Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs
Introduction/Aim. Pseudomonas aeruginosa (P. aeruginosa) is the most common cause of wound infections, following the disruption of the skin or mucous membranes integrity. The aim of this study was to analyze of the presence P. aeruginosa in wound swabs, antibiotics susceptibility testing, d...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Military Health Department, Ministry of Defance, Serbia
2015-01-01
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| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2015/0042-84501500108S.pdf |
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| author | Stanković-Nedeljković Nataša Tiodorović Branislav Kocić Branislava Ćirić Vojislav Milojković Marko Waisi Hadi |
| author_facet | Stanković-Nedeljković Nataša Tiodorović Branislav Kocić Branislava Ćirić Vojislav Milojković Marko Waisi Hadi |
| author_sort | Stanković-Nedeljković Nataša |
| collection | DOAJ |
| description | Introduction/Aim. Pseudomonas aeruginosa (P. aeruginosa) is the most common
cause of wound infections, following the disruption of the skin or mucous
membranes integrity. The aim of this study was to analyze of the presence P.
aeruginosa in wound swabs, antibiotics susceptibility testing, determination
of the minimum inhibitory concentrations (MICs) of antibiotics, testing of
the metallo-β-lactamases (MBLs) production, isolates serotyping and analysis
of the most common serotypes resistance. Methods. A total of 90 outpatients
and 55 intpatients wound swabs were cultivated. Wound swabs were taken from
the patients with wound infections symptoms. Antibiotics susceptibility
testing was performed to: meropenem, imipenem, piperacillin-tazobactam,
ceftazidime, cefepime, amikacin, gentamicin, netilmicin, ofloxacin,
ciprofloxacin and colistin (HiMedia). Polyvalent and monovalent antisera for
agglutination (Biorad) were used in P. aeruginosa agglutination. Results. P.
aeruginosa was isolated from 36.55% wound swabs (36.66% of the inpatients
wounds and 36.36% of the outpatients). The analyzed isolates showed the
highest degree of sensitivity to colistin (100%) and meropenem (93.44%) and
the lowest to cefepime (19.54%). The majority of the inpatients isolates had
12 μg/mL (28.57%) MIC for piperacillin-tazobactam and 16 μg/mL (28.57%) for
the outpatients. The most common MICs for ciprofloxacin were 0.19 μg/mL
(31.81%) for the nosocomial isolates, and 0.25 μg/mL (28.57%) for the
outpatients’ ones. The most common MICs for amikacin of the nosocomial
isolates were 6 μg/ml (40.9%), and for the outpatients ones 4 μg/mL (33.33%).
Five (9.43%) isolates produced MBLs. The most common serotypes were P11
(22.64%), P6 (15.09%) and P1 (11.32%). Conclusion. Neither the increased
presence of P. aeruginosa was noticed in wounds swabs, nor the antibiotic
resistance in the nosocomial isolates compared to those from outpatients. The
analyzed isolates had the higest sensitivity to colistin and meropenem, and
the lowest to cefepime. [Projekat Ministarstva nauke Republike Srbije, br. TR
31080] |
| first_indexed | 2024-12-10T17:31:06Z |
| format | Article |
| id | doaj.art-b7e81931103d4dfaadb03a638c571c67 |
| institution | Directory Open Access Journal |
| issn | 0042-8450 2406-0720 |
| language | English |
| last_indexed | 2024-12-10T17:31:06Z |
| publishDate | 2015-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-b7e81931103d4dfaadb03a638c571c672022-12-22T01:39:41ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502406-07202015-01-017211996100310.2298/VSP131224108S0042-84501500108SPseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabsStanković-Nedeljković Nataša0Tiodorović Branislav1Kocić Branislava2Ćirić Vojislav3Milojković Marko4Waisi Hadi5Health Center "Aleksinac", AleksinacFaculty of Medicine, NišFaculty of Medicine, NišClinical Center, Department of Endocrinology, NišSchool of Agriculture “Šumatovac”, AleksinacInstitute for Water Resources "Jaroslav Černi", BelgradeIntroduction/Aim. Pseudomonas aeruginosa (P. aeruginosa) is the most common cause of wound infections, following the disruption of the skin or mucous membranes integrity. The aim of this study was to analyze of the presence P. aeruginosa in wound swabs, antibiotics susceptibility testing, determination of the minimum inhibitory concentrations (MICs) of antibiotics, testing of the metallo-β-lactamases (MBLs) production, isolates serotyping and analysis of the most common serotypes resistance. Methods. A total of 90 outpatients and 55 intpatients wound swabs were cultivated. Wound swabs were taken from the patients with wound infections symptoms. Antibiotics susceptibility testing was performed to: meropenem, imipenem, piperacillin-tazobactam, ceftazidime, cefepime, amikacin, gentamicin, netilmicin, ofloxacin, ciprofloxacin and colistin (HiMedia). Polyvalent and monovalent antisera for agglutination (Biorad) were used in P. aeruginosa agglutination. Results. P. aeruginosa was isolated from 36.55% wound swabs (36.66% of the inpatients wounds and 36.36% of the outpatients). The analyzed isolates showed the highest degree of sensitivity to colistin (100%) and meropenem (93.44%) and the lowest to cefepime (19.54%). The majority of the inpatients isolates had 12 μg/mL (28.57%) MIC for piperacillin-tazobactam and 16 μg/mL (28.57%) for the outpatients. The most common MICs for ciprofloxacin were 0.19 μg/mL (31.81%) for the nosocomial isolates, and 0.25 μg/mL (28.57%) for the outpatients’ ones. The most common MICs for amikacin of the nosocomial isolates were 6 μg/ml (40.9%), and for the outpatients ones 4 μg/mL (33.33%). Five (9.43%) isolates produced MBLs. The most common serotypes were P11 (22.64%), P6 (15.09%) and P1 (11.32%). Conclusion. Neither the increased presence of P. aeruginosa was noticed in wounds swabs, nor the antibiotic resistance in the nosocomial isolates compared to those from outpatients. The analyzed isolates had the higest sensitivity to colistin and meropenem, and the lowest to cefepime. [Projekat Ministarstva nauke Republike Srbije, br. TR 31080]http://www.doiserbia.nb.rs/img/doi/0042-8450/2015/0042-84501500108S.pdfpseudomonas aeruginosawounds infectionserotypinganti-bacterial agentsdrug resistance |
| spellingShingle | Stanković-Nedeljković Nataša Tiodorović Branislav Kocić Branislava Ćirić Vojislav Milojković Marko Waisi Hadi Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs Vojnosanitetski Pregled pseudomonas aeruginosa wounds infection serotyping anti-bacterial agents drug resistance |
| title | Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs |
| title_full | Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs |
| title_fullStr | Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs |
| title_full_unstemmed | Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs |
| title_short | Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs |
| title_sort | pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs |
| topic | pseudomonas aeruginosa wounds infection serotyping anti-bacterial agents drug resistance |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2015/0042-84501500108S.pdf |
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