Immunomodulating Activity of <i>Pleurotus eryngii</i> Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota
Recent studies have revealed the crucial role of several edible mushrooms and fungal compounds, mainly polysaccharides, in human health and disease. The investigation of the immunomodulating effects of mushroom polysaccharides, especially β-glucans, and the link between their anticancer and immunomo...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/8/4/329 |
| _version_ | 1797434516120272896 |
|---|---|
| author | Marigoula Vlassopoulou Nikolaos Paschalidis Alexandros L. Savvides Georgia Saxami Evdokia K. Mitsou Evangelia N. Kerezoudi Georgios Koutrotsios Georgios I. Zervakis Panagiotis Georgiadis Adamantini Kyriacou Vasiliki Pletsa |
| author_facet | Marigoula Vlassopoulou Nikolaos Paschalidis Alexandros L. Savvides Georgia Saxami Evdokia K. Mitsou Evangelia N. Kerezoudi Georgios Koutrotsios Georgios I. Zervakis Panagiotis Georgiadis Adamantini Kyriacou Vasiliki Pletsa |
| author_sort | Marigoula Vlassopoulou |
| collection | DOAJ |
| description | Recent studies have revealed the crucial role of several edible mushrooms and fungal compounds, mainly polysaccharides, in human health and disease. The investigation of the immunomodulating effects of mushroom polysaccharides, especially β-glucans, and the link between their anticancer and immunomodulatory properties with their possible prebiotic activity on gut micro-organisms has been the subject of intense research over the last decade. We investigated the immunomodulating effects of <i>Pleurotus eryngii</i> mushrooms, selected due to their high β-glucan content, strong lactogenic effect, and potent geno-protective properties, following in vitro fermentation by fecal inocula from healthy elderly volunteers (>60 years old). The immunomodulating properties of the fermentation supernatants (FSs) were initially investigated in U937-derived human macrophages. Gene expression as well as pro- (TNF-α, IL-1β) and anti-inflammatory cytokines (IL-10, IL-1Rα) were assessed and correlated with the fermentation process. The presence of <i>P. eryngii</i> in the fermentation process led to modifications in immune response, as indicated by the altered gene expression and levels of the cytokines examined, a finding consistent for all volunteers. The FSs immunomodulating effect on the volunteers’ peripheral blood mononuclear cells (PBMCs) was verified through the use of cytometry by time of flight (CyTOF) analysis. |
| first_indexed | 2024-03-09T10:33:24Z |
| format | Article |
| id | doaj.art-b7dadf3bef1f43309308cf36cd59f01e |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T10:33:24Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-b7dadf3bef1f43309308cf36cd59f01e2023-12-01T21:08:13ZengMDPI AGJournal of Fungi2309-608X2022-03-018432910.3390/jof8040329Immunomodulating Activity of <i>Pleurotus eryngii</i> Mushrooms Following Their In Vitro Fermentation by Human Fecal MicrobiotaMarigoula Vlassopoulou0Nikolaos Paschalidis1Alexandros L. Savvides2Georgia Saxami3Evdokia K. Mitsou4Evangelia N. Kerezoudi5Georgios Koutrotsios6Georgios I. Zervakis7Panagiotis Georgiadis8Adamantini Kyriacou9Vasiliki Pletsa10Institute of Chemical Biology, National Hellenic Research Foundation, 11635 Athens, GreeceCyTOF Laboratory, Biomedical Research Foundation of the Academy of Athens (BRFAA), 11527 Athens, GreeceMicrobiology Group, Department of Botany, Faculty of Biology, National and Kapodistrian University of Athens, Panepistimioupolis, Zografou, 15781 Athens, GreeceDepartment of Nutrition and Dietetics, Harokopio University, 17671 Athens, GreeceDepartment of Nutrition and Dietetics, Harokopio University, 17671 Athens, GreeceDepartment of Nutrition and Dietetics, Harokopio University, 17671 Athens, GreeceLaboratory of General and Agricultural Microbiology, Department of Crop Science, Agricultural University of Athens, 11855 Athens, GreeceLaboratory of General and Agricultural Microbiology, Department of Crop Science, Agricultural University of Athens, 11855 Athens, GreeceInstitute of Chemical Biology, National Hellenic Research Foundation, 11635 Athens, GreeceDepartment of Nutrition and Dietetics, Harokopio University, 17671 Athens, GreeceInstitute of Chemical Biology, National Hellenic Research Foundation, 11635 Athens, GreeceRecent studies have revealed the crucial role of several edible mushrooms and fungal compounds, mainly polysaccharides, in human health and disease. The investigation of the immunomodulating effects of mushroom polysaccharides, especially β-glucans, and the link between their anticancer and immunomodulatory properties with their possible prebiotic activity on gut micro-organisms has been the subject of intense research over the last decade. We investigated the immunomodulating effects of <i>Pleurotus eryngii</i> mushrooms, selected due to their high β-glucan content, strong lactogenic effect, and potent geno-protective properties, following in vitro fermentation by fecal inocula from healthy elderly volunteers (>60 years old). The immunomodulating properties of the fermentation supernatants (FSs) were initially investigated in U937-derived human macrophages. Gene expression as well as pro- (TNF-α, IL-1β) and anti-inflammatory cytokines (IL-10, IL-1Rα) were assessed and correlated with the fermentation process. The presence of <i>P. eryngii</i> in the fermentation process led to modifications in immune response, as indicated by the altered gene expression and levels of the cytokines examined, a finding consistent for all volunteers. The FSs immunomodulating effect on the volunteers’ peripheral blood mononuclear cells (PBMCs) was verified through the use of cytometry by time of flight (CyTOF) analysis.https://www.mdpi.com/2309-608X/8/4/329edible mushroomsmacrophagescytokinesPBMCs |
| spellingShingle | Marigoula Vlassopoulou Nikolaos Paschalidis Alexandros L. Savvides Georgia Saxami Evdokia K. Mitsou Evangelia N. Kerezoudi Georgios Koutrotsios Georgios I. Zervakis Panagiotis Georgiadis Adamantini Kyriacou Vasiliki Pletsa Immunomodulating Activity of <i>Pleurotus eryngii</i> Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota Journal of Fungi edible mushrooms macrophages cytokines PBMCs |
| title | Immunomodulating Activity of <i>Pleurotus eryngii</i> Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota |
| title_full | Immunomodulating Activity of <i>Pleurotus eryngii</i> Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota |
| title_fullStr | Immunomodulating Activity of <i>Pleurotus eryngii</i> Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota |
| title_full_unstemmed | Immunomodulating Activity of <i>Pleurotus eryngii</i> Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota |
| title_short | Immunomodulating Activity of <i>Pleurotus eryngii</i> Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota |
| title_sort | immunomodulating activity of i pleurotus eryngii i mushrooms following their in vitro fermentation by human fecal microbiota |
| topic | edible mushrooms macrophages cytokines PBMCs |
| url | https://www.mdpi.com/2309-608X/8/4/329 |
| work_keys_str_mv | AT marigoulavlassopoulou immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota AT nikolaospaschalidis immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota AT alexandroslsavvides immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota AT georgiasaxami immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota AT evdokiakmitsou immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota AT evangeliankerezoudi immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota AT georgioskoutrotsios immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota AT georgiosizervakis immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota AT panagiotisgeorgiadis immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota AT adamantinikyriacou immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota AT vasilikipletsa immunomodulatingactivityofipleurotuseryngiiimushroomsfollowingtheirinvitrofermentationbyhumanfecalmicrobiota |