Characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver disease
In obese patients, non-alcoholic fatty liver (NAFLD) is common. However, whether there is a connection between the gut microbiota and the onset of NAFLD in obese people is yet unknown. Using quantitative real-time PCR, the microbiota of feces of the eligible 181 obese individuals was identified to c...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1113643/full |
| _version_ | 1828056479742033920 |
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| author | Li-ting Jin Ming-Zhi Xu Ming-Zhi Xu |
| author_facet | Li-ting Jin Ming-Zhi Xu Ming-Zhi Xu |
| author_sort | Li-ting Jin |
| collection | DOAJ |
| description | In obese patients, non-alcoholic fatty liver (NAFLD) is common. However, whether there is a connection between the gut microbiota and the onset of NAFLD in obese people is yet unknown. Using quantitative real-time PCR, the microbiota of feces of the eligible 181 obese individuals was identified to compare the differences in gut microbiota between obesity with NAFLD and simple obesity. According to the findings, the gut dominant microbiota was similar between obesity with NAFLD and simple obesity. Nonetheless, compared to the simple obesity group, the quantity of Faecalibacterium prausnitzii colonies was much lower in the obesity with the NAFLD group. Bacteroides were present in greater than 65% of both groups. Bacteroides, Clostridium leptum, and Clostridium butyricum accounted for more than 80% of the cases in the obesity with NAFLD group, whereas Bacteroides, Clostridium butyricum, and F. prausnitzii accounted for more than 80% of the cases in the simple obesity group. We look for potential contributing variables to obesity-related NAFLD and potential prevention measures for obese people. Based on a multi-factor logistic regression analysis, lymphocytes may be a risk factor for obesity with NAFLD while F. prausnitzii may be a protective factor. Additionally, F. prausnitzii is positively impacted by Bacteroides, Clostridium leptum, Clostridium butyricum, and Eubacterium rectale, yet adversely impacted by Enterobacteriaceae. Notably, lymphocytes and F. prausnitzii may help determine whether obese patients would develop NAFLD. |
| first_indexed | 2024-04-10T20:55:56Z |
| format | Article |
| id | doaj.art-b7c7f018932c4935a6c0e4eed4e25f2e |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-10T20:55:56Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-b7c7f018932c4935a6c0e4eed4e25f2e2023-01-23T06:18:40ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-01-011310.3389/fcimb.2023.11136431113643Characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver diseaseLi-ting Jin0Ming-Zhi Xu1Ming-Zhi Xu2Zhejiang University of Medicine, Hangzhou, Zhejiang, ChinaDepartment of General Medicine, Zhejiang Cancer Hospital, Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang, ChinaDepartment of Endocrinology and Metabolic Disease, Shulan (Hangzhou) Hospital Affiliated to Zhejiang Shuren University Shulan International Medical College, Hangzhou, Zhejiang, ChinaIn obese patients, non-alcoholic fatty liver (NAFLD) is common. However, whether there is a connection between the gut microbiota and the onset of NAFLD in obese people is yet unknown. Using quantitative real-time PCR, the microbiota of feces of the eligible 181 obese individuals was identified to compare the differences in gut microbiota between obesity with NAFLD and simple obesity. According to the findings, the gut dominant microbiota was similar between obesity with NAFLD and simple obesity. Nonetheless, compared to the simple obesity group, the quantity of Faecalibacterium prausnitzii colonies was much lower in the obesity with the NAFLD group. Bacteroides were present in greater than 65% of both groups. Bacteroides, Clostridium leptum, and Clostridium butyricum accounted for more than 80% of the cases in the obesity with NAFLD group, whereas Bacteroides, Clostridium butyricum, and F. prausnitzii accounted for more than 80% of the cases in the simple obesity group. We look for potential contributing variables to obesity-related NAFLD and potential prevention measures for obese people. Based on a multi-factor logistic regression analysis, lymphocytes may be a risk factor for obesity with NAFLD while F. prausnitzii may be a protective factor. Additionally, F. prausnitzii is positively impacted by Bacteroides, Clostridium leptum, Clostridium butyricum, and Eubacterium rectale, yet adversely impacted by Enterobacteriaceae. Notably, lymphocytes and F. prausnitzii may help determine whether obese patients would develop NAFLD.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1113643/fullFaecalibacterium prausnitziilymphocytesmicrobiota interactionpathogenic factordisease prevention |
| spellingShingle | Li-ting Jin Ming-Zhi Xu Ming-Zhi Xu Characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver disease Frontiers in Cellular and Infection Microbiology Faecalibacterium prausnitzii lymphocytes microbiota interaction pathogenic factor disease prevention |
| title | Characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver disease |
| title_full | Characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver disease |
| title_fullStr | Characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver disease |
| title_full_unstemmed | Characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver disease |
| title_short | Characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver disease |
| title_sort | characterization of gut dominant microbiota in obese patients with nonalcoholic fatty liver disease |
| topic | Faecalibacterium prausnitzii lymphocytes microbiota interaction pathogenic factor disease prevention |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1113643/full |
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