Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)
Chromatin is a dynamic structure comprising of DNA and proteins. Its unique nature not only help to pack the DNA tightly within the cell but also is pivotal in regulating gene expression DNA replication. Furthermore it also protects the DNA from being damaged. Various proteins are involved in making...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/21/6694 |
| _version_ | 1797512022796009472 |
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| author | Niamat Khan Sidra Shahid Abdul R. Asif |
| author_facet | Niamat Khan Sidra Shahid Abdul R. Asif |
| author_sort | Niamat Khan |
| collection | DOAJ |
| description | Chromatin is a dynamic structure comprising of DNA and proteins. Its unique nature not only help to pack the DNA tightly within the cell but also is pivotal in regulating gene expression DNA replication. Furthermore it also protects the DNA from being damaged. Various proteins are involved in making a specific complex within a chromatin and the knowledge about these interacting partners is helpful to enhance our understanding about the pathophysiology of various chromatin associated diseases. Moreover, it could also help us to identify new drug targets and design more effective remedies. Due to the existence of chromatin in different forms under various physiological conditions it is hard to develop a single strategy to study chromatin associated proteins under all conditions. In our current review, we tried to provide an overview and comparative analysis of the strategies currently adopted to capture the DNA bounded protein complexes and their mass spectrometric identification and quantification. Precise information about the protein partners and their function in the DNA-protein complexes is crucial to design new and more effective therapeutic molecules against chromatin associated diseases. |
| first_indexed | 2024-03-10T05:55:07Z |
| format | Article |
| id | doaj.art-b7c33cef56524a038a479a14d709b6fd |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:55:07Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b7c33cef56524a038a479a14d709b6fd2023-11-22T21:25:09ZengMDPI AGMolecules1420-30492021-11-012621669410.3390/molecules26216694Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)Niamat Khan0Sidra Shahid1Abdul R. Asif2Institute for Clinical Chemistry/UMG-Laboratories, University Medical Centre, Robert-Koch-Str. 40, 37075 Goettingen, GermanyInstitute for Clinical Chemistry/UMG-Laboratories, University Medical Centre, Robert-Koch-Str. 40, 37075 Goettingen, GermanyInstitute for Clinical Chemistry/UMG-Laboratories, University Medical Centre, Robert-Koch-Str. 40, 37075 Goettingen, GermanyChromatin is a dynamic structure comprising of DNA and proteins. Its unique nature not only help to pack the DNA tightly within the cell but also is pivotal in regulating gene expression DNA replication. Furthermore it also protects the DNA from being damaged. Various proteins are involved in making a specific complex within a chromatin and the knowledge about these interacting partners is helpful to enhance our understanding about the pathophysiology of various chromatin associated diseases. Moreover, it could also help us to identify new drug targets and design more effective remedies. Due to the existence of chromatin in different forms under various physiological conditions it is hard to develop a single strategy to study chromatin associated proteins under all conditions. In our current review, we tried to provide an overview and comparative analysis of the strategies currently adopted to capture the DNA bounded protein complexes and their mass spectrometric identification and quantification. Precise information about the protein partners and their function in the DNA-protein complexes is crucial to design new and more effective therapeutic molecules against chromatin associated diseases.https://www.mdpi.com/1420-3049/26/21/6694chromatineuchromatinheterochromatininterphasecentromeretelomere |
| spellingShingle | Niamat Khan Sidra Shahid Abdul R. Asif Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome) Molecules chromatin euchromatin heterochromatin interphase centromere telomere |
| title | Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome) |
| title_full | Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome) |
| title_fullStr | Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome) |
| title_full_unstemmed | Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome) |
| title_short | Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome) |
| title_sort | current analytical strategies in studying chromatin associated proteome chromatome |
| topic | chromatin euchromatin heterochromatin interphase centromere telomere |
| url | https://www.mdpi.com/1420-3049/26/21/6694 |
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