Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa
Summary Microbes such as Pseudomonas aeruginosa are often challenged by rapidly changing nutritional environments. In order to adapt to these shifts in nutrient availability, bacteria exert tight transcriptional control over the enzymes of central metabolism. This transcriptional control is orchestr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13423 |
| _version_ | 1818500485727911936 |
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| author | Stephen K. Dolan Greicy Pereira Rafael Silva‐Rocha Martin Welch |
| author_facet | Stephen K. Dolan Greicy Pereira Rafael Silva‐Rocha Martin Welch |
| author_sort | Stephen K. Dolan |
| collection | DOAJ |
| description | Summary Microbes such as Pseudomonas aeruginosa are often challenged by rapidly changing nutritional environments. In order to adapt to these shifts in nutrient availability, bacteria exert tight transcriptional control over the enzymes of central metabolism. This transcriptional control is orchestrated by a series of transcriptional repressors and activators. Although a number of these transcription factors have been identified, many others remain uncharacterized. Here, we present a simple pipeline to uncover and validate the targets of uncharacterized transcriptional regulators in P. aeruginosa. We use this approach to identify and confirm that an orthologue of the Pseudomonas fluorescens transcriptional regulator (RccR) binds to the upstream region of isocitrate lyase (aceA) in P. aeruginosa, thereby repressing flux through the glyoxylate shunt during growth on non‐C2 carbon sources. |
| first_indexed | 2024-12-10T20:43:21Z |
| format | Article |
| id | doaj.art-b7e6b5791d5744e5a02dd93133d1cd32 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-10T20:43:21Z |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-b7e6b5791d5744e5a02dd93133d1cd322022-12-22T01:34:18ZengWileyMicrobial Biotechnology1751-79152020-01-0113128528910.1111/1751-7915.13423Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosaStephen K. Dolan0Greicy Pereira1Rafael Silva‐Rocha2Martin Welch3Department of Biochemistry University of Cambridge Cambridge UKFaculdade de Medicina de Ribeirão Preto Universidade de São Paulo Ribeirão Preto BrazilFaculdade de Medicina de Ribeirão Preto Universidade de São Paulo Ribeirão Preto BrazilDepartment of Biochemistry University of Cambridge Cambridge UKSummary Microbes such as Pseudomonas aeruginosa are often challenged by rapidly changing nutritional environments. In order to adapt to these shifts in nutrient availability, bacteria exert tight transcriptional control over the enzymes of central metabolism. This transcriptional control is orchestrated by a series of transcriptional repressors and activators. Although a number of these transcription factors have been identified, many others remain uncharacterized. Here, we present a simple pipeline to uncover and validate the targets of uncharacterized transcriptional regulators in P. aeruginosa. We use this approach to identify and confirm that an orthologue of the Pseudomonas fluorescens transcriptional regulator (RccR) binds to the upstream region of isocitrate lyase (aceA) in P. aeruginosa, thereby repressing flux through the glyoxylate shunt during growth on non‐C2 carbon sources.https://doi.org/10.1111/1751-7915.13423 |
| spellingShingle | Stephen K. Dolan Greicy Pereira Rafael Silva‐Rocha Martin Welch Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa Microbial Biotechnology |
| title | Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa |
| title_full | Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa |
| title_fullStr | Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa |
| title_full_unstemmed | Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa |
| title_short | Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa |
| title_sort | transcriptional regulation of central carbon metabolism in pseudomonas aeruginosa |
| url | https://doi.org/10.1111/1751-7915.13423 |
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