Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring
Background: Sulphur-oxidizing microorganisms are widely used in the biofiltration of total reduced sulphur compounds (odorous and neurotoxic) produced by industries such as the cellulose and petrochemical industries, which include high-temperature process steps. Some hyperthermophilic microorganisms...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-01-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345816301270 |
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| author | Emky Valdebenito-Rolack Nathaly Ruiz-Tagle Leslie Abarzúa Germán Aroca Homero Urrutia |
| author_facet | Emky Valdebenito-Rolack Nathaly Ruiz-Tagle Leslie Abarzúa Germán Aroca Homero Urrutia |
| author_sort | Emky Valdebenito-Rolack |
| collection | DOAJ |
| description | Background: Sulphur-oxidizing microorganisms are widely used in the biofiltration of total reduced sulphur compounds (odorous and neurotoxic) produced by industries such as the cellulose and petrochemical industries, which include high-temperature process steps. Some hyperthermophilic microorganisms have the capability to oxidize these compounds at high temperatures (>60°C), and archaea of this group, for example, Sulfolobus metallicus, are commonly used in biofiltration technology.
Results: In this study, a hyperthermophilic sulphur-oxidizing strain of archaea was isolated from a hot spring (Chillán, Chile) and designated as M1. It was identified as archaea of the genus Sulfolobus (99% homology with S. solfataricus 16S rDNA). Biofilms of this culture grown on polyethylene rings showed an elemental sulphur oxidation rate of 95.15 ± 15.39 mg S l-1 d-1, higher than the rate exhibited by the biofilm of the sulphur-oxidizing archaea S. metallicus (56.8 ± 10.91 mg l-1 d-1).
Conclusions: The results suggest that the culture M1 is useful for the biofiltration of total reduced sulphur gases at high temperatures and for other biotechnological applications. |
| first_indexed | 2024-04-13T08:07:20Z |
| format | Article |
| id | doaj.art-b7f4c4de4865454fb9bfd8b2433c9959 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-04-13T08:07:20Z |
| publishDate | 2017-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-b7f4c4de4865454fb9bfd8b2433c99592022-12-22T02:55:08ZengElsevierElectronic Journal of Biotechnology0717-34582017-01-0125C586310.1016/j.ejbt.2016.11.005Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot springEmky Valdebenito-Rolack0Nathaly Ruiz-Tagle1Leslie Abarzúa2Germán Aroca3Homero Urrutia4Laboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, ChileLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, ChileLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, ChileEscuela de Ingeniería Bioquímica, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, Valparaíso, ChileLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, ChileBackground: Sulphur-oxidizing microorganisms are widely used in the biofiltration of total reduced sulphur compounds (odorous and neurotoxic) produced by industries such as the cellulose and petrochemical industries, which include high-temperature process steps. Some hyperthermophilic microorganisms have the capability to oxidize these compounds at high temperatures (>60°C), and archaea of this group, for example, Sulfolobus metallicus, are commonly used in biofiltration technology. Results: In this study, a hyperthermophilic sulphur-oxidizing strain of archaea was isolated from a hot spring (Chillán, Chile) and designated as M1. It was identified as archaea of the genus Sulfolobus (99% homology with S. solfataricus 16S rDNA). Biofilms of this culture grown on polyethylene rings showed an elemental sulphur oxidation rate of 95.15 ± 15.39 mg S l-1 d-1, higher than the rate exhibited by the biofilm of the sulphur-oxidizing archaea S. metallicus (56.8 ± 10.91 mg l-1 d-1). Conclusions: The results suggest that the culture M1 is useful for the biofiltration of total reduced sulphur gases at high temperatures and for other biotechnological applications.http://www.sciencedirect.com/science/article/pii/S0717345816301270Biofilms on polyethyleneBiofiltrationCellulose industriesDenaturing gradient gel electrophoresis extremophileHyperthermophileIndustrial gas emissionsPetroleum refinerySulfolobusSulphideSulphur-oxidizing archaeaSulphur-oxidizing microorganisms |
| spellingShingle | Emky Valdebenito-Rolack Nathaly Ruiz-Tagle Leslie Abarzúa Germán Aroca Homero Urrutia Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring Electronic Journal of Biotechnology Biofilms on polyethylene Biofiltration Cellulose industries Denaturing gradient gel electrophoresis extremophile Hyperthermophile Industrial gas emissions Petroleum refinery Sulfolobus Sulphide Sulphur-oxidizing archaea Sulphur-oxidizing microorganisms |
| title | Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring |
| title_full | Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring |
| title_fullStr | Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring |
| title_full_unstemmed | Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring |
| title_short | Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring |
| title_sort | characterization of a hyperthermophilic sulphur oxidizing biofilm produced by archaea isolated from a hot spring |
| topic | Biofilms on polyethylene Biofiltration Cellulose industries Denaturing gradient gel electrophoresis extremophile Hyperthermophile Industrial gas emissions Petroleum refinery Sulfolobus Sulphide Sulphur-oxidizing archaea Sulphur-oxidizing microorganisms |
| url | http://www.sciencedirect.com/science/article/pii/S0717345816301270 |
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