Role of neurosteroid allopregnanolone on age-related differences in exercise-induced hypoalgesia in rats
The beneficial effects of physical activity for pain are denominated exercise-induced hypoalgesia (EIH). Here, we examined the age-related change and potential role of the neurosteroid allopregnanolone (ALLO) on EIH in rats. Adult and aged rats were randomly divided into one of three groups; non-exe...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-02-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861318302135 |
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| author | Bun Aoyama Takashi Kawano Hideki Iwata Atsushi Nishigaki Daiki Yamanaka Hiroki Tateiwa Marie Shigematsu-Locatelli Satoru Eguchi Fabricio M. Locatelli Masataka Yokoyama |
| author_facet | Bun Aoyama Takashi Kawano Hideki Iwata Atsushi Nishigaki Daiki Yamanaka Hiroki Tateiwa Marie Shigematsu-Locatelli Satoru Eguchi Fabricio M. Locatelli Masataka Yokoyama |
| author_sort | Bun Aoyama |
| collection | DOAJ |
| description | The beneficial effects of physical activity for pain are denominated exercise-induced hypoalgesia (EIH). Here, we examined the age-related change and potential role of the neurosteroid allopregnanolone (ALLO) on EIH in rats. Adult and aged rats were randomly divided into one of three groups; non-exercise control, Low-exercise, and High-exercise. The animals in the Low- and High-exercise groups were subjected to a 10-minute treadmill workout at 40% and 80% maximum oxygen intake intensity, respectively. In the Low-exercise groups, a significant EIH response was observed in aged but not in adult rats. The pre-treatment with ALLO synthesis inhibitor finasteride, but not opioid-receptor antagonist naloxone, inhibited the Low-exercise induced EIH response in aged rats. Furthermore, the Low-exercise increased brain ALLO levels in aged animals compared with controls, which was correlated with the mechanical pain sensitivity. On the other hand, High-exercise could induce EIH response in both adult and aged animals, but it was more effective in adult rats. The pre-treatment with naloxone, but not finasteride, reduced the EIH observed after High-exercise in both adult and aged rats. Our findings demonstrated that effective EIH can be achieved even by mild-intensity exercise in aged animals via an increase of the brain ALLO levels. Keywords: Neurosteroid, Allopregnanolone, Aging, Exercise, Acute pain |
| first_indexed | 2024-12-14T22:34:08Z |
| format | Article |
| id | doaj.art-b7c9e3087e38472292b1f43077bdedd5 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-14T22:34:08Z |
| publishDate | 2019-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-b7c9e3087e38472292b1f43077bdedd52022-12-21T22:45:12ZengElsevierJournal of Pharmacological Sciences1347-86132019-02-0113927783Role of neurosteroid allopregnanolone on age-related differences in exercise-induced hypoalgesia in ratsBun Aoyama0Takashi Kawano1Hideki Iwata2Atsushi Nishigaki3Daiki Yamanaka4Hiroki Tateiwa5Marie Shigematsu-Locatelli6Satoru Eguchi7Fabricio M. Locatelli8Masataka Yokoyama9Department of Anesthesiology and Intensive Care Medicine, Kochi Medical School, Nankoku, Kochi, JapanDepartment of Anesthesiology and Intensive Care Medicine, Kochi Medical School, Nankoku, Kochi, Japan; Corresponding author. Department of Anesthesiology and Intensive Care Medicine, Kochi Medical School, Kohasu, Oko-cho, Nankoku, Kochi 783-8505, Japan. Fax: +81 88 880 2475.Department of Anesthesiology and Intensive Care Medicine, Kochi Medical School, Nankoku, Kochi, JapanDepartment of Anesthesiology and Intensive Care Medicine, Kochi Medical School, Nankoku, Kochi, JapanDepartment of Anesthesiology and Intensive Care Medicine, Kochi Medical School, Nankoku, Kochi, JapanDepartment of Anesthesiology and Intensive Care Medicine, Kochi Medical School, Nankoku, Kochi, JapanDepartment of Anesthesiology and Intensive Care Medicine, Kochi Medical School, Nankoku, Kochi, JapanDepartment of Dental Anesthesiology, Tokushima University School of Dentistry, Tokushima, JapanDepartment of Anesthesiology and Intensive Care Medicine, Kochi Medical School, Nankoku, Kochi, JapanDepartment of Anesthesiology and Intensive Care Medicine, Kochi Medical School, Nankoku, Kochi, JapanThe beneficial effects of physical activity for pain are denominated exercise-induced hypoalgesia (EIH). Here, we examined the age-related change and potential role of the neurosteroid allopregnanolone (ALLO) on EIH in rats. Adult and aged rats were randomly divided into one of three groups; non-exercise control, Low-exercise, and High-exercise. The animals in the Low- and High-exercise groups were subjected to a 10-minute treadmill workout at 40% and 80% maximum oxygen intake intensity, respectively. In the Low-exercise groups, a significant EIH response was observed in aged but not in adult rats. The pre-treatment with ALLO synthesis inhibitor finasteride, but not opioid-receptor antagonist naloxone, inhibited the Low-exercise induced EIH response in aged rats. Furthermore, the Low-exercise increased brain ALLO levels in aged animals compared with controls, which was correlated with the mechanical pain sensitivity. On the other hand, High-exercise could induce EIH response in both adult and aged animals, but it was more effective in adult rats. The pre-treatment with naloxone, but not finasteride, reduced the EIH observed after High-exercise in both adult and aged rats. Our findings demonstrated that effective EIH can be achieved even by mild-intensity exercise in aged animals via an increase of the brain ALLO levels. Keywords: Neurosteroid, Allopregnanolone, Aging, Exercise, Acute painhttp://www.sciencedirect.com/science/article/pii/S1347861318302135 |
| spellingShingle | Bun Aoyama Takashi Kawano Hideki Iwata Atsushi Nishigaki Daiki Yamanaka Hiroki Tateiwa Marie Shigematsu-Locatelli Satoru Eguchi Fabricio M. Locatelli Masataka Yokoyama Role of neurosteroid allopregnanolone on age-related differences in exercise-induced hypoalgesia in rats Journal of Pharmacological Sciences |
| title | Role of neurosteroid allopregnanolone on age-related differences in exercise-induced hypoalgesia in rats |
| title_full | Role of neurosteroid allopregnanolone on age-related differences in exercise-induced hypoalgesia in rats |
| title_fullStr | Role of neurosteroid allopregnanolone on age-related differences in exercise-induced hypoalgesia in rats |
| title_full_unstemmed | Role of neurosteroid allopregnanolone on age-related differences in exercise-induced hypoalgesia in rats |
| title_short | Role of neurosteroid allopregnanolone on age-related differences in exercise-induced hypoalgesia in rats |
| title_sort | role of neurosteroid allopregnanolone on age related differences in exercise induced hypoalgesia in rats |
| url | http://www.sciencedirect.com/science/article/pii/S1347861318302135 |
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