Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes
Drosophila dorsal air sac development depends on Decapentaplegic (Dpp) and Fibroblast growth factor (FGF) proteins produced by the wing imaginal disc and transported by cytonemes to the air sac primordium (ASP). Dpp and FGF signaling in the ASP was dependent on components of the planar cell polarity...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/18979 |
| _version_ | 1818018590790516736 |
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| author | Hai Huang Thomas B Kornberg |
| author_facet | Hai Huang Thomas B Kornberg |
| author_sort | Hai Huang |
| collection | DOAJ |
| description | Drosophila dorsal air sac development depends on Decapentaplegic (Dpp) and Fibroblast growth factor (FGF) proteins produced by the wing imaginal disc and transported by cytonemes to the air sac primordium (ASP). Dpp and FGF signaling in the ASP was dependent on components of the planar cell polarity (PCP) system in the disc, and neither Dpp- nor FGF-receiving cytonemes extended over mutant disc cells that lacked them. ASP cytonemes normally navigate through extracellular matrix (ECM) composed of collagen, laminin, Dally and Dally-like (Dlp) proteins that are stratified in layers over the disc cells. However, ECM over PCP mutant cells had reduced levels of laminin, Dally and Dlp, and whereas Dpp-receiving ASP cytonemes navigated in the Dally layer and required Dally (but not Dlp), FGF-receiving ASP cytonemes navigated in the Dlp layer, requiring Dlp (but not Dally). These findings suggest that cytonemes interact directly and specifically with proteins in the stratified ECM. |
| first_indexed | 2024-04-14T07:41:40Z |
| format | Article |
| id | doaj.art-b7d841a518cc463a9bbbe727352be4f3 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:41:40Z |
| publishDate | 2016-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-b7d841a518cc463a9bbbe727352be4f32022-12-22T02:05:28ZengeLife Sciences Publications LtdeLife2050-084X2016-09-01510.7554/eLife.18979Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemesHai Huang0Thomas B Kornberg1https://orcid.org/0000-0002-6879-7066Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United StatesCardiovascular Research Institute, University of California, San Francisco, San Francisco, United StatesDrosophila dorsal air sac development depends on Decapentaplegic (Dpp) and Fibroblast growth factor (FGF) proteins produced by the wing imaginal disc and transported by cytonemes to the air sac primordium (ASP). Dpp and FGF signaling in the ASP was dependent on components of the planar cell polarity (PCP) system in the disc, and neither Dpp- nor FGF-receiving cytonemes extended over mutant disc cells that lacked them. ASP cytonemes normally navigate through extracellular matrix (ECM) composed of collagen, laminin, Dally and Dally-like (Dlp) proteins that are stratified in layers over the disc cells. However, ECM over PCP mutant cells had reduced levels of laminin, Dally and Dlp, and whereas Dpp-receiving ASP cytonemes navigated in the Dally layer and required Dally (but not Dlp), FGF-receiving ASP cytonemes navigated in the Dlp layer, requiring Dlp (but not Dally). These findings suggest that cytonemes interact directly and specifically with proteins in the stratified ECM.https://elifesciences.org/articles/18979cytonemeHeparan sulfate proteoglycanplanar cell polarityextracellular matrix |
| spellingShingle | Hai Huang Thomas B Kornberg Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes eLife cytoneme Heparan sulfate proteoglycan planar cell polarity extracellular matrix |
| title | Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes |
| title_full | Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes |
| title_fullStr | Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes |
| title_full_unstemmed | Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes |
| title_short | Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes |
| title_sort | cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes |
| topic | cytoneme Heparan sulfate proteoglycan planar cell polarity extracellular matrix |
| url | https://elifesciences.org/articles/18979 |
| work_keys_str_mv | AT haihuang cellsmustexpresscomponentsoftheplanarcellpolaritysystemandextracellularmatrixtosupportcytonemes AT thomasbkornberg cellsmustexpresscomponentsoftheplanarcellpolaritysystemandextracellularmatrixtosupportcytonemes |