GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level
Calcium-Dependent Protein Kinases (CDPKs) involved in regulating downstream components of calcium signaling pathways play a role in tolerance to abiotic stresses and seed development in plants. However, functions of only a few cotton CDPKs have been clarified at present. In this study, 80 conserved...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1072584/full |
| _version_ | 1828098025169354752 |
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| author | Mengyuan Yan Xiaotian Yu Gen Zhou Dongli Sun Yu Hu Chenjue Huang Qintao Zheng Nan Sun Jiayan Wu Zhaobin Fu Libei Li Zhen Feng Shuxun Yu Shuxun Yu |
| author_facet | Mengyuan Yan Xiaotian Yu Gen Zhou Dongli Sun Yu Hu Chenjue Huang Qintao Zheng Nan Sun Jiayan Wu Zhaobin Fu Libei Li Zhen Feng Shuxun Yu Shuxun Yu |
| author_sort | Mengyuan Yan |
| collection | DOAJ |
| description | Calcium-Dependent Protein Kinases (CDPKs) involved in regulating downstream components of calcium signaling pathways play a role in tolerance to abiotic stresses and seed development in plants. However, functions of only a few cotton CDPKs have been clarified at present. In this study, 80 conserved CDPKs in Gossypium hirsutum L. were identified and characterized, which was divided into four subgroups. Among them, the transcript level of GhCDPK60 was significantly upregulated under drought and several hormone treatments. And we found that the expression levels of several stress-inducible genes down-regulated in GhCDPK60-silence cotton and up-regulated in GhCDPK60-overexpressing Arabidopsis. In addition, physiological analyses demonstrated that GhCDPK60 improved drought stress tolerance by improving the osmotic adjustment ability and reducing the accumulation of reactive oxygen species (ROS) in plants. These findings broaden our understanding of the biological roles of GhCDPK60 and mechanisms underlying drought stress tolerance in cotton. |
| first_indexed | 2024-04-11T07:55:06Z |
| format | Article |
| id | doaj.art-b7da51602f0c4d68a9eb1afda9a6a66c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T07:55:06Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b7da51602f0c4d68a9eb1afda9a6a66c2022-12-22T04:35:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-12-011310.3389/fpls.2022.10725841072584GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS levelMengyuan Yan0Xiaotian Yu1Gen Zhou2Dongli Sun3Yu Hu4Chenjue Huang5Qintao Zheng6Nan Sun7Jiayan Wu8Zhaobin Fu9Libei Li10Zhen Feng11Shuxun Yu12Shuxun Yu13College of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaCollege of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan, ChinaCalcium-Dependent Protein Kinases (CDPKs) involved in regulating downstream components of calcium signaling pathways play a role in tolerance to abiotic stresses and seed development in plants. However, functions of only a few cotton CDPKs have been clarified at present. In this study, 80 conserved CDPKs in Gossypium hirsutum L. were identified and characterized, which was divided into four subgroups. Among them, the transcript level of GhCDPK60 was significantly upregulated under drought and several hormone treatments. And we found that the expression levels of several stress-inducible genes down-regulated in GhCDPK60-silence cotton and up-regulated in GhCDPK60-overexpressing Arabidopsis. In addition, physiological analyses demonstrated that GhCDPK60 improved drought stress tolerance by improving the osmotic adjustment ability and reducing the accumulation of reactive oxygen species (ROS) in plants. These findings broaden our understanding of the biological roles of GhCDPK60 and mechanisms underlying drought stress tolerance in cotton.https://www.frontiersin.org/articles/10.3389/fpls.2022.1072584/fullGossypium hirsutum L.GhCDPK60drought stressreactive oxygen species (ROS)osmotic adjustment (OA) |
| spellingShingle | Mengyuan Yan Xiaotian Yu Gen Zhou Dongli Sun Yu Hu Chenjue Huang Qintao Zheng Nan Sun Jiayan Wu Zhaobin Fu Libei Li Zhen Feng Shuxun Yu Shuxun Yu GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level Frontiers in Plant Science Gossypium hirsutum L. GhCDPK60 drought stress reactive oxygen species (ROS) osmotic adjustment (OA) |
| title | GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level |
| title_full | GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level |
| title_fullStr | GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level |
| title_full_unstemmed | GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level |
| title_short | GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level |
| title_sort | ghcdpk60 positively regulates drought stress tolerance in both transgenic arabidopsis and cotton by regulating proline content and ros level |
| topic | Gossypium hirsutum L. GhCDPK60 drought stress reactive oxygen species (ROS) osmotic adjustment (OA) |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1072584/full |
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