Assessment of Antimicrobial Efficiency of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> Essential Oils against Spoilage and Pathogenic Microbes in Ice Cream and Fruit Juices
Nowadays, the use of antimicrobial natural agents as alternative food preservatives represents an intriguing case. The purpose of this study was to investigate possible antimicrobial activity of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> essential oils (EOs) an...
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MDPI AG
2022-09-01
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| Series: | Microbiology Research |
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| Online Access: | https://www.mdpi.com/2036-7481/13/3/48 |
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| author | Gregoria Mitropoulou Haido Bardouki Manolis Vamvakias Panayiotis Panas Panagiotis Paraskevas Yiannis Kourkoutas |
| author_facet | Gregoria Mitropoulou Haido Bardouki Manolis Vamvakias Panayiotis Panas Panagiotis Paraskevas Yiannis Kourkoutas |
| author_sort | Gregoria Mitropoulou |
| collection | DOAJ |
| description | Nowadays, the use of antimicrobial natural agents as alternative food preservatives represents an intriguing case. The purpose of this study was to investigate possible antimicrobial activity of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> essential oils (EOs) and to evaluate their commercial potential in the food industry. The main constituents identified by GC/MS in <i>Pistacia lentiscus</i> EO were a-pinene (67.7%), myrcene (18.8%), and β-pinene (3.0%), whereas limonene (93.8%) and myrcene (2.7%) were the dominant compounds in <i>Fortunella</i> <i>margarita</i> EO. The antimicrobial properties were initially assayed and the minimum inhibitory, non-inhibitory, and minimum lethal concentration values against the <i>Escherichia</i><i>coli</i>, <i>Listeria monocytogenes</i>, <i>Pseudomonas fragi</i>, <i>Aspergillus niger</i>, and <i>Saccharomyces cerevisiae</i> were determined using a previously published model, combining absorbance measurements with the common dilution method and non-linear regression analysis to fit the data. Their efficiency was further validated in ice cream containing 0.2% (<i>w</i>/<i>w</i>) <i>Pistacia lentiscus</i>, 0.006% (<i>w</i>/<i>w</i>) <i>Fortunella margarita</i> EOs and 2% (<i>w</i>/<i>w</i>) aqueous residue of <i>F. margarita</i> EO deliberately inoculated with 4 logcfu/g <i>Escherichia</i><i>coli</i>, <i>Listeria monocytogenes</i> or <i>Pseudomonas fragi</i>, separately. Similarly, the activity of the oils was monitored in fruit juice (lemon, apple, and blackcurrant) containing 0.2% (<i>w</i>/<i>w</i>) <i>Pistacia lentiscus</i>, 0.006% (<i>w</i>/<i>w</i>) <i>Fortunella margarita</i> EOs and 2% (<i>w</i>/<i>w</i>) aqueous residue of <i>F. margarita</i> EO deliberately spiked with 100 spores/mL of <i>Aspergillus niger</i> or 4 logcfu/mL of <i>Saccharomyces cerevisiae</i>, separately. The results showed that microbial viable counts in the supplemented products ranged at significantly lower levels compared to the control samples during storage. Overall, the data indicated that both EOs constitute effective antimicrobial sources with many potent applications in the food industry. |
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| last_indexed | 2024-03-09T23:09:06Z |
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| spelling | doaj.art-b7f57779312e410db0a891f9baf9cc422023-11-23T17:48:20ZengMDPI AGMicrobiology Research2036-74812022-09-0113366768010.3390/microbiolres13030048Assessment of Antimicrobial Efficiency of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> Essential Oils against Spoilage and Pathogenic Microbes in Ice Cream and Fruit JuicesGregoria Mitropoulou0Haido Bardouki1Manolis Vamvakias2Panayiotis Panas3Panagiotis Paraskevas4Yiannis Kourkoutas5Laboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology and Genetics, Democritus University of Thrace, GR-68100 Alexandroupolis, GreeceVioryl, Chemical and Agricultural Industry, Research S.A., 28th km Athens-Lamia National R.D., P.O. Box 149, GR-19014 Afidnes, GreeceVioryl, Chemical and Agricultural Industry, Research S.A., 28th km Athens-Lamia National R.D., P.O. Box 149, GR-19014 Afidnes, GreeceQLCon, N.E.O. Patron Athinon 57, GR-25018 