The Effects of Acute and Chronic Aerobic Activity on the Signaling Pathway of the Inflammasome NLRP3 Complex in Young Men
<i>Background and Objectives</i>: The results of the studies show that the intensity and volume of aerobic exercise activity produce different responses of the immune system. This study aims to show how the signaling pathway of the inflammatory NLRP3 complex is influenced by the acute an...
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| Format: | Article |
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MDPI AG
2019-04-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1010-660X/55/4/105 |
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| author | Iman Khakroo Abkenar Farhad Rahmani-nia Giovanni Lombardi |
| author_facet | Iman Khakroo Abkenar Farhad Rahmani-nia Giovanni Lombardi |
| author_sort | Iman Khakroo Abkenar |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: The results of the studies show that the intensity and volume of aerobic exercise activity produce different responses of the immune system. This study aims to show how the signaling pathway of the inflammatory NLRP3 complex is influenced by the acute and chronic effects of moderate and high-intensity aerobic exercises in young men. <i>Materials and Methods</i>: Accordingly, 60 healthy (BMI = 23.56 ± 2.67) young (24.4 ± 0.4) students volunteered to participate in the study that was randomly divided into two experimental (<i>n</i> = 20) groups and one control (<i>n</i> = 20) group. The training protocol started with two intensity levels of 50% for a moderate group and 70% of maximum heart rate for high group for 30 min and then continued until reaching 70% (moderate group) and 90% (high group) of the maximum heart rate, respectively. Using Real Time-PCR method, the expression of NLRP3 gene and ELISA- were measured by IL-1β, IL-18. <i>Results</i>: The results showed that acute aerobic exercise with moderate intensity had no significant effect on the expression of NLRP3 gene and serum levels of IL-1β and IL-18 cytokines (<i>p</i> > 0.05) when acute exercise, with high intensity, begins an initiation of the activity of the inflammatory complex with elevated serum levels of IL-1β, IL-18, and NLRP3 gene expression (<i>p</i> < 0.05). In addition, chronic exercise with moderate intensity significantly reduced the expression of NLRP3 gene and serum levels of IL-1β, IL-18 cytokines (<i>p</i> < 0.05). In the case of chronic exercise with high intensity, a significant increase in expression of gene, NLRP3 and serum levels of IL-1β, IL-18 cytokines were observed (<i>p</i> < 0.05). <i>Conclusions</i>: Generally, it can be concluded that chronic exercise with moderate intensity is effective in decreasing the expression of the inflammasome and inflammation. |
| first_indexed | 2024-03-12T11:08:52Z |
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| language | English |
| last_indexed | 2024-03-12T11:08:52Z |
| publishDate | 2019-04-01 |
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| series | Medicina |
| spelling | doaj.art-b7d633b95b3545d3ada630d6ee6670922023-09-02T03:15:42ZengMDPI AGMedicina1010-660X2019-04-0155410510.3390/medicina55040105medicina55040105The Effects of Acute and Chronic Aerobic Activity on the Signaling Pathway of the Inflammasome NLRP3 Complex in Young MenIman Khakroo Abkenar0Farhad Rahmani-nia1Giovanni Lombardi2Department of Exercise Physiology, Faculty of Sport Sciences, University of Guilan, Rasht 4199613776, IranDepartment of Exercise Physiology, Faculty of Sport Sciences, University of Guilan, Rasht 4199613776, IranIRCCS Istituto Ortopedico Galeazzi, Laboratory of Experimental Biochemistry and Molecular Biology, via Riccardo Galeazzi 4, 20161 Milano, Italia<i>Background and Objectives</i>: The results of the studies show that the intensity and volume of aerobic exercise activity produce different responses of the immune system. This study aims to show how the signaling pathway of the inflammatory NLRP3 complex is influenced by the acute and chronic effects of moderate and high-intensity aerobic exercises in young men. <i>Materials and Methods</i>: Accordingly, 60 healthy (BMI = 23.56 ± 2.67) young (24.4 ± 0.4) students volunteered to participate in the study that was randomly divided into two experimental (<i>n</i> = 20) groups and one control (<i>n</i> = 20) group. The training protocol started with two intensity levels of 50% for a moderate group and 70% of maximum heart rate for high group for 30 min and then continued until reaching 70% (moderate group) and 90% (high group) of the maximum heart rate, respectively. Using Real Time-PCR method, the expression of NLRP3 gene and ELISA- were measured by IL-1β, IL-18. <i>Results</i>: The results showed that acute aerobic exercise with moderate intensity had no significant effect on the expression of NLRP3 gene and serum levels of IL-1β and IL-18 cytokines (<i>p</i> > 0.05) when acute exercise, with high intensity, begins an initiation of the activity of the inflammatory complex with elevated serum levels of IL-1β, IL-18, and NLRP3 gene expression (<i>p</i> < 0.05). In addition, chronic exercise with moderate intensity significantly reduced the expression of NLRP3 gene and serum levels of IL-1β, IL-18 cytokines (<i>p</i> < 0.05). In the case of chronic exercise with high intensity, a significant increase in expression of gene, NLRP3 and serum levels of IL-1β, IL-18 cytokines were observed (<i>p</i> < 0.05). <i>Conclusions</i>: Generally, it can be concluded that chronic exercise with moderate intensity is effective in decreasing the expression of the inflammasome and inflammation.https://www.mdpi.com/1010-660X/55/4/105acute and chronic aerobic exerciseinflammationinflammasome complex |
| spellingShingle | Iman Khakroo Abkenar Farhad Rahmani-nia Giovanni Lombardi The Effects of Acute and Chronic Aerobic Activity on the Signaling Pathway of the Inflammasome NLRP3 Complex in Young Men Medicina acute and chronic aerobic exercise inflammation inflammasome complex |
| title | The Effects of Acute and Chronic Aerobic Activity on the Signaling Pathway of the Inflammasome NLRP3 Complex in Young Men |
| title_full | The Effects of Acute and Chronic Aerobic Activity on the Signaling Pathway of the Inflammasome NLRP3 Complex in Young Men |
| title_fullStr | The Effects of Acute and Chronic Aerobic Activity on the Signaling Pathway of the Inflammasome NLRP3 Complex in Young Men |
| title_full_unstemmed | The Effects of Acute and Chronic Aerobic Activity on the Signaling Pathway of the Inflammasome NLRP3 Complex in Young Men |
| title_short | The Effects of Acute and Chronic Aerobic Activity on the Signaling Pathway of the Inflammasome NLRP3 Complex in Young Men |
| title_sort | effects of acute and chronic aerobic activity on the signaling pathway of the inflammasome nlrp3 complex in young men |
| topic | acute and chronic aerobic exercise inflammation inflammasome complex |
| url | https://www.mdpi.com/1010-660X/55/4/105 |
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