Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1
Abstract Background Mounting evidence supports that long noncoding RNAs (lncRNAs) have critical roles during cancer initiation and progression. In this study, we report that the plasmacytoma variant translocation 1 (PVT1) lncRNA is involved in breast cancer progression. Methods qRT-PCR and western b...
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | Breast Cancer Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13058-021-01491-y |
| _version_ | 1797736429022871552 |
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| author | Shuiyi Liu Weiqun Chen Hui Hu Tianzhu Zhang Tangwei Wu Xiaoyi Li Yong Li Qinzhi Kong Hongda Lu Zhongxin Lu |
| author_facet | Shuiyi Liu Weiqun Chen Hui Hu Tianzhu Zhang Tangwei Wu Xiaoyi Li Yong Li Qinzhi Kong Hongda Lu Zhongxin Lu |
| author_sort | Shuiyi Liu |
| collection | DOAJ |
| description | Abstract Background Mounting evidence supports that long noncoding RNAs (lncRNAs) have critical roles during cancer initiation and progression. In this study, we report that the plasmacytoma variant translocation 1 (PVT1) lncRNA is involved in breast cancer progression. Methods qRT-PCR and western blot were performed to detect the gene and protein expression. Colony formation would healing and transwell assays were used to detect cell function. Dual-luciferase reporter assay and RNA pull-down experiments were used to examine the mechanisms interaction between molecules. Orthotopic mouse models were established to evaluate the influence of PVT1 on tumor growth and metastasis in vivo. Results PVT1 is significant upregulated in breast cancer patients’ plasma and cell lines. PVT1 promotes breast cancer cell proliferation and metastasis both in vitro and in vivo. Mechanistically, PVT1 upregulates FOXQ1 via miR-128-3p and promotes epithelial–mesenchymal transition. In addition, PVT1 binds to the UPF1 protein, thereby inducing epithelial–mesenchymal transition, proliferation and metastasis in breast cancer cells. Conclusion PVT1 may act as an oncogene in breast cancer through binding miR-128-3p and UPF1 and represents a potential target for BC therapeutic development. |
| first_indexed | 2024-03-12T13:12:41Z |
| format | Article |
| id | doaj.art-b7def1aae7bc4dfca2cfe38841b5621c |
| institution | Directory Open Access Journal |
| issn | 1465-542X |
| language | English |
| last_indexed | 2024-03-12T13:12:41Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Breast Cancer Research |
| spelling | doaj.art-b7def1aae7bc4dfca2cfe38841b5621c2023-08-27T11:32:31ZengBMCBreast Cancer Research1465-542X2021-12-0123111310.1186/s13058-021-01491-yLong noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1Shuiyi Liu0Weiqun Chen1Hui Hu2Tianzhu Zhang3Tangwei Wu4Xiaoyi Li5Yong Li6Qinzhi Kong7Hongda Lu8Zhongxin Lu9Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Medicine, Dan L Duncan Comprehensive Cancer Center, Baylor College of MedicineCancer Research Institute of Wuhan, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and TechnologyCancer Research Institute of Wuhan, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Background Mounting evidence supports that long noncoding RNAs (lncRNAs) have critical roles during cancer initiation and progression. In this study, we report that the plasmacytoma variant translocation 1 (PVT1) lncRNA is involved in breast cancer progression. Methods qRT-PCR and western blot were performed to detect the gene and protein expression. Colony formation would healing and transwell assays were used to detect cell function. Dual-luciferase reporter assay and RNA pull-down experiments were used to examine the mechanisms interaction between molecules. Orthotopic mouse models were established to evaluate the influence of PVT1 on tumor growth and metastasis in vivo. Results PVT1 is significant upregulated in breast cancer patients’ plasma and cell lines. PVT1 promotes breast cancer cell proliferation and metastasis both in vitro and in vivo. Mechanistically, PVT1 upregulates FOXQ1 via miR-128-3p and promotes epithelial–mesenchymal transition. In addition, PVT1 binds to the UPF1 protein, thereby inducing epithelial–mesenchymal transition, proliferation and metastasis in breast cancer cells. Conclusion PVT1 may act as an oncogene in breast cancer through binding miR-128-3p and UPF1 and represents a potential target for BC therapeutic development.https://doi.org/10.1186/s13058-021-01491-yPlasmacytoma variant translocation 1Breast cancerEpithelial–mesenchymal transitionmiR-128-3pUp-frameshift protein 1 |
| spellingShingle | Shuiyi Liu Weiqun Chen Hui Hu Tianzhu Zhang Tangwei Wu Xiaoyi Li Yong Li Qinzhi Kong Hongda Lu Zhongxin Lu Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1 Breast Cancer Research Plasmacytoma variant translocation 1 Breast cancer Epithelial–mesenchymal transition miR-128-3p Up-frameshift protein 1 |
| title | Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1 |
| title_full | Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1 |
| title_fullStr | Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1 |
| title_full_unstemmed | Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1 |
| title_short | Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1 |
| title_sort | long noncoding rna pvt1 promotes breast cancer proliferation and metastasis by binding mir 128 3p and upf1 |
| topic | Plasmacytoma variant translocation 1 Breast cancer Epithelial–mesenchymal transition miR-128-3p Up-frameshift protein 1 |
| url | https://doi.org/10.1186/s13058-021-01491-y |
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