Effective Decolorization and Detoxification of Single and Mixed Dyes with Crude Laccase Preparation from a White-Rot Fungus Strain <i>Pleurotus eryngii</i>
To fully harness the potential of laccase in the efficient decolorization and detoxification of single and mixed dyes with diverse chemical structures, we carried out a systematic study on the decolorization and detoxification of single and mixed dyes using a crude laccase preparation obtained from...
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MDPI AG
2024-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/3/669 |
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| author | Mingrui Ge Wei Deng Ziyi Wang Chenwen Weng Yang Yang |
| author_facet | Mingrui Ge Wei Deng Ziyi Wang Chenwen Weng Yang Yang |
| author_sort | Mingrui Ge |
| collection | DOAJ |
| description | To fully harness the potential of laccase in the efficient decolorization and detoxification of single and mixed dyes with diverse chemical structures, we carried out a systematic study on the decolorization and detoxification of single and mixed dyes using a crude laccase preparation obtained from a white-rot fungus strain, <i>Pleurotus eryngii</i>. The crude laccase preparation showed efficient decolorization of azo, anthraquinone, triphenylmethane, and indigo dyes, and the reaction rate constants followed the order Remazol Brilliant Blue R > Bromophenol blue > Indigo carmine > New Coccine > Reactive Blue 4 > Reactive Black 5 > Acid Orange 7 > Methyl green. This laccase preparation exhibited notable tolerance to SO<sub>4</sub><sup>2−</sup> salts such as MnSO<sub>4</sub>, MgSO<sub>4</sub>, ZnSO<sub>4</sub>, Na<sub>2</sub>SO<sub>4</sub>, K<sub>2</sub>SO<sub>4</sub>, and CdSO<sub>4</sub> during the decolorization of various types of dyes, but was significantly inhibited by Cl<sup>−</sup> salts. Additionally, this laccase preparation demonstrated strong tolerance to some organic solvents such as glycerol, ethylene glycol, propanediol, and butanediol. The crude laccase preparation demonstrated the efficient decolorization of dye mixtures, including azo + azo, azo + anthraquinone, azo + triphenylmethane, anthraquinone + indigo, anthraquinone + triphenylmethane, and indigo + triphenylmethane dyes. The decolorization kinetics of mixed dyes provided preliminary insight into the interactions between dyes in the decolorization process of mixed dyes, and the underlying reasons and mechanisms were discussed. Importantly, the crude laccase from <i>Pleurotus eryngii</i> showed efficient repeated-batch decolorization of single-, two-, and four-dye mixtures. This crude laccase demonstrated high stability and reusability in repeated-batch decolorization. Furthermore, this crude laccase was efficient in the detoxification of different types of single dyes and mixed dyes containing different types of dyes, and the phytotoxicity of decolorized dyes (single and mixed dyes) was significantly reduced. The crude laccase efficiently eliminated phytotoxicity associated with single and mixed dyes. Consequently, the crude laccase from <i>Pleurotus eryngii</i> offers significant potential for practical applications in the efficient decolorization and management of single and mixed dye pollutants with different chemical structures. |
| first_indexed | 2024-03-08T03:52:05Z |
| format | Article |
| id | doaj.art-b7ecacbc09f3421dbccfdb560c57a421 |
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| last_indexed | 2024-03-08T03:52:05Z |
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| spelling | doaj.art-b7ecacbc09f3421dbccfdb560c57a4212024-02-09T15:19:02ZengMDPI AGMolecules1420-30492024-01-0129366910.3390/molecules29030669Effective Decolorization and Detoxification of Single and Mixed Dyes with Crude Laccase Preparation from a White-Rot Fungus Strain <i>Pleurotus eryngii</i>Mingrui Ge0Wei Deng1Ziyi Wang2Chenwen Weng3Yang Yang4School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, ChinaSchool of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, ChinaSchool of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, ChinaSchool of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, ChinaSchool