Identification, characterization and expression analysis of biglycan in asian elephant (elephas maximus)
The aims of this study were to investigate the coding sequence and the deduced amino acid sequence of Asian elephant"s biglycan gene as well as its expression in different tissues and conditions using wound healing as a model. The results showed that Asian elephant biglycan coding sequence was...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2017-09-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2200 |
| _version_ | 1797800589165330432 |
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| author | Siriwadee CHOMDEJ Waraluk SAOKEAW Kittisak BUDDHACHAT Waranee PRADIT Puntita SIENGDEE Sittidet MAHASAWANGKUL Supaphen SRIPIBOON Chalermchart SOMGIRD Korakot NGANVONGPANIT Siriwan ONGCHAI Chatchote THITARAM |
| author_facet | Siriwadee CHOMDEJ Waraluk SAOKEAW Kittisak BUDDHACHAT Waranee PRADIT Puntita SIENGDEE Sittidet MAHASAWANGKUL Supaphen SRIPIBOON Chalermchart SOMGIRD Korakot NGANVONGPANIT Siriwan ONGCHAI Chatchote THITARAM |
| author_sort | Siriwadee CHOMDEJ |
| collection | DOAJ |
| description | The aims of this study were to investigate the coding sequence and the deduced amino acid sequence of Asian elephant"s biglycan gene as
well as its expression in different tissues and conditions using wound healing as a model. The results showed that Asian elephant biglycan
coding sequence was 1,110 base pair (bp) long (accession number: JQ753329), encoding 369 amino acids. The coding and amino acid
sequences between Asian and African elephants revealed 99% and 98% similarity, respectively. The conserved domains of biglycan protein
were also observed. In addition, its expression was found in 15 tissues with a predominant expression in cartilage and spleen. For expression
analysis in the wound healing process, it was found that the level of biglycan mRNA was influenced by many factors, including age, type of
wound and stage of wound healing. |
| first_indexed | 2024-03-13T04:37:37Z |
| format | Article |
| id | doaj.art-b7e5bdb930d649aa9cc4ea416354c326 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:37Z |
| publishDate | 2017-09-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-b7e5bdb930d649aa9cc4ea416354c3262023-06-19T06:45:03ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512017-09-01236997100110.9775/kvfd.2017.179452200Identification, characterization and expression analysis of biglycan in asian elephant (elephas maximus)Siriwadee CHOMDEJ0Waraluk SAOKEAW1Kittisak BUDDHACHAT2Waranee PRADIT3Puntita SIENGDEE4Sittidet MAHASAWANGKUL5Supaphen SRIPIBOON6Chalermchart SOMGIRD7Korakot NGANVONGPANIT8Siriwan ONGCHAI9Chatchote THITARAM10Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, THAILANDDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, THAILANDExcellent Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai, 50200 THAILANDExcellent Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai, 50200 THAILANDExcellent Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai, 50200 THAILANDNational Elephant Institute, Forest Industry Organization, Lampang, 52190, THAILANDCenter of Excellence in Elephant Research and Education, Chiang Mai University, Chiang Mai 50200, THAILANDCenter of Excellence in Elephant Research and Education, Chiang Mai University, Chiang Mai 50200, THAILANDAnimal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, THAILANDThailand Excellence Center for Tissue Engineering and Stem Cells and Center of Excellence for Innovation in Chemistry, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, THAILANDCenter of Excellence in Elephant Research and Education, Chiang Mai University, Chiang Mai 50200, THAILANDThe aims of this study were to investigate the coding sequence and the deduced amino acid sequence of Asian elephant"s biglycan gene as well as its expression in different tissues and conditions using wound healing as a model. The results showed that Asian elephant biglycan coding sequence was 1,110 base pair (bp) long (accession number: JQ753329), encoding 369 amino acids. The coding and amino acid sequences between Asian and African elephants revealed 99% and 98% similarity, respectively. The conserved domains of biglycan protein were also observed. In addition, its expression was found in 15 tissues with a predominant expression in cartilage and spleen. For expression analysis in the wound healing process, it was found that the level of biglycan mRNA was influenced by many factors, including age, type of wound and stage of wound healing.https://vetdergikafkas.org/pdf.php?id=2200asian elephantsbiglycangene expressionsequencingwound healing |
| spellingShingle | Siriwadee CHOMDEJ Waraluk SAOKEAW Kittisak BUDDHACHAT Waranee PRADIT Puntita SIENGDEE Sittidet MAHASAWANGKUL Supaphen SRIPIBOON Chalermchart SOMGIRD Korakot NGANVONGPANIT Siriwan ONGCHAI Chatchote THITARAM Identification, characterization and expression analysis of biglycan in asian elephant (elephas maximus) Kafkas Universitesi Veteriner Fakültesi Dergisi asian elephants biglycan gene expression sequencing wound healing |
| title | Identification, characterization and expression analysis of biglycan in asian elephant (elephas maximus) |
| title_full | Identification, characterization and expression analysis of biglycan in asian elephant (elephas maximus) |
| title_fullStr | Identification, characterization and expression analysis of biglycan in asian elephant (elephas maximus) |
| title_full_unstemmed | Identification, characterization and expression analysis of biglycan in asian elephant (elephas maximus) |
| title_short | Identification, characterization and expression analysis of biglycan in asian elephant (elephas maximus) |
| title_sort | identification characterization and expression analysis of biglycan in asian elephant elephas maximus |
| topic | asian elephants biglycan gene expression sequencing wound healing |
| url | https://vetdergikafkas.org/pdf.php?id=2200 |
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