Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of <i>Schistosoma japonicum</i> and Schistosomiasis Japonica
The prevalence and infectious intensity of schistosomiasis japonica has decreased significantly in China in the past few decades. However, more accurate and sensitive diagnostic methods are urgently required for the further control, surveillance, and final elimination of the disease. In this study,...
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/1059 |
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| author | Yang Hong Qinghong Guo Xue Zhou Liying Tang Cheng Chen Zheng Shang Kerou Zhou Zhizhong Zhang Jinming Liu Jiaojiao Lin Bin Xu Jun-Hu Chen Zhiqiang Fu Wei Hu |
| author_facet | Yang Hong Qinghong Guo Xue Zhou Liying Tang Cheng Chen Zheng Shang Kerou Zhou Zhizhong Zhang Jinming Liu Jiaojiao Lin Bin Xu Jun-Hu Chen Zhiqiang Fu Wei Hu |
| author_sort | Yang Hong |
| collection | DOAJ |
| description | The prevalence and infectious intensity of schistosomiasis japonica has decreased significantly in China in the past few decades. However, more accurate and sensitive diagnostic methods are urgently required for the further control, surveillance, and final elimination of the disease. In this study, we assessed the diagnostic efficacy of a real-time fluorescence quantitative PCR (qPCR) method and recombinase polymerase amplification (RPA) combined with a lateral-flow dipstick (LFD) assay for detecting early infections of <i>Schistosoma japonicum</i> and different infection intensities. The sensitivity of the qPCR at 40 days post-infection (dpi) was 100% (8/8) in mice infected with 40 cercariae, which was higher than in mice infected with 10 cercariae (90%, 9/10) or five cercariae (77.8%, 7/9). The results of the RPA–LFD assays were similar, with sensitivities of 55.6% (5/9), 80% (8/10), and 100% (8/8) in mice infected with 5, 10, and 40 cercariae, respectively. In goats, both the qPCR and RPA–LFD assays showed 100% (8/8) sensitivity at 56 dpi. In the early detection of <i>S. japonicum</i> infection in mice and goats with qPCR, the first peak in positivity appeared at 3–4 dpi, when the positivity rate exceeded 40%, even in the low infection, intensity mice. In the RPA–LFD assays, positive results first peaked at 4–5 dpi in the mice, and the positivity rate was 37.5% in the goats at 1 dpi. In conclusion, neither of the molecular methods produced exceptional results for the early diagnosis of <i>S. japonicum</i> infection. However, they were useful methods for the regular diagnosis of schistosomiasis in mice and goats. |
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| language | English |
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| publishDate | 2023-04-01 |
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| series | Microorganisms |
| spelling | doaj.art-b7d71fc681f34b6b9d54ea9e0eddecb32023-11-17T20:34:25ZengMDPI AGMicroorganisms2076-26072023-04-01114105910.3390/microorganisms11041059Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of <i>Schistosoma japonicum</i> and Schistosomiasis JaponicaYang Hong0Qinghong Guo1Xue Zhou2Liying Tang3Cheng Chen4Zheng Shang5Kerou Zhou6Zhizhong Zhang7Jinming Liu8Jiaojiao Lin9Bin Xu10Jun-Hu Chen11Zhiqiang Fu12Wei Hu13National Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention (Chinese Center for Tropical Diseases Research), Key Laboratory of Parasite and Vector Biology, National Health Commission of the People’s Republic of China (NHC), World Health Organization (WHO) Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, ChinaNational Reference Laboratory for Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaNational Reference Laboratory for Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaNational Reference Laboratory for Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaNational Reference Laboratory for Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaNational Reference Laboratory for Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaNational Reference Laboratory for Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaNational Reference Laboratory for Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaNational Reference Laboratory for Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaNational Reference Laboratory for Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaNational Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention (Chinese Center for Tropical Diseases Research), Key Laboratory of Parasite and Vector Biology, National Health Commission of the People’s Republic of China (NHC), World Health Organization (WHO) Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, ChinaNational Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention (Chinese Center for Tropical Diseases Research), Key Laboratory of Parasite and Vector Biology, National Health Commission of the People’s Republic of China (NHC), World Health Organization (WHO) Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, ChinaNational Reference Laboratory for Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaNational Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention (Chinese Center for Tropical Diseases Research), Key Laboratory of Parasite and Vector Biology, National Health Commission of the People’s Republic of China (NHC), World Health Organization (WHO) Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, ChinaThe prevalence and infectious intensity of schistosomiasis japonica has decreased significantly in China in the past few decades. However, more accurate and sensitive diagnostic methods are urgently required for the further control, surveillance, and final elimination of the disease. In this study, we assessed the diagnostic efficacy of a real-time fluorescence quantitative PCR (qPCR) method and recombinase polymerase amplification (RPA) combined with a lateral-flow dipstick (LFD) assay for detecting early infections of <i>Schistosoma japonicum</i> and different infection intensities. The sensitivity of the qPCR at 40 days post-infection (dpi) was 100% (8/8) in mice infected with 40 cercariae, which was higher than in mice infected with 10 cercariae (90%, 9/10) or five cercariae (77.8%, 7/9). The results of the RPA–LFD assays were similar, with sensitivities of 55.6% (5/9), 80% (8/10), and 100% (8/8) in mice infected with 5, 10, and 40 cercariae, respectively. In goats, both the qPCR and RPA–LFD assays showed 100% (8/8) sensitivity at 56 dpi. In the early detection of <i>S. japonicum</i> infection in mice and goats with qPCR, the first peak in positivity appeared at 3–4 dpi, when the positivity rate exceeded 40%, even in the low infection, intensity mice. In the RPA–LFD assays, positive results first peaked at 4–5 dpi in the mice, and the positivity rate was 37.5% in the goats at 1 dpi. In conclusion, neither of the molecular methods produced exceptional results for the early diagnosis of <i>S. japonicum</i> infection. However, they were useful methods for the regular diagnosis of schistosomiasis in mice and goats.https://www.mdpi.com/2076-2607/11/4/1059<i>Schistosoma japonicum</i>qPCRRPA–LFDearly infectiondiagnosis |
| spellingShingle | Yang Hong Qinghong Guo Xue Zhou Liying Tang Cheng Chen Zheng Shang Kerou Zhou Zhizhong Zhang Jinming Liu Jiaojiao Lin Bin Xu Jun-Hu Chen Zhiqiang Fu Wei Hu Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of <i>Schistosoma japonicum</i> and Schistosomiasis Japonica Microorganisms <i>Schistosoma japonicum</i> qPCR RPA–LFD early infection diagnosis |
| title | Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of <i>Schistosoma japonicum</i> and Schistosomiasis Japonica |
| title_full | Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of <i>Schistosoma japonicum</i> and Schistosomiasis Japonica |
| title_fullStr | Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of <i>Schistosoma japonicum</i> and Schistosomiasis Japonica |
| title_full_unstemmed | Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of <i>Schistosoma japonicum</i> and Schistosomiasis Japonica |
| title_short | Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection of <i>Schistosoma japonicum</i> and Schistosomiasis Japonica |
| title_sort | two molecular plasma based diagnostic methods to evaluate early infection of i schistosoma japonicum i and schistosomiasis japonica |
| topic | <i>Schistosoma japonicum</i> qPCR RPA–LFD early infection diagnosis |
| url | https://www.mdpi.com/2076-2607/11/4/1059 |
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