Electrochemical sensors for simultaneous detection of dopamine and uric acid based on a composite of electrochemically reduced graphene oxide and PEDOT:PSS-modified glassy carbon electrode
Herein, we report the development of a successful electrochemical sensor based on a composite of electrochemically reduced graphene oxide (ErGO) and poly(3,4-ethylenedioxythiophene):poly(styrene-4-sulphonate) (PEDOT:PSS) for the simultaneous detection of dopamine (DA) and uric acid (UA). A glassy ca...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715623002631 |
| _version_ | 1797398647718019072 |
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| author | Shafa Aini Hasnawati Ta'alia Eti Rohaeti Budi Riza Putra Wulan Tri Wahyuni |
| author_facet | Shafa Aini Hasnawati Ta'alia Eti Rohaeti Budi Riza Putra Wulan Tri Wahyuni |
| author_sort | Shafa Aini Hasnawati Ta'alia |
| collection | DOAJ |
| description | Herein, we report the development of a successful electrochemical sensor based on a composite of electrochemically reduced graphene oxide (ErGO) and poly(3,4-ethylenedioxythiophene):poly(styrene-4-sulphonate) (PEDOT:PSS) for the simultaneous detection of dopamine (DA) and uric acid (UA). A glassy carbon electrode was modified with a composite of ErGO and PEDOT:PSS and further characterized using Raman spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and electrochemical impedance spectroscopy. The obtained composition of weight ratio between ErGO and PEDOT:PSS as 7:3 demonstrated the highest electrocatalytic activity for the simultaneous detection of DA and UA when it was investigated using differential pulse voltammetry technique under optimized experimental conditions. The developed sensor also exhibited two separate well-defined anodic peaks of both analytes with a linear response over the concentration range of 3–33 µM for DA and 10–110 µM for UA. The limits of detection for DA and UA were 0.4 and 0.5 µM, respectively. The proposed sensor also displayed good stability and reproducibility for the simultaneous detection of DA and UA for five consecutive days of measurements. In addition, the developed sensor exhibited a good selectivity for the simultaneous detection of DA and UA against several potential interferences, such as K+, Cl−, C2O42−, PO43−, ascorbic acid, glucose, and urea, with % recovery values of 97%–101% for DA and 97%–103% for UA, which are acceptable in the analytical range. Furthermore, this sensor was successfully used in detecting DA and UA in real human samples using standard addition techniques and thus may have a potency to be further employed in practical applications. |
| first_indexed | 2024-03-09T01:28:35Z |
| format | Article |
| id | doaj.art-b7cd08e16f204d6cbe5ed732e65c1248 |
| institution | Directory Open Access Journal |
| issn | 2211-7156 |
| language | English |
| last_indexed | 2024-03-09T01:28:35Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj.art-b7cd08e16f204d6cbe5ed732e65c12482023-12-10T06:14:56ZengElsevierResults in Chemistry2211-71562023-12-016101024Electrochemical sensors for simultaneous detection of dopamine and uric acid based on a composite of electrochemically reduced graphene oxide and PEDOT:PSS-modified glassy carbon electrodeShafa Aini Hasnawati Ta'alia0Eti Rohaeti1Budi Riza Putra2Wulan Tri Wahyuni3Analytical Chemistry Division, Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, IndonesiaAnalytical Chemistry Division, Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, IndonesiaResearch Center for Metallurgy, National Research and Innovation Agency (BRIN), PUSPIPTEK Gd. 470, South Tangerang, Banten, IndonesiaAnalytical Chemistry Division, Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Indonesia; Tropical Biopharmaca Research Center, Institute of Research and Community Empowerment, IPB University, Indonesia; Corresponding author at: Analytical Chemistry Division, Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Indonesia.Herein, we report the development of a successful electrochemical sensor based on a composite of electrochemically reduced graphene oxide (ErGO) and poly(3,4-ethylenedioxythiophene):poly(styrene-4-sulphonate) (PEDOT:PSS) for the simultaneous detection of dopamine (DA) and uric acid (UA). A glassy carbon electrode was modified with a composite of ErGO and PEDOT:PSS and further characterized using Raman spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and electrochemical impedance spectroscopy. The obtained composition of weight ratio between ErGO and PEDOT:PSS as 7:3 demonstrated the highest electrocatalytic activity for the simultaneous detection of DA and UA when it was investigated using differential pulse voltammetry technique under optimized experimental conditions. The developed sensor also exhibited two separate well-defined anodic peaks of both analytes with a linear response over the concentration range of 3–33 µM for DA and 10–110 µM for UA. The limits of detection for DA and UA were 0.4 and 0.5 µM, respectively. The proposed sensor also displayed good stability and reproducibility for the simultaneous detection of DA and UA for five consecutive days of measurements. In addition, the developed sensor exhibited a good selectivity for the simultaneous detection of DA and UA against several potential interferences, such as K+, Cl−, C2O42−, PO43−, ascorbic acid, glucose, and urea, with % recovery values of 97%–101% for DA and 97%–103% for UA, which are acceptable in the analytical range. Furthermore, this sensor was successfully used in detecting DA and UA in real human samples using standard addition techniques and thus may have a potency to be further employed in practical applications.http://www.sciencedirect.com/science/article/pii/S2211715623002631Reduced graphene oxidePEDOT:PSSUric acidDopamineSimultaneous detection |
| spellingShingle | Shafa Aini Hasnawati Ta'alia Eti Rohaeti Budi Riza Putra Wulan Tri Wahyuni Electrochemical sensors for simultaneous detection of dopamine and uric acid based on a composite of electrochemically reduced graphene oxide and PEDOT:PSS-modified glassy carbon electrode Results in Chemistry Reduced graphene oxide PEDOT:PSS Uric acid Dopamine Simultaneous detection |
| title | Electrochemical sensors for simultaneous detection of dopamine and uric acid based on a composite of electrochemically reduced graphene oxide and PEDOT:PSS-modified glassy carbon electrode |
| title_full | Electrochemical sensors for simultaneous detection of dopamine and uric acid based on a composite of electrochemically reduced graphene oxide and PEDOT:PSS-modified glassy carbon electrode |
| title_fullStr | Electrochemical sensors for simultaneous detection of dopamine and uric acid based on a composite of electrochemically reduced graphene oxide and PEDOT:PSS-modified glassy carbon electrode |
| title_full_unstemmed | Electrochemical sensors for simultaneous detection of dopamine and uric acid based on a composite of electrochemically reduced graphene oxide and PEDOT:PSS-modified glassy carbon electrode |
| title_short | Electrochemical sensors for simultaneous detection of dopamine and uric acid based on a composite of electrochemically reduced graphene oxide and PEDOT:PSS-modified glassy carbon electrode |
| title_sort | electrochemical sensors for simultaneous detection of dopamine and uric acid based on a composite of electrochemically reduced graphene oxide and pedot pss modified glassy carbon electrode |
| topic | Reduced graphene oxide PEDOT:PSS Uric acid Dopamine Simultaneous detection |
| url | http://www.sciencedirect.com/science/article/pii/S2211715623002631 |
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