Association of the IRF6 rs2235371 and rs861019 Polymorphisms with Non-Syndromic Cleft Lip with or without Cleft Palate in the Yakut Population
Background: Non-syndromic cleft lip with or without cleft palate (NSCL/P) is one of the most common birth defects. NSCL/P can be broadly divided into cleft lip only (CLO), cleft palate only (CPO), and cleft lip with cleft palate (CLP) based on clinical presentation. The aim of this study was to inve...
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| Format: | Article |
| Language: | English |
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International Medical Research and Development Corporation
2021-12-01
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| Series: | International Journal of Biomedicine |
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| Online Access: | http://ijbm.org/articles/i44/ijbm_11(4)_oa28.pdf |
| _version_ | 1819102266540425216 |
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| author | Nadezhda I. Pavlova Aleksandra T. Diakonova Vladislav A. Alekseev Lyubov S. Mironova Vladimir V. Dodokhov Khariton A. Kurtanov Innokenty D. Ushnitsky |
| author_facet | Nadezhda I. Pavlova Aleksandra T. Diakonova Vladislav A. Alekseev Lyubov S. Mironova Vladimir V. Dodokhov Khariton A. Kurtanov Innokenty D. Ushnitsky |
| author_sort | Nadezhda I. Pavlova |
| collection | DOAJ |
| description | Background: Non-syndromic cleft lip with or without cleft palate (NSCL/P) is one of the most common birth defects. NSCL/P can be broadly divided into cleft lip only (CLO), cleft palate only (CPO), and cleft lip with cleft palate (CLP) based on clinical presentation. The aim of this study was to investigate the relationship between the IRF6 gene polymorphisms and non-syndromic cleft lip with or without cleft palate (NSCL/P) in the Yakut population.
Methods and Results: In 23 OFC patients and 58 unrelated control subjects from the Yakut population, we tested two SNPs (rs2235371 and rs861019) with a minor allele frequency of more than 5% in the candidate gene IRF6. We found that the SNP marker rs861019 showed significant differences in allele frequencies (OR=2.07, 95%CI: 1.01–4.23, P=0.04) between the NSCL/P patients and the comparison group. Analysis of allele frequencies for rs861019 SNP in subgroups showed that there was a difference in the frequency between CLP and control (OR=5, 95% CI: 1.61-15.53, P=0.11); however, this result was not significant.
Genotype analysis showed significant differences in patients from the CLP subgroup in comparison with controls for homozygous (AA compared with GG) (OR=9.00, 95% CI: 1.03–78.58, P=0.03), heterozygous (GA compared with GG) (OR=5.50, 95% CI: 1.05-28.75, P=0.04,), recessive (GG compared with GA + AA) (OR=6.67, 95% CI: 1.61-27.58; RR=4.78, 95% CI: 1.42–16.10, P=0.008,) and co-dominant (GG compared with GA, compared with AA) (P=0.02) inheritance models.
Diplotype analysis showed that the NSCL/P group was more likely to have the [CC]-[GG] diplotype than the comparison group. This diplotype carries the risk GG genotype (rs861019) (30.4%) and does not carry the risk T allele(rs2235371). In the CLP subgroup, two diplotypes ([CT]-[GG] and [CC]-[GG]) were found more often than in the comparison group. Both diplotypes carry the risk GG genotype(rs861019; 33.3%). In the CPO subgroup, the [CT]-[GG] diplotype was more common. In the CLO subgroup, only two diplotypes ([CC]-[GA] and [CC]-[GG]) were found, both of which were more common than in the comparison group (75% and 25%).. It is likely that these results for the CLO and CPO subgroups were influenced by the small size of both samples. Unlike the NSCL/P and CLP groups, in these samples, diplotypes with the homozygous genotype GG (rs861019) without the homozygous genotype TT (rs2235371) were more common. Diplotypes with a homozygous genotype of the TT risk allele were not found in the studied groups except for the comparison group, where the [TT]-[AA] diplotype was represented by a low frequency (0.17%).
