The mechanism of oleic acid inhibiting platelet activation stimulated by collagen
Abstract Background Abnormal platelet activation is a key factor in the occurrence and development of thrombotic diseases. However, the physiological mechanisms that underlie platelet homeostasis remain unclear. Oleic acid, one of the most abundant lipids in the human diet, has potential antithrombo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | Cell Communication and Signaling |
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| Online Access: | https://doi.org/10.1186/s12964-023-01276-0 |
| _version_ | 1797557741691076608 |
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| author | Xianghui Zhou Xin Zhou Ruirui Zhu Zhangyin Ming Zhipeng Cheng Yu Hu |
| author_facet | Xianghui Zhou Xin Zhou Ruirui Zhu Zhangyin Ming Zhipeng Cheng Yu Hu |
| author_sort | Xianghui Zhou |
| collection | DOAJ |
| description | Abstract Background Abnormal platelet activation is a key factor in the occurrence and development of thrombotic diseases. However, the physiological mechanisms that underlie platelet homeostasis remain unclear. Oleic acid, one of the most abundant lipids in the human diet, has potential antithrombotic effects. This study aimed to investigate the effects of oleic acid on platelet activation and thrombosis. Methods Platelet aggregation, ATP release, and fibrinogen spread were evaluated to determine the role of oleic acid in platelet activation. A ferric chloride-induced carotid injury model was used to establish the effect of oleic acid on thrombus formation in vivo. Western blotting analysis and transfection experiments were performed to determine the mechanisms involved in this process. Results Oleic acid inhibited platelet aggregation, granule release, and calcium mobilization. Furthermore, it inhibited the spread of platelets on fibrinogen. We also found that oleic acid delayed arterial thrombosis in mice, as demonstrated in a murine model of ferric chloride-induced carotid artery thrombosis. The molecular mechanism of its inhibition of platelet activity may be through the Syk-PLCγ2 and CaMKKβ/AMPKα/VASP pathways. In addition, we demonstrated that the phosphorylation of AMPK at Ser496 was an important mechanism of platelet activation. Conclusions Our study showed that oleic acid inhibits platelet activation and reduces thrombogenesis by inhibiting the phosphorylation of multiple signaling molecules, offering new insights into the research and development of antiplatelet drugs. Video Abstract |
| first_indexed | 2024-03-10T17:20:43Z |
| format | Article |
| id | doaj.art-b7d9df68005a4c848fe3b9da08363ccc |
| institution | Directory Open Access Journal |
| issn | 1478-811X |
| language | English |
| last_indexed | 2024-03-10T17:20:43Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj.art-b7d9df68005a4c848fe3b9da08363ccc2023-11-20T10:22:04ZengBMCCell Communication and Signaling1478-811X2023-10-0121111410.1186/s12964-023-01276-0The mechanism of oleic acid inhibiting platelet activation stimulated by collagenXianghui Zhou0Xin Zhou1Ruirui Zhu2Zhangyin Ming3Zhipeng Cheng4Yu Hu5Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Pharmacology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Background Abnormal platelet activation is a key factor in the occurrence and development of thrombotic diseases. However, the physiological mechanisms that underlie platelet homeostasis remain unclear. Oleic acid, one of the most abundant lipids in the human diet, has potential antithrombotic effects. This study aimed to investigate the effects of oleic acid on platelet activation and thrombosis. Methods Platelet aggregation, ATP release, and fibrinogen spread were evaluated to determine the role of oleic acid in platelet activation. A ferric chloride-induced carotid injury model was used to establish the effect of oleic acid on thrombus formation in vivo. Western blotting analysis and transfection experiments were performed to determine the mechanisms involved in this process. Results Oleic acid inhibited platelet aggregation, granule release, and calcium mobilization. Furthermore, it inhibited the spread of platelets on fibrinogen. We also found that oleic acid delayed arterial thrombosis in mice, as demonstrated in a murine model of ferric chloride-induced carotid artery thrombosis. The molecular mechanism of its inhibition of platelet activity may be through the Syk-PLCγ2 and CaMKKβ/AMPKα/VASP pathways. In addition, we demonstrated that the phosphorylation of AMPK at Ser496 was an important mechanism of platelet activation. Conclusions Our study showed that oleic acid inhibits platelet activation and reduces thrombogenesis by inhibiting the phosphorylation of multiple signaling molecules, offering new insights into the research and development of antiplatelet drugs. Video Abstracthttps://doi.org/10.1186/s12964-023-01276-0Arterial thrombosisOleic acidPlatelet aggregation |
| spellingShingle | Xianghui Zhou Xin Zhou Ruirui Zhu Zhangyin Ming Zhipeng Cheng Yu Hu The mechanism of oleic acid inhibiting platelet activation stimulated by collagen Cell Communication and Signaling Arterial thrombosis Oleic acid Platelet aggregation |
| title | The mechanism of oleic acid inhibiting platelet activation stimulated by collagen |
| title_full | The mechanism of oleic acid inhibiting platelet activation stimulated by collagen |
| title_fullStr | The mechanism of oleic acid inhibiting platelet activation stimulated by collagen |
| title_full_unstemmed | The mechanism of oleic acid inhibiting platelet activation stimulated by collagen |
| title_short | The mechanism of oleic acid inhibiting platelet activation stimulated by collagen |
| title_sort | mechanism of oleic acid inhibiting platelet activation stimulated by collagen |
| topic | Arterial thrombosis Oleic acid Platelet aggregation |
| url | https://doi.org/10.1186/s12964-023-01276-0 |
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