Propolis Affects <i>Pseudomonas aeruginosa</i> Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols
<i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) is an opportunistic pathogen responsible for a wide range of clinical conditions, from mild infections to life-threatening nosocomial biofilm-associated diseases, which are particularly severe in susceptible individuals. Th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/2/243 |
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| author | Aida Meto Bruna Colombari Agron Meto Giorgia Boaretto Diego Pinetti Lucia Marchetti Stefania Benvenuti Federica Pellati Elisabetta Blasi |
| author_facet | Aida Meto Bruna Colombari Agron Meto Giorgia Boaretto Diego Pinetti Lucia Marchetti Stefania Benvenuti Federica Pellati Elisabetta Blasi |
| author_sort | Aida Meto |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) is an opportunistic pathogen responsible for a wide range of clinical conditions, from mild infections to life-threatening nosocomial biofilm-associated diseases, which are particularly severe in susceptible individuals. The aim of this <i>in vitro</i> study was to assess the effects of an Albanian propolis on several virulence-related factors of <i>P. aeruginosa</i>, such as growth ability, biofilm formation, extracellular DNA (eDNA) release and phenazine production. To this end, propolis was processed using three different solvents and the extracted polyphenolic compounds were identified by means of high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis. As assessed by a bioluminescence-based assay, among the three propolis extracts, the ethanol (EtOH) extract was the most effective in inhibiting both microbial growth and biofilm formation, followed by propylene glycol (PG) and polyethylene glycol 400 (PEG 400) propolis extracts. Furthermore, <i>Pseudomonas</i> exposure to propolis EtOH extract caused a decrease in eDNA release and phenazine production. Finally, caffeic acid phenethyl ester (CAPE) and quercetin decreased upon propolis EtOH extract exposure to bacteria. Overall, our data add new insights on the anti-microbial properties of a natural compound, such as propolis against <i>P. aeruginosa</i>. The potential implications of these findings will be discussed. |
| first_indexed | 2024-12-11T06:45:37Z |
| format | Article |
| id | doaj.art-b7e5e6dfcca945739b31b6e8469164e5 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-11T06:45:37Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b7e5e6dfcca945739b31b6e8469164e52022-12-22T01:17:06ZengMDPI AGMicroorganisms2076-26072020-02-018224310.3390/microorganisms8020243microorganisms8020243Propolis Affects <i>Pseudomonas aeruginosa</i> Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of PolyphenolsAida Meto0Bruna Colombari1Agron Meto2Giorgia Boaretto3Diego Pinetti4Lucia Marchetti5Stefania Benvenuti6Federica Pellati7Elisabetta Blasi8School of Doctorate in Clinical and Experimental Medicine, University of Modena and Reggio Emilia, Via G. Campi 287-103, 41125 Modena, ItalyDepartment of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, ItalyDepartment of Therapy, Faculty of Dental Medicine, University of Aldent, Rr. e Dibres 235, 1000 Tirana, AlbaniaSchool of Specialization in Microbiology and Virology, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, ItalyCentro Interdipartimentale Grandi Strumenti, University of Modena and Reggio Emilia, via Campi 213/A, 41125 Modena, ItalySchool of Doctorate in Clinical and Experimental Medicine, University of Modena and Reggio Emilia, Via G. Campi 287-103, 41125 Modena, ItalyDepartment of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, ItalyDepartment of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, ItalyDepartment of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy<i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) is an opportunistic pathogen responsible for a wide range of clinical conditions, from mild infections to life-threatening nosocomial biofilm-associated diseases, which are particularly severe in susceptible individuals. The aim of this <i>in vitro</i> study was to assess the effects of an Albanian propolis on several virulence-related factors of <i>P. aeruginosa</i>, such as growth ability, biofilm formation, extracellular DNA (eDNA) release and phenazine production. To this end, propolis was processed using three different solvents and the extracted polyphenolic compounds were identified by means of high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis. As assessed by a bioluminescence-based assay, among the three propolis extracts, the ethanol (EtOH) extract was the most effective in inhibiting both microbial growth and biofilm formation, followed by propylene glycol (PG) and polyethylene glycol 400 (PEG 400) propolis extracts. Furthermore, <i>Pseudomonas</i> exposure to propolis EtOH extract caused a decrease in eDNA release and phenazine production. Finally, caffeic acid phenethyl ester (CAPE) and quercetin decreased upon propolis EtOH extract exposure to bacteria. Overall, our data add new insights on the anti-microbial properties of a natural compound, such as propolis against <i>P. aeruginosa</i>. The potential implications of these findings will be discussed.https://www.mdpi.com/2076-2607/8/2/243<i>p. aeruginosa</i>virulence factorsphenazinesednamicantimicrobialantibiofilmpropolispolyphenols |
| spellingShingle | Aida Meto Bruna Colombari Agron Meto Giorgia Boaretto Diego Pinetti Lucia Marchetti Stefania Benvenuti Federica Pellati Elisabetta Blasi Propolis Affects <i>Pseudomonas aeruginosa</i> Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols Microorganisms <i>p. aeruginosa</i> virulence factors phenazines edna mic antimicrobial antibiofilm propolis polyphenols |
| title | Propolis Affects <i>Pseudomonas aeruginosa</i> Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols |
| title_full | Propolis Affects <i>Pseudomonas aeruginosa</i> Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols |
| title_fullStr | Propolis Affects <i>Pseudomonas aeruginosa</i> Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols |
| title_full_unstemmed | Propolis Affects <i>Pseudomonas aeruginosa</i> Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols |
| title_short | Propolis Affects <i>Pseudomonas aeruginosa</i> Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols |
| title_sort | propolis affects i pseudomonas aeruginosa i growth biofilm formation edna release and phenazine production potential involvement of polyphenols |
| topic | <i>p. aeruginosa</i> virulence factors phenazines edna mic antimicrobial antibiofilm propolis polyphenols |
| url | https://www.mdpi.com/2076-2607/8/2/243 |
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