Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew in Cucumber (<i>Cucumis sativus</i> L.) through a Different Mechanism Than That of Heat Shock-Induced Resistance
Heat shock treatment (HST) and UV-B irradiation can reduce pathogen infection in crops. However, information on the mechanism of UV-B action is limited. Here, we investigated the mechanism of UV-B-induced resistance against powdery mildew in cucumber and compared it to that of heat-shock-induced res...
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| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/12/3011 |
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| author | Dinar Mindrati Fardhani Agung Dian Kharisma Tomoyuki Kobayashi Nur Akbar Arofatullah Makoto Yamada Sayuri Tanabata Yumi Yokoda Ani Widiastuti Tatsuo Sato |
| author_facet | Dinar Mindrati Fardhani Agung Dian Kharisma Tomoyuki Kobayashi Nur Akbar Arofatullah Makoto Yamada Sayuri Tanabata Yumi Yokoda Ani Widiastuti Tatsuo Sato |
| author_sort | Dinar Mindrati Fardhani |
| collection | DOAJ |
| description | Heat shock treatment (HST) and UV-B irradiation can reduce pathogen infection in crops. However, information on the mechanism of UV-B action is limited. Here, we investigated the mechanism of UV-B-induced resistance against powdery mildew in cucumber and compared it to that of heat-shock-induced resistance. We measured the percentage of leaf area showing disease symptoms and examined the expression levels of defense- and heat-shock-related genes across treatment groups. UV-B irradiation (intensity, 5 µW/cm<sup>2</sup>) for 4 h/d followed by pathogen inoculation reduced the appearance of powdery mildew by 21.17% compared with the control group. Unlike HST—which induces systemic resistance—UV-B irradiation induced local resistance in cucumber, as indicated by local changes in gene expression (<i>Chi2</i> and <i>ETR2</i>). UV-B-treated plants inoculated with powdery mildew showed higher expression levels of <i>Chi2</i>, <i>ETR2</i>, and <i>LOX6</i> than plants that were either treated with UV-B or inoculated. UV-B had no major effects on systemic acquired resistance or heat shock transcription factors, which are known to be affected by HST. Combined HST and UV-B had a strong synergistic effect in reducing powdery mildew in cucumber. Our results indicate that UV-B treatment likely operates through a different mechanism than HST in triggering cucumber resistance against powdery mildew infection. |
| first_indexed | 2024-03-09T17:26:14Z |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T17:26:14Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-b7d8d6a8889547b0a368816baa79ffd32023-11-24T12:44:55ZengMDPI AGAgronomy2073-43952022-11-011212301110.3390/agronomy12123011Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew in Cucumber (<i>Cucumis sativus</i> L.) through a Different Mechanism Than That of Heat Shock-Induced ResistanceDinar Mindrati Fardhani0Agung Dian Kharisma1Tomoyuki Kobayashi2Nur Akbar Arofatullah3Makoto Yamada4Sayuri Tanabata5Yumi Yokoda6Ani Widiastuti7Tatsuo Sato8Faculty of Science and Technology, Universitas Aisyiyah Yogyakarta, Yogyakarta 55592, IndonesiaUnited Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu 183-0054, Tokyo, JapanFukushima Prefectural Government, Fukushima 960-8670, Fukushima, JapanFaculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaPanasonic Corporation, Kadoma 571-8686, Osaka, JapanCenter for International Field Agriculture, College of Agriculture, Ibaraki University, Ami, Inashiki 300-0331, Ibaraki, JapanFukushima Agricultural Technology Centre, Koriyama 963-0531, Fukushima, JapanFaculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaCenter for International Field Agriculture, College of Agriculture, Ibaraki University, Ami, Inashiki 300-0331, Ibaraki, JapanHeat shock treatment (HST) and UV-B irradiation can reduce pathogen infection in crops. However, information on the mechanism of UV-B action is limited. Here, we investigated the mechanism of UV-B-induced resistance against powdery mildew in cucumber and compared it to that of heat-shock-induced resistance. We measured the percentage of leaf area showing disease symptoms and examined the expression levels of defense- and heat-shock-related genes across treatment groups. UV-B irradiation (intensity, 5 µW/cm<sup>2</sup>) for 4 h/d followed by pathogen inoculation reduced the appearance of powdery mildew by 21.17% compared with the control group. Unlike HST—which induces systemic resistance—UV-B irradiation induced local resistance in cucumber, as indicated by local changes in gene expression (<i>Chi2</i> and <i>ETR2</i>). UV-B-treated plants inoculated with powdery mildew showed higher expression levels of <i>Chi2</i>, <i>ETR2</i>, and <i>LOX6</i> than plants that were either treated with UV-B or inoculated. UV-B had no major effects on systemic acquired resistance or heat shock transcription factors, which are known to be affected by HST. Combined HST and UV-B had a strong synergistic effect in reducing powdery mildew in cucumber. Our results indicate that UV-B treatment likely operates through a different mechanism than HST in triggering cucumber resistance against powdery mildew infection.https://www.mdpi.com/2073-4395/12/12/3011defense-related geneslocal induced resistanceplant defense responseultraviolet-B |
| spellingShingle | Dinar Mindrati Fardhani Agung Dian Kharisma Tomoyuki Kobayashi Nur Akbar Arofatullah Makoto Yamada Sayuri Tanabata Yumi Yokoda Ani Widiastuti Tatsuo Sato Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew in Cucumber (<i>Cucumis sativus</i> L.) through a Different Mechanism Than That of Heat Shock-Induced Resistance Agronomy defense-related genes local induced resistance plant defense response ultraviolet-B |
| title | Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew in Cucumber (<i>Cucumis sativus</i> L.) through a Different Mechanism Than That of Heat Shock-Induced Resistance |
| title_full | Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew in Cucumber (<i>Cucumis sativus</i> L.) through a Different Mechanism Than That of Heat Shock-Induced Resistance |
| title_fullStr | Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew in Cucumber (<i>Cucumis sativus</i> L.) through a Different Mechanism Than That of Heat Shock-Induced Resistance |
| title_full_unstemmed | Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew in Cucumber (<i>Cucumis sativus</i> L.) through a Different Mechanism Than That of Heat Shock-Induced Resistance |
| title_short | Ultraviolet-B Irradiation Induces Resistance against Powdery Mildew in Cucumber (<i>Cucumis sativus</i> L.) through a Different Mechanism Than That of Heat Shock-Induced Resistance |
| title_sort | ultraviolet b irradiation induces resistance against powdery mildew in cucumber i cucumis sativus i l through a different mechanism than that of heat shock induced resistance |
| topic | defense-related genes local induced resistance plant defense response ultraviolet-B |
| url | https://www.mdpi.com/2073-4395/12/12/3011 |
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