The effect of drinking milk containing conjugated linoleic acid on fecal microbiological profile, enzymatic activity, and fecal characteristics in humans
<p>Abstract</p> <p>Background</p> <p>The primary objective was to determine whether consumption of conjugated linoleic acids (CLAs) affected the fecal microbiota composition, fecal enzyme activity or fecal composition.</p> <p>Methods</p> <p>Human...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-07-01
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| Series: | Nutrition Journal |
| Online Access: | http://www.nutritionj.com/content/6/1/15 |
| _version_ | 1818035854276296704 |
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| author | Maf Akier A Venkatramanan Sudha Jacques Helene Chouinard Yvan P Farnworth Edward R Defnoun Sabrina Jones Peter JH |
| author_facet | Maf Akier A Venkatramanan Sudha Jacques Helene Chouinard Yvan P Farnworth Edward R Defnoun Sabrina Jones Peter JH |
| author_sort | Maf Akier A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The primary objective was to determine whether consumption of conjugated linoleic acids (CLAs) affected the fecal microbiota composition, fecal enzyme activity or fecal composition.</p> <p>Methods</p> <p>Human subjects consumed (1 <it>L</it>/day) cows' milk (4% fat) containing (5 mg/g fat) <it>cis</it>-9, <it>trans</it>-11 CLA (CONT), (32 mg/g fat) cis-9, <it>trans</it>-11 CLA (NAT) and (32 mg/g fat) <it>trans</it>-10, <it>cis</it>-12 CLA and <it>cis</it>-9, <it>trans</it>-11 CLA (SYN) for 8 weeks, in addition to their normal diet. Milk feeding periods were separated by 4 week washout periods. Fecal samples were obtained at the beginning (day 0) and the end (day 56) of each milk feeding period. Fecal samples were analysed for microbiological profile, enzyme activity, pH and short chain fatty acid content.</p> <p>Results</p> <p>Samples taken at day 0 and day 56 indicated that the numbers of <it>lactobacilli </it>and <it>bifidobacteria </it>significantly decreased after consumption of all experimental milks; total aerobes, total anaerobes, enterobacteria, and enterococci + streptococci did not change. At day 56, the activities of β-glucosidase, nitroreductase, and urease enzymes had decreased compared to samples taken on day 0 for all treatments. β-glucuronidase activity did not change. Fecal pH and ammonia content did not change.</p> <p>Conclusion</p> <p>It was concluded that observed changes could have been attributed to increased milk intake; no differences could be attributed to consumption of the different CLAs.</p> |
| first_indexed | 2024-12-10T07:01:40Z |
| format | Article |
| id | doaj.art-b7d1562a0f6e4dbeb69eba21fbf94e7e |
| institution | Directory Open Access Journal |
| issn | 1475-2891 |
| language | English |
| last_indexed | 2024-12-10T07:01:40Z |
| publishDate | 2007-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition Journal |
| spelling | doaj.art-b7d1562a0f6e4dbeb69eba21fbf94e7e2022-12-22T01:58:19ZengBMCNutrition Journal1475-28912007-07-01611510.1186/1475-2891-6-15The effect of drinking milk containing conjugated linoleic acid on fecal microbiological profile, enzymatic activity, and fecal characteristics in humansMaf Akier AVenkatramanan SudhaJacques HeleneChouinard Yvan PFarnworth Edward RDefnoun SabrinaJones Peter JH<p>Abstract</p> <p>Background</p> <p>The primary objective was to determine whether consumption of conjugated linoleic acids (CLAs) affected the fecal microbiota composition, fecal enzyme activity or fecal composition.</p> <p>Methods</p> <p>Human subjects consumed (1 <it>L</it>/day) cows' milk (4% fat) containing (5 mg/g fat) <it>cis</it>-9, <it>trans</it>-11 CLA (CONT), (32 mg/g fat) cis-9, <it>trans</it>-11 CLA (NAT) and (32 mg/g fat) <it>trans</it>-10, <it>cis</it>-12 CLA and <it>cis</it>-9, <it>trans</it>-11 CLA (SYN) for 8 weeks, in addition to their normal diet. Milk feeding periods were separated by 4 week washout periods. Fecal samples were obtained at the beginning (day 0) and the end (day 56) of each milk feeding period. Fecal samples were analysed for microbiological profile, enzyme activity, pH and short chain fatty acid content.</p> <p>Results</p> <p>Samples taken at day 0 and day 56 indicated that the numbers of <it>lactobacilli </it>and <it>bifidobacteria </it>significantly decreased after consumption of all experimental milks; total aerobes, total anaerobes, enterobacteria, and enterococci + streptococci did not change. At day 56, the activities of β-glucosidase, nitroreductase, and urease enzymes had decreased compared to samples taken on day 0 for all treatments. β-glucuronidase activity did not change. Fecal pH and ammonia content did not change.</p> <p>Conclusion</p> <p>It was concluded that observed changes could have been attributed to increased milk intake; no differences could be attributed to consumption of the different CLAs.</p>http://www.nutritionj.com/content/6/1/15 |
| spellingShingle | Maf Akier A Venkatramanan Sudha Jacques Helene Chouinard Yvan P Farnworth Edward R Defnoun Sabrina Jones Peter JH The effect of drinking milk containing conjugated linoleic acid on fecal microbiological profile, enzymatic activity, and fecal characteristics in humans Nutrition Journal |
| title | The effect of drinking milk containing conjugated linoleic acid on fecal microbiological profile, enzymatic activity, and fecal characteristics in humans |
| title_full | The effect of drinking milk containing conjugated linoleic acid on fecal microbiological profile, enzymatic activity, and fecal characteristics in humans |
| title_fullStr | The effect of drinking milk containing conjugated linoleic acid on fecal microbiological profile, enzymatic activity, and fecal characteristics in humans |
| title_full_unstemmed | The effect of drinking milk containing conjugated linoleic acid on fecal microbiological profile, enzymatic activity, and fecal characteristics in humans |
| title_short | The effect of drinking milk containing conjugated linoleic acid on fecal microbiological profile, enzymatic activity, and fecal characteristics in humans |
| title_sort | effect of drinking milk containing conjugated linoleic acid on fecal microbiological profile enzymatic activity and fecal characteristics in humans |
| url | http://www.nutritionj.com/content/6/1/15 |
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