Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres
Abstract An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) usin...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-08-01
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| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s11671-017-2236-0 |
| _version_ | 1797716206320353280 |
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| author | Mahbubur Rahman Lee Yook Heng Dedi Futra Tan Ling Ling |
| author_facet | Mahbubur Rahman Lee Yook Heng Dedi Futra Tan Ling Ling |
| author_sort | Mahbubur Rahman |
| collection | DOAJ |
| description | Abstract An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) using an electroactive anthraquninone oligonucleotide label was used for measuring the biosensor response. Loading of gold nanoparticles (AuNPs) on the DNA-latex particle electrode has significantly amplified the faradaic current of DNA hybridisation. Together with the use of a reported probe, the biosensor has demonstrated high sensitivity. The DNA biosensor yielded a reproducible and wide linear response range to target DNA from 1.0 × 10−21 to 1.0 × 10−8 M (relative standard deviation, RSD = 4.5%, n = 5) with a limit of detection (LOD) of 1.0 × 10−21 M (R 2 = 0.99). The biosensor obtained satisfactory recovery values between 91 and 109% (n = 3) for the detection of V. cholerae DNA in spiked samples and could be reused for six consecutive DNA assays with a repeatability RSD value of 5% (n = 5). The electrochemical biosensor response was stable and maintainable at 95% of its original response up to 58 days of storage period. |
| first_indexed | 2024-03-12T08:18:07Z |
| format | Article |
| id | doaj.art-b7f038e241174ae999e8e51381cd1ad6 |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T08:18:07Z |
| publishDate | 2017-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-b7f038e241174ae999e8e51381cd1ad62023-09-02T18:44:42ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2017-08-0112111210.1186/s11671-017-2236-0Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite NanospheresMahbubur Rahman0Lee Yook Heng1Dedi Futra2Tan Ling Ling3Department of General Educational Development (GED), Faculty of Science and Information Technology, Daffodil International UniversitySchool of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan MalaysiaDepartment of Chemistry Education, Faculty of Education, Graduate Program, University RiauSoutheast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute For Environment and Development (LESTARI), University Kebangsaan MalaysiaAbstract An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) using an electroactive anthraquninone oligonucleotide label was used for measuring the biosensor response. Loading of gold nanoparticles (AuNPs) on the DNA-latex particle electrode has significantly amplified the faradaic current of DNA hybridisation. Together with the use of a reported probe, the biosensor has demonstrated high sensitivity. The DNA biosensor yielded a reproducible and wide linear response range to target DNA from 1.0 × 10−21 to 1.0 × 10−8 M (relative standard deviation, RSD = 4.5%, n = 5) with a limit of detection (LOD) of 1.0 × 10−21 M (R 2 = 0.99). The biosensor obtained satisfactory recovery values between 91 and 109% (n = 3) for the detection of V. cholerae DNA in spiked samples and could be reused for six consecutive DNA assays with a repeatability RSD value of 5% (n = 5). The electrochemical biosensor response was stable and maintainable at 95% of its original response up to 58 days of storage period.http://link.springer.com/article/10.1186/s11671-017-2236-0Vibrio choleraePolystyrene co-acrylic acid (PSA) particlesLatex-gold nanosphereBiosensorsElectrochemical determinationEDC/NHS chemistry |
| spellingShingle | Mahbubur Rahman Lee Yook Heng Dedi Futra Tan Ling Ling Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres Nanoscale Research Letters Vibrio cholerae Polystyrene co-acrylic acid (PSA) particles Latex-gold nanosphere Biosensors Electrochemical determination EDC/NHS chemistry |
| title | Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres |
| title_full | Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres |
| title_fullStr | Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres |
| title_full_unstemmed | Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres |
| title_short | Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres |
| title_sort | ultrasensitive biosensor for the detection of vibrio cholerae dna with polystyrene co acrylic acid composite nanospheres |
| topic | Vibrio cholerae Polystyrene co-acrylic acid (PSA) particles Latex-gold nanosphere Biosensors Electrochemical determination EDC/NHS chemistry |
| url | http://link.springer.com/article/10.1186/s11671-017-2236-0 |
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