Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin
ABSTRACT Purpose: The active melanocytes in the skin were affected by hormones and ultraviolet (UV) irradiation. Licorice zinc has a whitening effect, which may have a prominent potential in the treatment of pigmented skin disease. Methods: Modeling chloasma C57BL/6J mice by daily progesterone inj...
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| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022-12-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022001000201&tlng=en |
| _version_ | 1797980109085343744 |
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| author | Jing-yan Wang Xing-yu Xie Ying Deng Hong-qiu Yang Xiao-shuang Du Ping Liu Yu Du |
| author_facet | Jing-yan Wang Xing-yu Xie Ying Deng Hong-qiu Yang Xiao-shuang Du Ping Liu Yu Du |
| author_sort | Jing-yan Wang |
| collection | DOAJ |
| description | ABSTRACT Purpose: The active melanocytes in the skin were affected by hormones and ultraviolet (UV) irradiation. Licorice zinc has a whitening effect, which may have a prominent potential in the treatment of pigmented skin disease. Methods: Modeling chloasma C57BL/6J mice by daily progesterone injection (15 mg/kg) and ultraviolet B (UVB) irradiation (λ = 312 nm, 2 h/day) for 30 days. Then, mice were given 0.65, 1.3, and 2.6 (g/kg) of licorice zinc and tranexamic acid 250 mg daily by oral administration for 14 days, respectively. Hematoxylin and eosin and Fontana-Masson staining, and Western blotting (WB) were performed to test the inhibitory of melanogenesis and activation of c-Jun-N-terminal (JNK)/p38 mitogen-activated protein kinases (MAPK) for licorice zinc. Melanogenesis was induced by α-melanocyte-stimulating hormone in vitro. Cell counting kit-8, melanin content determination, and WB were performed to verify the inhibitory effect of licorice zinc on melanogenesis. Results: The present study showed that licorice zinc decreased melanin formation, cutaneous tissue injury, and the phosphorylation of JNK and P38MAPK, which was caused by UVB irradiation in vivo. In vitro, licorice zinc showed opposite effects from JNK/p38 activator. Meanwhile, tyrosinase-related protein-1, tyrosinase, and microphthalmia-associated transcription factor were decreased too. Conclusions: Licorice zinc induced a decrease in melanin synthesis by inhibiting the JNK and the P38MAPK signaling pathway, suggesting licorice zinc is a potential agent of anti-chloasma. |
| first_indexed | 2024-04-11T05:50:12Z |
| format | Article |
| id | doaj.art-b7c7135a01794f5897933dca63747425 |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-04-11T05:50:12Z |
| publishDate | 2022-12-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-b7c7135a01794f5897933dca637474252022-12-22T04:42:06ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742022-12-01371010.1590/acb371002Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skinJing-yan Wanghttps://orcid.org/0000-0002-4954-1823Xing-yu Xiehttps://orcid.org/0000-0002-1630-8355Ying Denghttps://orcid.org/0000-0003-0696-5244Hong-qiu Yanghttps://orcid.org/0000-0003-2162-2290Xiao-shuang Duhttps://orcid.org/0000-0003-3161-1421Ping Liuhttps://orcid.org/0000-0003-3953-909XYu Duhttps://orcid.org/0000-0002-7522-5744ABSTRACT Purpose: The active melanocytes in the skin were affected by hormones and ultraviolet (UV) irradiation. Licorice zinc has a whitening effect, which may have a prominent potential in the treatment of pigmented skin disease. Methods: Modeling chloasma C57BL/6J mice by daily progesterone injection (15 mg/kg) and ultraviolet B (UVB) irradiation (λ = 312 nm, 2 h/day) for 30 days. Then, mice were given 0.65, 1.3, and 2.6 (g/kg) of licorice zinc and tranexamic acid 250 mg daily by oral administration for 14 days, respectively. Hematoxylin and eosin and Fontana-Masson staining, and Western blotting (WB) were performed to test the inhibitory of melanogenesis and activation of c-Jun-N-terminal (JNK)/p38 mitogen-activated protein kinases (MAPK) for licorice zinc. Melanogenesis was induced by α-melanocyte-stimulating hormone in vitro. Cell counting kit-8, melanin content determination, and WB were performed to verify the inhibitory effect of licorice zinc on melanogenesis. Results: The present study showed that licorice zinc decreased melanin formation, cutaneous tissue injury, and the phosphorylation of JNK and P38MAPK, which was caused by UVB irradiation in vivo. In vitro, licorice zinc showed opposite effects from JNK/p38 activator. Meanwhile, tyrosinase-related protein-1, tyrosinase, and microphthalmia-associated transcription factor were decreased too. Conclusions: Licorice zinc induced a decrease in melanin synthesis by inhibiting the JNK and the P38MAPK signaling pathway, suggesting licorice zinc is a potential agent of anti-chloasma.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022001000201&tlng=enMelanosisGlycyrrhizaZincMAP Kinase Signaling System |
| spellingShingle | Jing-yan Wang Xing-yu Xie Ying Deng Hong-qiu Yang Xiao-shuang Du Ping Liu Yu Du Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin Acta Cirúrgica Brasileira Melanosis Glycyrrhiza Zinc MAP Kinase Signaling System |
| title | Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin |
| title_full | Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin |
| title_fullStr | Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin |
| title_full_unstemmed | Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin |
| title_short | Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin |
| title_sort | licorice zinc suppresses melanogenesis via inhibiting the activation of p38mapk and jnk signaling pathway in c57bl 6j mice skin |
| topic | Melanosis Glycyrrhiza Zinc MAP Kinase Signaling System |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022001000201&tlng=en |
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