Proteomics Research Reveals the Molecular Mechanism by Which Grape Seed Oil Inhibits Tuber Sprouting in Potato
Potato tubers are rich in starch, vitamins, protein, minerals, and other nutrients. However, tuber sprouting produces solanine and reduces the commodity value of potatoes during storage. At present, it is known that some plant essential oils can inhibit tuber sprouting. It has been reported that gra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/8/890 |
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| author | Chengcheng Lyu Xing Zhang Xiang Li Yifei Lu Jichao Yuan Liming Lu Qiang Wang Xiyao Wang Liqin Li |
| author_facet | Chengcheng Lyu Xing Zhang Xiang Li Yifei Lu Jichao Yuan Liming Lu Qiang Wang Xiyao Wang Liqin Li |
| author_sort | Chengcheng Lyu |
| collection | DOAJ |
| description | Potato tubers are rich in starch, vitamins, protein, minerals, and other nutrients. However, tuber sprouting produces solanine and reduces the commodity value of potatoes during storage. At present, it is known that some plant essential oils can inhibit tuber sprouting. It has been reported that grape seed oil (GSO) has antioxidant, anti-inflammatory, and anticancer characteristics, reducing blood lipids and delaying aging. In this study, we found for the first time that GSO delayed tuber sprouting, and the soluble sugar content and peroxidase activity changed after 60 days of GSO treatment. Furthermore, a comparative proteomic analysis of tuber bud eyes showed that after 30 days of GSO treatment, there were 206 and 129 differentially abundant proteins (DAPs) with increased and decreased abundance levels, respectively. After analysis, we found that 15 ROS-related proteins and 14 proteins involved in energy metabolism were DAPs. Among them, gamma aminobutyrate transaminase 1 had decreased abundance after GSO treatment. Meanwhile, the transcription level of genes related to GABA synthesis increased significantly according to qRT-PCR analysis. Our results provide new approaches to the proteomic mechanism of potato sprouting after GSO treatment and provide a theoretical basis for the application of GSO in inhibiting potato seed sprouts. |
| first_indexed | 2024-03-10T23:53:39Z |
| format | Article |
| id | doaj.art-b7d330c61f2641f6b7aa4a6336593094 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T23:53:39Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-b7d330c61f2641f6b7aa4a63365930942023-11-19T01:21:16ZengMDPI AGHorticulturae2311-75242023-08-019889010.3390/horticulturae9080890Proteomics Research Reveals the Molecular Mechanism by Which Grape Seed Oil Inhibits Tuber Sprouting in PotatoChengcheng Lyu0Xing Zhang1Xiang Li2Yifei Lu3Jichao Yuan4Liming Lu5Qiang Wang6Xiyao Wang7Liqin Li8State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agriculture University, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agriculture University, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agriculture University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaPotato tubers are rich in starch, vitamins, protein, minerals, and other nutrients. However, tuber sprouting produces solanine and reduces the commodity value of potatoes during storage. At present, it is known that some plant essential oils can inhibit tuber sprouting. It has been reported that grape seed oil (GSO) has antioxidant, anti-inflammatory, and anticancer characteristics, reducing blood lipids and delaying aging. In this study, we found for the first time that GSO delayed tuber sprouting, and the soluble sugar content and peroxidase activity changed after 60 days of GSO treatment. Furthermore, a comparative proteomic analysis of tuber bud eyes showed that after 30 days of GSO treatment, there were 206 and 129 differentially abundant proteins (DAPs) with increased and decreased abundance levels, respectively. After analysis, we found that 15 ROS-related proteins and 14 proteins involved in energy metabolism were DAPs. Among them, gamma aminobutyrate transaminase 1 had decreased abundance after GSO treatment. Meanwhile, the transcription level of genes related to GABA synthesis increased significantly according to qRT-PCR analysis. Our results provide new approaches to the proteomic mechanism of potato sprouting after GSO treatment and provide a theoretical basis for the application of GSO in inhibiting potato seed sprouts.https://www.mdpi.com/2311-7524/9/8/890potatotubergrape seed oilsproutingproteomics |
| spellingShingle | Chengcheng Lyu Xing Zhang Xiang Li Yifei Lu Jichao Yuan Liming Lu Qiang Wang Xiyao Wang Liqin Li Proteomics Research Reveals the Molecular Mechanism by Which Grape Seed Oil Inhibits Tuber Sprouting in Potato Horticulturae potato tuber grape seed oil sprouting proteomics |
| title | Proteomics Research Reveals the Molecular Mechanism by Which Grape Seed Oil Inhibits Tuber Sprouting in Potato |
| title_full | Proteomics Research Reveals the Molecular Mechanism by Which Grape Seed Oil Inhibits Tuber Sprouting in Potato |
| title_fullStr | Proteomics Research Reveals the Molecular Mechanism by Which Grape Seed Oil Inhibits Tuber Sprouting in Potato |
| title_full_unstemmed | Proteomics Research Reveals the Molecular Mechanism by Which Grape Seed Oil Inhibits Tuber Sprouting in Potato |
| title_short | Proteomics Research Reveals the Molecular Mechanism by Which Grape Seed Oil Inhibits Tuber Sprouting in Potato |
| title_sort | proteomics research reveals the molecular mechanism by which grape seed oil inhibits tuber sprouting in potato |
| topic | potato tuber grape seed oil sprouting proteomics |
| url | https://www.mdpi.com/2311-7524/9/8/890 |
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