Doxycycline induces dysbiosis in female C57BL/6NCrl mice
Abstract Objective This study aims to demonstrate the effect of oral doxycycline on fecal microbiota of mice. Doxycycline is a common effector for control of gene expression using the tet-inducible system in transgenic mice. The effect of oral doxycycline on murine gut microbiota has not been report...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-017-2960-7 |
| _version_ | 1811269407343640576 |
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| author | Felicia D. Duke Boynton Aaron C. Ericsson Mayu Uchihashi Misha L. Dunbar J. Erby Wilkinson |
| author_facet | Felicia D. Duke Boynton Aaron C. Ericsson Mayu Uchihashi Misha L. Dunbar J. Erby Wilkinson |
| author_sort | Felicia D. Duke Boynton |
| collection | DOAJ |
| description | Abstract Objective This study aims to demonstrate the effect of oral doxycycline on fecal microbiota of mice. Doxycycline is a common effector for control of gene expression using the tet-inducible system in transgenic mice. The effect of oral doxycycline on murine gut microbiota has not been reported. We evaluated the effect of doxycycline treatment by sequencing the V4 hypervariable region of the 16S rRNA gene from fecal samples collected during a 4 week course of treatment at a dose of 2 mg/ml in the drinking water. Results The fecal microbiota of treated animals were distinct from control animals; the decreased richness and diversity were characterized primarily by Bacteroides sp. enrichment. These effects persisted when the treatment was temporarily discontinued for 1 week. These data suggest that doxycycline treatment can induce significant dysbiosis, and its effects should be considered when used in animal models that are or maybe sensitive to perturbation of the gut microbiota. |
| first_indexed | 2024-04-12T21:41:30Z |
| format | Article |
| id | doaj.art-b7d77befc050458b9e57a7d2beda8320 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-04-12T21:41:30Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-b7d77befc050458b9e57a7d2beda83202022-12-22T03:15:46ZengBMCBMC Research Notes1756-05002017-11-011011710.1186/s13104-017-2960-7Doxycycline induces dysbiosis in female C57BL/6NCrl miceFelicia D. Duke Boynton0Aaron C. Ericsson1Mayu Uchihashi2Misha L. Dunbar3J. Erby Wilkinson4Unit for Laboratory Animal Medicine, University of Michigan Medical SchoolUniversity of Missouri Metagenomics Research Center, University of MissouriUnit for Laboratory Animal Medicine, University of Michigan Medical SchoolUnit for Laboratory Animal Medicine, University of Michigan Medical SchoolUnit for Laboratory Animal Medicine, University of Michigan Medical SchoolAbstract Objective This study aims to demonstrate the effect of oral doxycycline on fecal microbiota of mice. Doxycycline is a common effector for control of gene expression using the tet-inducible system in transgenic mice. The effect of oral doxycycline on murine gut microbiota has not been reported. We evaluated the effect of doxycycline treatment by sequencing the V4 hypervariable region of the 16S rRNA gene from fecal samples collected during a 4 week course of treatment at a dose of 2 mg/ml in the drinking water. Results The fecal microbiota of treated animals were distinct from control animals; the decreased richness and diversity were characterized primarily by Bacteroides sp. enrichment. These effects persisted when the treatment was temporarily discontinued for 1 week. These data suggest that doxycycline treatment can induce significant dysbiosis, and its effects should be considered when used in animal models that are or maybe sensitive to perturbation of the gut microbiota.http://link.springer.com/article/10.1186/s13104-017-2960-7DoxycyclineTet-onTet-offMicrobiomeMicrobiotaMouse |
| spellingShingle | Felicia D. Duke Boynton Aaron C. Ericsson Mayu Uchihashi Misha L. Dunbar J. Erby Wilkinson Doxycycline induces dysbiosis in female C57BL/6NCrl mice BMC Research Notes Doxycycline Tet-on Tet-off Microbiome Microbiota Mouse |
| title | Doxycycline induces dysbiosis in female C57BL/6NCrl mice |
| title_full | Doxycycline induces dysbiosis in female C57BL/6NCrl mice |
| title_fullStr | Doxycycline induces dysbiosis in female C57BL/6NCrl mice |
| title_full_unstemmed | Doxycycline induces dysbiosis in female C57BL/6NCrl mice |
| title_short | Doxycycline induces dysbiosis in female C57BL/6NCrl mice |
| title_sort | doxycycline induces dysbiosis in female c57bl 6ncrl mice |
| topic | Doxycycline Tet-on Tet-off Microbiome Microbiota Mouse |
| url | http://link.springer.com/article/10.1186/s13104-017-2960-7 |
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