Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization
The efficient uptake is important for the xylose utilization by Saccharomyces cerevisiae. A heterogenous transporter Mgt05196p was cloned from Meyerozyma guilliermondii and expressed in Saccharomyces cerevisiae [1]. This data article contains the transport characteristics of Mgt05196p in S. cerevisi...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-09-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340915000736 |
| _version_ | 1818172306673893376 |
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| author | Chengqiang Wang Xiaoming Bao Yanwei Li Chunlei Jiao Jin Hou Qingzhu Zhang Weixin Zhang Weifeng Liu Yu Shen |
| author_facet | Chengqiang Wang Xiaoming Bao Yanwei Li Chunlei Jiao Jin Hou Qingzhu Zhang Weixin Zhang Weifeng Liu Yu Shen |
| author_sort | Chengqiang Wang |
| collection | DOAJ |
| description | The efficient uptake is important for the xylose utilization by Saccharomyces cerevisiae. A heterogenous transporter Mgt05196p was cloned from Meyerozyma guilliermondii and expressed in Saccharomyces cerevisiae [1]. This data article contains the transport characteristics of Mgt05196p in S. cerevisiae. The fluorescence of fusion protein Mgt05196p-GFP expressing strain was located on the cell surface demonstrated that the heterogenous transporter Mgt05196p was targeted to the plasma membrane of S. cerevisiae. The expressing of Mgt05196p in the hxt null S. cerevisiae endowed the strain with the glucose and d-xylose absorption capacity, as well as expressing the native d-xylose transporter Gal2p. The transmembrane domains of Mgt05196p were predicted and compared with the XylEp, whose crystal structure was revealed. And then, the homologous modeling of Mgt05196p was built basing on the XylEp to find out the crucial amino acid residues for sugars binding and transport. |
| first_indexed | 2024-12-11T19:10:31Z |
| format | Article |
| id | doaj.art-b7eb6f67572c493b9d95074a74b36c72 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-11T19:10:31Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-b7eb6f67572c493b9d95074a74b36c722022-12-22T00:53:48ZengElsevierData in Brief2352-34092015-09-014C11912610.1016/j.dib.2015.05.005Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilizationChengqiang Wang0Xiaoming Bao1Yanwei Li2Chunlei Jiao3Jin Hou4Qingzhu Zhang5Weixin Zhang6Weifeng Liu7Yu Shen8The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR ChinaThe State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR ChinaEnvironment Research Institute, Shandong University, Shan Da Nan Road 27, Jinan 250100, PR ChinaThe State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR ChinaThe State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR ChinaEnvironment Research Institute, Shandong University, Shan Da Nan Road 27, Jinan 250100, PR ChinaThe State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR ChinaThe State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR ChinaThe State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road27, Jinan 250100, PR ChinaThe efficient uptake is important for the xylose utilization by Saccharomyces cerevisiae. A heterogenous transporter Mgt05196p was cloned from Meyerozyma guilliermondii and expressed in Saccharomyces cerevisiae [1]. This data article contains the transport characteristics of Mgt05196p in S. cerevisiae. The fluorescence of fusion protein Mgt05196p-GFP expressing strain was located on the cell surface demonstrated that the heterogenous transporter Mgt05196p was targeted to the plasma membrane of S. cerevisiae. The expressing of Mgt05196p in the hxt null S. cerevisiae endowed the strain with the glucose and d-xylose absorption capacity, as well as expressing the native d-xylose transporter Gal2p. The transmembrane domains of Mgt05196p were predicted and compared with the XylEp, whose crystal structure was revealed. And then, the homologous modeling of Mgt05196p was built basing on the XylEp to find out the crucial amino acid residues for sugars binding and transport.http://www.sciencedirect.com/science/article/pii/S2352340915000736 |
| spellingShingle | Chengqiang Wang Xiaoming Bao Yanwei Li Chunlei Jiao Jin Hou Qingzhu Zhang Weixin Zhang Weifeng Liu Yu Shen Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization Data in Brief |
| title | Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization |
| title_full | Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization |
| title_fullStr | Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization |
| title_full_unstemmed | Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization |
| title_short | Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization |
| title_sort | data set for cloning and characterization of heterologous transporters in saccharomyces cerevisiae and identification of important amino acids for xylose utilization |
| url | http://www.sciencedirect.com/science/article/pii/S2352340915000736 |
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