DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes
Objective: To investigate the phytochemical constituents, antioxidant and anthelmintic activities of the crude methanol extract of Cissus populnea (C. populnea) rhizomes. Methods: Phytochemical screening was performed using standard protocols, and column chromatography of silica gel was used for the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Asian Pacific Journal of Tropical Medicine |
| Subjects: | |
| Online Access: | http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=4;spage=280;epage=284;aulast=Nyemb |
| _version_ | 1818451485698031616 |
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| author | Jean Noël Nyemb Roland Ndoubalem Emmanuel Talla Alembert Tiabou Tchinda Dieudonné Ndjonka Celine Henoumont Sophie Laurent Joseph Tanyi Mbafor |
| author_facet | Jean Noël Nyemb Roland Ndoubalem Emmanuel Talla Alembert Tiabou Tchinda Dieudonné Ndjonka Celine Henoumont Sophie Laurent Joseph Tanyi Mbafor |
| author_sort | Jean Noël Nyemb |
| collection | DOAJ |
| description | Objective: To investigate the phytochemical constituents, antioxidant and anthelmintic activities of the crude methanol extract of Cissus populnea (C. populnea) rhizomes. Methods: Phytochemical screening was performed using standard protocols, and column chromatography of silica gel was used for the compounds isolation. DPPH antiradical scavenging assay was performed in order to evaluate the antioxidant activity. Total phenolic content was evaluated using the Folin–Ciocalteu assay. The anthelmintic activity was screened on the bovine adult male forms of parasitic nematode Onchocerca ochengi, by the in vitro evaluation of the inhibition of adult worm motility and mortality. Worms were incubated in the presence of different concentrations of the plant extract and effects on survival were monitored after 24 and 48 h. Results: The preliminary phytochemical screening revealed the presence of phenolic compounds, saponins, steroids, tannins, and terpenoids. Bergenin and a mixture of phytosterol, β -sitosterol and stigmasterol were isolated from this extract and were identified by nuclear magnetic resonance, mass spectrometry and by comparison with published data. The crude methanol extract of C. populnea rhizomes showed a strong DPPH antiradical activity with a good amount of total phenolic content ((20.69±2.13) g gallic acid equivalent/100 g of extract) and significant anthelmintic activity comparable to the standard drug ivermectin. Bergenin was found to be inactive even after 72 h of incubation. Conclusions: This study constitutes the first report on the anthelmintic activity of this plant and supports the traditional use of C. populnea as a natural antioxidant and anthelmintic. |
| first_indexed | 2024-12-14T21:07:57Z |
| format | Article |
| id | doaj.art-b7db1ce7ca11459b9e95b4c756fe6323 |
| institution | Directory Open Access Journal |
| issn | 2352-4146 |
| language | English |
| last_indexed | 2024-12-14T21:07:57Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Medicine |
| spelling | doaj.art-b7db1ce7ca11459b9e95b4c756fe63232022-12-21T22:47:23ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462018-01-0111428028410.4103/1995-7645.231468DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomesJean Noël NyembRoland NdoubalemEmmanuel TallaAlembert Tiabou TchindaDieudonné NdjonkaCeline HenoumontSophie LaurentJoseph Tanyi MbaforObjective: To investigate the phytochemical constituents, antioxidant and anthelmintic activities of the crude methanol extract of Cissus populnea (C. populnea) rhizomes. Methods: Phytochemical screening was performed using standard protocols, and column chromatography of silica gel was used for the compounds isolation. DPPH antiradical scavenging assay was performed in order to evaluate the antioxidant activity. Total phenolic content was evaluated using the Folin–Ciocalteu assay. The anthelmintic activity was screened on the bovine adult male forms of parasitic nematode Onchocerca ochengi, by the in vitro evaluation of the inhibition of adult worm motility and mortality. Worms were incubated in the presence of different concentrations of the plant extract and effects on survival were monitored after 24 and 48 h. Results: The preliminary phytochemical screening revealed the presence of phenolic compounds, saponins, steroids, tannins, and terpenoids. Bergenin and a mixture of phytosterol, β -sitosterol and stigmasterol were isolated from this extract and were identified by nuclear magnetic resonance, mass spectrometry and by comparison with published data. The crude methanol extract of C. populnea rhizomes showed a strong DPPH antiradical activity with a good amount of total phenolic content ((20.69±2.13) g gallic acid equivalent/100 g of extract) and significant anthelmintic activity comparable to the standard drug ivermectin. Bergenin was found to be inactive even after 72 h of incubation. Conclusions: This study constitutes the first report on the anthelmintic activity of this plant and supports the traditional use of C. populnea as a natural antioxidant and anthelmintic.http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=4;spage=280;epage=284;aulast=Nyembcissus populneaonchocerca ochengiantioxidantphenolic compoundsphytochemical constituentbergenin |
| spellingShingle | Jean Noël Nyemb Roland Ndoubalem Emmanuel Talla Alembert Tiabou Tchinda Dieudonné Ndjonka Celine Henoumont Sophie Laurent Joseph Tanyi Mbafor DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes Asian Pacific Journal of Tropical Medicine cissus populnea onchocerca ochengi antioxidant phenolic compounds phytochemical constituent bergenin |
| title | DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
| title_full | DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
| title_fullStr | DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
| title_full_unstemmed | DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
| title_short | DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
| title_sort | dpph antiradical scavenging anthelmintic and phytochemical studies of cissus poulnea rhizomes |
| topic | cissus populnea onchocerca ochengi antioxidant phenolic compounds phytochemical constituent bergenin |
| url | http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=4;spage=280;epage=284;aulast=Nyemb |
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