Biochemical and Expression Analyses Revealed the Involvement of Proanthocyanidins and/or Their Derivatives in Fiber Pigmentation of <i>Gossypium stocksii</i>
The wild cotton species <i>Gossypium stocksii</i> produces a brown fiber that provides a valuable resource for the color improvement of naturally colored cotton (NCC) fiber. However, the biochemical basis and molecular mechanism of its fiber pigmentation remain unclear. Herein, we analyz...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/2/1008 |
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| author | Yujie Sun Diandian Zhang Hongli Zheng Yuqing Wu Jun Mei Liping Ke Dongliang Yu Yuqiang Sun |
| author_facet | Yujie Sun Diandian Zhang Hongli Zheng Yuqing Wu Jun Mei Liping Ke Dongliang Yu Yuqiang Sun |
| author_sort | Yujie Sun |
| collection | DOAJ |
| description | The wild cotton species <i>Gossypium stocksii</i> produces a brown fiber that provides a valuable resource for the color improvement of naturally colored cotton (NCC) fiber. However, the biochemical basis and molecular mechanism of its fiber pigmentation remain unclear. Herein, we analyzed the dynamics of proanthocyanidins (PAs) accumulation in developing the fiber of <i>G. stocksii</i>, which suggested a similar role of PAs and/or their derivatives in the fiber coloration of <i>G. stocksii</i>. In addition, comparative transcriptomics analyses revealed that the PA biosynthetic genes were expressed at higher levels and for a longer period in developing fibers of <i>G. stocksii </i>than<i> G. arboreum </i>(white fiber), and the transcription factors, such as TT8, possibly played crucial regulatory roles in regulating the PA branch genes. Moreover, we found that the anthocyanidin reductase (ANR) was expressed at a higher level than the leucoanthocyanidin reductases (LARs) and significantly upregulated during fiber elongation, suggesting a major role of ANR in PA synthesis in <i>G. stocksii </i>fiber. In summary, this work revealed the accumulation of PAs and the expression enhancement of PA biosynthetic genes in developing fibers of <i>G. stocksii</i>. We believe this work will help our understanding of the molecular mechanisms of cotton fiber coloration and further promote the future breeding of novel NCCs. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:16:24Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b7f27f660c02465f9b463e26fd119df82023-11-23T14:08:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01232100810.3390/ijms23021008Biochemical and Expression Analyses Revealed the Involvement of Proanthocyanidins and/or Their Derivatives in Fiber Pigmentation of <i>Gossypium stocksii</i>Yujie Sun0Diandian Zhang1Hongli Zheng2Yuqing Wu3Jun Mei4Liping Ke5Dongliang Yu6Yuqiang Sun7Plant Genomics & Molecular Improvement of Colored Fiber Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaPlant Genomics & Molecular Improvement of Colored Fiber Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaPlant Genomics & Molecular Improvement of Colored Fiber Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaPlant Genomics & Molecular Improvement of Colored Fiber Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaPlant Genomics & Molecular Improvement of Colored Fiber Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaPlant Genomics & Molecular Improvement of Colored Fiber Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaPlant Genomics & Molecular Improvement of Colored Fiber Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaPlant Genomics & Molecular Improvement of Colored Fiber Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaThe wild cotton species <i>Gossypium stocksii</i> produces a brown fiber that provides a valuable resource for the color improvement of naturally colored cotton (NCC) fiber. However, the biochemical basis and molecular mechanism of its fiber pigmentation remain unclear. Herein, we analyzed the dynamics of proanthocyanidins (PAs) accumulation in developing the fiber of <i>G. stocksii</i>, which suggested a similar role of PAs and/or their derivatives in the fiber coloration of <i>G. stocksii</i>. In addition, comparative transcriptomics analyses revealed that the PA biosynthetic genes were expressed at higher levels and for a longer period in developing fibers of <i>G. stocksii </i>than<i> G. arboreum </i>(white fiber), and the transcription factors, such as TT8, possibly played crucial regulatory roles in regulating the PA branch genes. Moreover, we found that the anthocyanidin reductase (ANR) was expressed at a higher level than the leucoanthocyanidin reductases (LARs) and significantly upregulated during fiber elongation, suggesting a major role of ANR in PA synthesis in <i>G. stocksii </i>fiber. In summary, this work revealed the accumulation of PAs and the expression enhancement of PA biosynthetic genes in developing fibers of <i>G. stocksii</i>. We believe this work will help our understanding of the molecular mechanisms of cotton fiber coloration and further promote the future breeding of novel NCCs.https://www.mdpi.com/1422-0067/23/2/1008wild cottonbrown fiberproanthocyanidinTT8ANR |
| spellingShingle | Yujie Sun Diandian Zhang Hongli Zheng Yuqing Wu Jun Mei Liping Ke Dongliang Yu Yuqiang Sun Biochemical and Expression Analyses Revealed the Involvement of Proanthocyanidins and/or Their Derivatives in Fiber Pigmentation of <i>Gossypium stocksii</i> International Journal of Molecular Sciences wild cotton brown fiber proanthocyanidin TT8 ANR |
| title | Biochemical and Expression Analyses Revealed the Involvement of Proanthocyanidins and/or Their Derivatives in Fiber Pigmentation of <i>Gossypium stocksii</i> |
| title_full | Biochemical and Expression Analyses Revealed the Involvement of Proanthocyanidins and/or Their Derivatives in Fiber Pigmentation of <i>Gossypium stocksii</i> |
| title_fullStr | Biochemical and Expression Analyses Revealed the Involvement of Proanthocyanidins and/or Their Derivatives in Fiber Pigmentation of <i>Gossypium stocksii</i> |
| title_full_unstemmed | Biochemical and Expression Analyses Revealed the Involvement of Proanthocyanidins and/or Their Derivatives in Fiber Pigmentation of <i>Gossypium stocksii</i> |
| title_short | Biochemical and Expression Analyses Revealed the Involvement of Proanthocyanidins and/or Their Derivatives in Fiber Pigmentation of <i>Gossypium stocksii</i> |
| title_sort | biochemical and expression analyses revealed the involvement of proanthocyanidins and or their derivatives in fiber pigmentation of i gossypium stocksii i |
| topic | wild cotton brown fiber proanthocyanidin TT8 ANR |
| url | https://www.mdpi.com/1422-0067/23/2/1008 |
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