Conclusion: The present study provides strong statistical support (for the first time to our knowledge) that genetic variants of the IRF6 rs861019 SNP are associated with NSCL/P in Yakuts. |
| first_indexed | 2024-12-22T01:31:50Z |
| format | Article |
| id | doaj.art-b7e43c3a241e4d7eab6a130116963db8 |
| institution | Directory Open Access Journal |
| issn | 2158-0510 2158-0529 |
| language | English |
| last_indexed | 2024-12-22T01:31:50Z |
| publishDate | 2021-12-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj.art-b7e43c3a241e4d7eab6a130116963db82022-12-21T18:43:29ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292021-12-0111457057510.21103/Article11(4)_OA28Association of the IRF6 rs2235371 and rs861019 Polymorphisms with Non-Syndromic Cleft Lip with or without Cleft Palate in the Yakut PopulationNadezhda I. Pavlova0Aleksandra T. Diakonova1Vladislav A. Alekseev2Lyubov S. Mironova3Vladimir V. Dodokhov4Khariton A. Kurtanov5Innokenty D. Ushnitsky6Yakut Science Center of Complex Medical Problems, Yakutsk, the Republic of Sakha (Yakutia), RussiaYakut Science Center of Complex Medical Problems, Yakutsk, the Republic of Sakha (Yakutia), RussiaYakut Science Center of Complex Medical Problems, Yakutsk, the Republic of Sakha (Yakutia), RussiaThe North-Eastern Federal University named after M.K. Ammosov; Republic Hospital No. 1 – National Center of Medicine; Yakutsk, the Republic of Sakha (Yakutia), RussiaArctic State Agrotechnological University; Yakutsk, the Republic of Sakha (Yakutia), RussiaInstitute for Biological Problems of Cryolithozone of SB of RAS; Yakutsk, the Republic of Sakha (Yakutia), RussiaThe North-Eastern Federal University named after M.K. Ammosov, Yakutsk, the Republic of Sakha (Yakutia), RussiaBackground: Non-syndromic cleft lip with or without cleft palate (NSCL/P) is one of the most common birth defects. NSCL/P can be broadly divided into cleft lip only (CLO), cleft palate only (CPO), and cleft lip with cleft palate (CLP) based on clinical presentation. The aim of this study was to investigate the relationship between the IRF6 gene polymorphisms and non-syndromic cleft lip with or without cleft palate (NSCL/P) in the Yakut population. Methods and Results: In 23 OFC patients and 58 unrelated control subjects from the Yakut population, we tested two SNPs (rs2235371 and rs861019) with a minor allele frequency of more than 5% in the candidate gene IRF6. We found that the SNP marker rs861019 showed significant differences in allele frequencies (OR=2.07, 95%CI: 1.01–4.23, P=0.04) between the NSCL/P patients and the comparison group. Analysis of allele frequencies for rs861019 SNP in subgroups showed that there was a difference in the frequency between CLP and control (OR=5, 95% CI: 1.61-15.53, P=0.11); however, this result was not significant. Genotype analysis showed significant differences in patients from the CLP subgroup in comparison with controls for homozygous (AA compared with GG) (OR=9.00, 95% CI: 1.03–78.58, P=0.03), heterozygous (GA compared with GG) (OR=5.50, 95% CI: 1.05-28.75, P=0.04,), recessive (GG compared with GA + AA) (OR=6.67, 95% CI: 1.61-27.58; RR=4.78, 95% CI: 1.42–16.10, P=0.008,) and co-dominant (GG compared with GA, compared with AA) (P=0.02) inheritance models. Diplotype analysis showed that the NSCL/P group was more likely to have the [CC]-[GG] diplotype than the comparison group. This diplotype carries the risk GG genotype (rs861019) (30.4%) and does not carry the risk T allele(rs2235371). In the CLP subgroup, two diplotypes ([CT]-[GG] and [CC]-[GG]) were found more often than in the comparison group. Both diplotypes carry the risk GG genotype(rs861019; 33.3%). In the CPO subgroup, the [CT]-[GG] diplotype was more common. In the CLO subgroup, only two diplotypes ([CC]-[GA] and [CC]-[GG]) were found, both of which were more common than in the comparison group (75% and 25%).. It is likely that these results for the CLO and CPO subgroups were influenced by the small size of both samples. Unlike the NSCL/P and CLP groups, in these samples, diplotypes with the homozygous genotype GG (rs861019) without the homozygous genotype TT (rs2235371) were more common. Diplotypes with a homozygous genotype of the TT risk allele were not found in the studied groups except for the comparison group, where the [TT]-[AA] diplotype was represented by a low frequency (0.17%). Conclusion: The present study provides strong statistical support (for the first time to our knowledge) that genetic variants of the IRF6 rs861019 SNP are associated with NSCL/P in Yakuts.http://ijbm.org/articles/i44/ijbm_11(4)_oa28.pdforofacial cleftirf6 geneyakutshaplotypediplotypers2235371rs861019 |
| spellingShingle | Nadezhda I. Pavlova Aleksandra T. Diakonova Vladislav A. Alekseev Lyubov S. Mironova Vladimir V. Dodokhov Khariton A. Kurtanov Innokenty D. Ushnitsky Association of the IRF6 rs2235371 and rs861019 Polymorphisms with Non-Syndromic Cleft Lip with or without Cleft Palate in the Yakut Population International Journal of Biomedicine orofacial cleft irf6 gene yakuts haplotype diplotype rs2235371 rs861019 |
| title | Association of the IRF6 rs2235371 and rs861019 Polymorphisms with Non-Syndromic Cleft Lip with or without Cleft Palate in the Yakut Population |
| title_full | Association of the IRF6 rs2235371 and rs861019 Polymorphisms with Non-Syndromic Cleft Lip with or without Cleft Palate in the Yakut Population |
| title_fullStr | Association of the IRF6 rs2235371 and rs861019 Polymorphisms with Non-Syndromic Cleft Lip with or without Cleft Palate in the Yakut Population |
| title_full_unstemmed | Association of the IRF6 rs2235371 and rs861019 Polymorphisms with Non-Syndromic Cleft Lip with or without Cleft Palate in the Yakut Population |
| title_short | Association of the IRF6 rs2235371 and rs861019 Polymorphisms with Non-Syndromic Cleft Lip with or without Cleft Palate in the Yakut Population |
| title_sort | association of the irf6 rs2235371 and rs861019 polymorphisms with non syndromic cleft lip with or without cleft palate in the yakut population |
| topic | orofacial cleft irf6 gene yakuts haplotype diplotype rs2235371 rs861019 |
| url | http://ijbm.org/articles/i44/ijbm_11(4)_oa28.pdf |
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