. F. oxysporum strains deleted for the rad21nc or rec8 genes were analyzed for their role in fungal life cycle. Δrad21nc and Δrec8 single mutants were proficient in sporulation, conidia germination, hyphal growth and pathogenicity under optimal growth conditions. Interestingly, Δrad21nc and Δrec8 single mutants germinate less effectively than wild type (WT) strains under DNA replication and mitosis stresses. We provide here the first genetic analysis of alternative rad21nc and rec8 paralogs in filamentous fungi. Our results suggest that rad21nc and rec8 may have a unique role in cell cycle related functions of F. oxysporum.https://www.frontiersin.org/article/10.3389/fmicb.2019.01370/fullcohesinMcd1Rad21Rec8Fusarium oxysporumcell cycle |
| spellingShingle | Manish Pareek Yael Almog Vinay Kumar Bari Einat Hazkani-Covo Itay Onn Shay Covo Alternative Functional rad21 Paralogs in Fusarium oxysporum Frontiers in Microbiology cohesin Mcd1 Rad21 Rec8 Fusarium oxysporum cell cycle |
| title | Alternative Functional rad21 Paralogs in Fusarium oxysporum |
| title_full | Alternative Functional rad21 Paralogs in Fusarium oxysporum |
| title_fullStr | Alternative Functional rad21 Paralogs in Fusarium oxysporum |
| title_full_unstemmed | Alternative Functional rad21 Paralogs in Fusarium oxysporum |
| title_short | Alternative Functional rad21 Paralogs in Fusarium oxysporum |
| title_sort | alternative functional rad21 paralogs in fusarium oxysporum |
| topic | cohesin Mcd1 Rad21 Rec8 Fusarium oxysporum cell cycle |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01370/full |
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