sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central Metabolism
In contrast to transcriptional regulation, post-transcriptional regulation and the role of small non-coding RNAs (sRNAs) in streptomycetes are not well studied. Here, we focus on the highly conserved sRNA scr5239 in Streptomyces coelicolor. A proteomics approach revealed that the sRNA regulates seve...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.03121/full |
| _version_ | 1818305645226491904 |
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| author | Franziska Engel Elena Ossipova Per-Johan Jakobsson Michael-Paul Vockenhuber Beatrix Suess |
| author_facet | Franziska Engel Elena Ossipova Per-Johan Jakobsson Michael-Paul Vockenhuber Beatrix Suess |
| author_sort | Franziska Engel |
| collection | DOAJ |
| description | In contrast to transcriptional regulation, post-transcriptional regulation and the role of small non-coding RNAs (sRNAs) in streptomycetes are not well studied. Here, we focus on the highly conserved sRNA scr5239 in Streptomyces coelicolor. A proteomics approach revealed that the sRNA regulates several metabolic enzymes, among them phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme of the central carbon metabolism. The sRNA scr5239 represses pepck at the post-transcriptional level and thus modulates the intracellular level of phosphoenolpyruvate (PEP). The expression of scr5239 in turn is dependent on the global transcriptional regulator DasR, thus creating a feedback loop regulation of the central carbon metabolism. By post-transcriptional regulation of PEPCK and in all likelihood other targets, scr5239 adds an additional layer to the DasR regulatory network and provides a tool to control the metabolism dependent on the available carbon source. |
| first_indexed | 2024-12-13T06:29:53Z |
| format | Article |
| id | doaj.art-b7cf6e50c8044d57bbf4d6367542062f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T06:29:53Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-b7cf6e50c8044d57bbf4d6367542062f2022-12-21T23:56:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-01-011010.3389/fmicb.2019.03121500702sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central MetabolismFranziska Engel0Elena Ossipova1Per-Johan Jakobsson2Michael-Paul Vockenhuber3Beatrix Suess4Synthetic Genetic Circuits, Department of Biology, Darmstadt University Technology, Darmstadt, GermanyDivision of Rheumatology, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Solna, SwedenDivision of Rheumatology, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Solna, SwedenSynthetic Genetic Circuits, Department of Biology, Darmstadt University Technology, Darmstadt, GermanySynthetic Genetic Circuits, Department of Biology, Darmstadt University Technology, Darmstadt, GermanyIn contrast to transcriptional regulation, post-transcriptional regulation and the role of small non-coding RNAs (sRNAs) in streptomycetes are not well studied. Here, we focus on the highly conserved sRNA scr5239 in Streptomyces coelicolor. A proteomics approach revealed that the sRNA regulates several metabolic enzymes, among them phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme of the central carbon metabolism. The sRNA scr5239 represses pepck at the post-transcriptional level and thus modulates the intracellular level of phosphoenolpyruvate (PEP). The expression of scr5239 in turn is dependent on the global transcriptional regulator DasR, thus creating a feedback loop regulation of the central carbon metabolism. By post-transcriptional regulation of PEPCK and in all likelihood other targets, scr5239 adds an additional layer to the DasR regulatory network and provides a tool to control the metabolism dependent on the available carbon source.https://www.frontiersin.org/article/10.3389/fmicb.2019.03121/fullsRNAStreptomycesDasRcarbon metabolismphosphoenolpyruvatePEPCK |
| spellingShingle | Franziska Engel Elena Ossipova Per-Johan Jakobsson Michael-Paul Vockenhuber Beatrix Suess sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central Metabolism Frontiers in Microbiology sRNA Streptomyces DasR carbon metabolism phosphoenolpyruvate PEPCK |
| title | sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central Metabolism |
| title_full | sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central Metabolism |
| title_fullStr | sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central Metabolism |
| title_full_unstemmed | sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central Metabolism |
| title_short | sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central Metabolism |
| title_sort | srna scr5239 involved in feedback loop regulation of streptomyces coelicolor central metabolism |
| topic | sRNA Streptomyces DasR carbon metabolism phosphoenolpyruvate PEPCK |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.03121/full |
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