of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, ChinaTo fully harness the potential of laccase in the efficient decolorization and detoxification of single and mixed dyes with diverse chemical structures, we carried out a systematic study on the decolorization and detoxification of single and mixed dyes using a crude laccase preparation obtained from a white-rot fungus strain, <i>Pleurotus eryngii</i>. The crude laccase preparation showed efficient decolorization of azo, anthraquinone, triphenylmethane, and indigo dyes, and the reaction rate constants followed the order Remazol Brilliant Blue R > Bromophenol blue > Indigo carmine > New Coccine > Reactive Blue 4 > Reactive Black 5 > Acid Orange 7 > Methyl green. This laccase preparation exhibited notable tolerance to SO<sub>4</sub><sup>2−</sup> salts such as MnSO<sub>4</sub>, MgSO<sub>4</sub>, ZnSO<sub>4</sub>, Na<sub>2</sub>SO<sub>4</sub>, K<sub>2</sub>SO<sub>4</sub>, and CdSO<sub>4</sub> during the decolorization of various types of dyes, but was significantly inhibited by Cl<sup>−</sup> salts. Additionally, this laccase preparation demonstrated strong tolerance to some organic solvents such as glycerol, ethylene glycol, propanediol, and butanediol. The crude laccase preparation demonstrated the efficient decolorization of dye mixtures, including azo + azo, azo + anthraquinone, azo + triphenylmethane, anthraquinone + indigo, anthraquinone + triphenylmethane, and indigo + triphenylmethane dyes. The decolorization kinetics of mixed dyes provided preliminary insight into the interactions between dyes in the decolorization process of mixed dyes, and the underlying reasons and mechanisms were discussed. Importantly, the crude laccase from <i>Pleurotus eryngii</i> showed efficient repeated-batch decolorization of single-, two-, and four-dye mixtures. This crude laccase demonstrated high stability and reusability in repeated-batch decolorization. Furthermore, this crude laccase was efficient in the detoxification of different types of single dyes and mixed dyes containing different types of dyes, and the phytotoxicity of decolorized dyes (single and mixed dyes) was significantly reduced. The crude laccase efficiently eliminated phytotoxicity associated with single and mixed dyes. Consequently, the crude laccase from <i>Pleurotus eryngii</i> offers significant potential for practical applications in the efficient decolorization and management of single and mixed dye pollutants with different chemical structures.https://www.mdpi.com/1420-3049/29/3/669white-rot fungilaccasedyesdye mixturesdecolorizationrepeated-batch decolorization |
| spellingShingle | Mingrui Ge Wei Deng Ziyi Wang Chenwen Weng Yang Yang Effective Decolorization and Detoxification of Single and Mixed Dyes with Crude Laccase Preparation from a White-Rot Fungus Strain <i>Pleurotus eryngii</i> Molecules white-rot fungi laccase dyes dye mixtures decolorization repeated-batch decolorization |
| title | Effective Decolorization and Detoxification of Single and Mixed Dyes with Crude Laccase Preparation from a White-Rot Fungus Strain <i>Pleurotus eryngii</i> |
| title_full | Effective Decolorization and Detoxification of Single and Mixed Dyes with Crude Laccase Preparation from a White-Rot Fungus Strain <i>Pleurotus eryngii</i> |
| title_fullStr | Effective Decolorization and Detoxification of Single and Mixed Dyes with Crude Laccase Preparation from a White-Rot Fungus Strain <i>Pleurotus eryngii</i> |
| title_full_unstemmed | Effective Decolorization and Detoxification of Single and Mixed Dyes with Crude Laccase Preparation from a White-Rot Fungus Strain <i>Pleurotus eryngii</i> |
| title_short | Effective Decolorization and Detoxification of Single and Mixed Dyes with Crude Laccase Preparation from a White-Rot Fungus Strain <i>Pleurotus eryngii</i> |
| title_sort | effective decolorization and detoxification of single and mixed dyes with crude laccase preparation from a white rot fungus strain i pleurotus eryngii i |
| topic | white-rot fungi laccase dyes dye mixtures decolorization repeated-batch decolorization |
| url | https://www.mdpi.com/1420-3049/29/3/669 |
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