Patras, GreeceEMFI S.A., 88 Iera Odos St, GR-10447 Athens, GreeceLaboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology and Genetics, Democritus University of Thrace, GR-68100 Alexandroupolis, GreeceNowadays, the use of antimicrobial natural agents as alternative food preservatives represents an intriguing case. The purpose of this study was to investigate possible antimicrobial activity of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> essential oils (EOs) and to evaluate their commercial potential in the food industry. The main constituents identified by GC/MS in <i>Pistacia lentiscus</i> EO were a-pinene (67.7%), myrcene (18.8%), and β-pinene (3.0%), whereas limonene (93.8%) and myrcene (2.7%) were the dominant compounds in <i>Fortunella</i> <i>margarita</i> EO. The antimicrobial properties were initially assayed and the minimum inhibitory, non-inhibitory, and minimum lethal concentration values against the <i>Escherichia</i><i>coli</i>, <i>Listeria monocytogenes</i>, <i>Pseudomonas fragi</i>, <i>Aspergillus niger</i>, and <i>Saccharomyces cerevisiae</i> were determined using a previously published model, combining absorbance measurements with the common dilution method and non-linear regression analysis to fit the data. Their efficiency was further validated in ice cream containing 0.2% (<i>w</i>/<i>w</i>) <i>Pistacia lentiscus</i>, 0.006% (<i>w</i>/<i>w</i>) <i>Fortunella margarita</i> EOs and 2% (<i>w</i>/<i>w</i>) aqueous residue of <i>F. margarita</i> EO deliberately inoculated with 4 logcfu/g <i>Escherichia</i><i>coli</i>, <i>Listeria monocytogenes</i> or <i>Pseudomonas fragi</i>, separately. Similarly, the activity of the oils was monitored in fruit juice (lemon, apple, and blackcurrant) containing 0.2% (<i>w</i>/<i>w</i>) <i>Pistacia lentiscus</i>, 0.006% (<i>w</i>/<i>w</i>) <i>Fortunella margarita</i> EOs and 2% (<i>w</i>/<i>w</i>) aqueous residue of <i>F. margarita</i> EO deliberately spiked with 100 spores/mL of <i>Aspergillus niger</i> or 4 logcfu/mL of <i>Saccharomyces cerevisiae</i>, separately. The results showed that microbial viable counts in the supplemented products ranged at significantly lower levels compared to the control samples during storage. Overall, the data indicated that both EOs constitute effective antimicrobial sources with many potent applications in the food industry.https://www.mdpi.com/2036-7481/13/3/48essential oilsspoilagefoodborne pathogensice creamfruit juices<i>Pistacia lentiscus</i> |
| spellingShingle | Gregoria Mitropoulou Haido Bardouki Manolis Vamvakias Panayiotis Panas Panagiotis Paraskevas Yiannis Kourkoutas Assessment of Antimicrobial Efficiency of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> Essential Oils against Spoilage and Pathogenic Microbes in Ice Cream and Fruit Juices Microbiology Research essential oils spoilage foodborne pathogens ice cream fruit juices <i>Pistacia lentiscus</i> |
| title | Assessment of Antimicrobial Efficiency of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> Essential Oils against Spoilage and Pathogenic Microbes in Ice Cream and Fruit Juices |
| title_full | Assessment of Antimicrobial Efficiency of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> Essential Oils against Spoilage and Pathogenic Microbes in Ice Cream and Fruit Juices |
| title_fullStr | Assessment of Antimicrobial Efficiency of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> Essential Oils against Spoilage and Pathogenic Microbes in Ice Cream and Fruit Juices |
| title_full_unstemmed | Assessment of Antimicrobial Efficiency of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> Essential Oils against Spoilage and Pathogenic Microbes in Ice Cream and Fruit Juices |
| title_short | Assessment of Antimicrobial Efficiency of <i>Pistacia lentiscus</i> and <i>Fortunella margarita</i> Essential Oils against Spoilage and Pathogenic Microbes in Ice Cream and Fruit Juices |
| title_sort | assessment of antimicrobial efficiency of i pistacia lentiscus i and i fortunella margarita i essential oils against spoilage and pathogenic microbes in ice cream and fruit juices |
| topic | essential oils spoilage foodborne pathogens ice cream fruit juices <i>Pistacia lentiscus</i> |
| url | https://www.mdpi.com/2036-7481/13/3/48 |
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