Adipose Tissue: An Emerging Target for Adeno-associated Viral Vectors
Adipose tissue is one of the largest organs, playing important roles in physiology and pathologies of multiple diseases. However, research related to adeno-associated virus (AAV) targeting adipose tissue has been left far behind studies carried out in the liver, brain, heart, and muscle. Despite ini...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Molecular Therapy: Methods & Clinical Development |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2329050120301947 |
| _version_ | 1818856934302810112 |
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| author | Rhiannon Bates Wei Huang Lei Cao |
| author_facet | Rhiannon Bates Wei Huang Lei Cao |
| author_sort | Rhiannon Bates |
| collection | DOAJ |
| description | Adipose tissue is one of the largest organs, playing important roles in physiology and pathologies of multiple diseases. However, research related to adeno-associated virus (AAV) targeting adipose tissue has been left far behind studies carried out in the liver, brain, heart, and muscle. Despite initial reports indicating poor performance, AAV-mediated gene delivery to adipose tissue has continued to rise during the past two decades. AAV8 and a novel engineered hybrid serotype, Rec2, have been shown to transduce adipose tissue more efficiently than other serotypes so far tested and have been applied in most of the in vivo studies. The Rec2 serotype displays high efficacy of gene transfer to both brown and white fat via local and systemic administration. This review summarizes the advances in developing AAV vectors with enhanced adipose tropism and restricting off-target transgene expression. We discuss the challenges and strategies to search for and generate novel serotypes with tropism tailoring for adipose tissue and develop AAV vector systems to improve adipose transgene expression for basic research and translational studies. |
| first_indexed | 2024-12-19T08:32:23Z |
| format | Article |
| id | doaj.art-b7d8105fef524878865d5b47faffee75 |
| institution | Directory Open Access Journal |
| issn | 2329-0501 |
| language | English |
| last_indexed | 2024-12-19T08:32:23Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Methods & Clinical Development |
| spelling | doaj.art-b7d8105fef524878865d5b47faffee752022-12-21T20:29:10ZengElsevierMolecular Therapy: Methods & Clinical Development2329-05012020-12-0119236249Adipose Tissue: An Emerging Target for Adeno-associated Viral VectorsRhiannon Bates0Wei Huang1Lei Cao2Department of Cancer Biology & Genetics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA; The Ohio State University Comprehensive Cancer Center, Columbus, OH 43210, USADepartment of Cancer Biology & Genetics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA; The Ohio State University Comprehensive Cancer Center, Columbus, OH 43210, USADepartment of Cancer Biology & Genetics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA; The Ohio State University Comprehensive Cancer Center, Columbus, OH 43210, USA; Corresponding author: Lei Cao, Department of Cancer Biology & Genetics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.Adipose tissue is one of the largest organs, playing important roles in physiology and pathologies of multiple diseases. However, research related to adeno-associated virus (AAV) targeting adipose tissue has been left far behind studies carried out in the liver, brain, heart, and muscle. Despite initial reports indicating poor performance, AAV-mediated gene delivery to adipose tissue has continued to rise during the past two decades. AAV8 and a novel engineered hybrid serotype, Rec2, have been shown to transduce adipose tissue more efficiently than other serotypes so far tested and have been applied in most of the in vivo studies. The Rec2 serotype displays high efficacy of gene transfer to both brown and white fat via local and systemic administration. This review summarizes the advances in developing AAV vectors with enhanced adipose tropism and restricting off-target transgene expression. We discuss the challenges and strategies to search for and generate novel serotypes with tropism tailoring for adipose tissue and develop AAV vector systems to improve adipose transgene expression for basic research and translational studies.http://www.sciencedirect.com/science/article/pii/S2329050120301947AAVadiposegene therapytissue tropismcapsid engineering |
| spellingShingle | Rhiannon Bates Wei Huang Lei Cao Adipose Tissue: An Emerging Target for Adeno-associated Viral Vectors Molecular Therapy: Methods & Clinical Development AAV adipose gene therapy tissue tropism capsid engineering |
| title | Adipose Tissue: An Emerging Target for Adeno-associated Viral Vectors |
| title_full | Adipose Tissue: An Emerging Target for Adeno-associated Viral Vectors |
| title_fullStr | Adipose Tissue: An Emerging Target for Adeno-associated Viral Vectors |
| title_full_unstemmed | Adipose Tissue: An Emerging Target for Adeno-associated Viral Vectors |
| title_short | Adipose Tissue: An Emerging Target for Adeno-associated Viral Vectors |
| title_sort | adipose tissue an emerging target for adeno associated viral vectors |
| topic | AAV adipose gene therapy tissue tropism capsid engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2329050120301947 |
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