A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin
A comparative analysis of the structure and biological properties of silk fibroin constructions was performed. Three groups of constructions were obtained: films obtained by casting an aqueous solution of silk fibroin and electrospun microfibrous scaffolds based on silk fibroin, with the addition of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/10/1561 |
| _version_ | 1797513434708836352 |
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| author | Liubov Safonova Maria Bobrova Anton Efimov Alexey Lyundup Olga Agapova Igor Agapov |
| author_facet | Liubov Safonova Maria Bobrova Anton Efimov Alexey Lyundup Olga Agapova Igor Agapov |
| author_sort | Liubov Safonova |
| collection | DOAJ |
| description | A comparative analysis of the structure and biological properties of silk fibroin constructions was performed. Three groups of constructions were obtained: films obtained by casting an aqueous solution of silk fibroin and electrospun microfibrous scaffolds based on silk fibroin, with the addition of 30% gelatin per total protein weight. The internal structures of the films and single fibers of the microfibrous scaffolds consisted of densely packed globule structures; the surface area to volume ratios and volume porosities of the microfibrous scaffolds were calculated. All constructions were non-toxic for cells and provide high levels of adhesion and proliferation. The high regenerative potential of the constructions was demonstrated in a rat full-thickness skin wound healing model. The constructions accelerated healing by an average of 15 days and can be considered to be promising constructions for various tasks of tissue engineering and regenerative medicine. |
| first_indexed | 2024-03-10T06:17:32Z |
| format | Article |
| id | doaj.art-b7fa3cffca084f5887c9509796ef16eb |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T06:17:32Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-b7fa3cffca084f5887c9509796ef16eb2023-11-22T19:37:34ZengMDPI AGPharmaceutics1999-49232021-09-011310156110.3390/pharmaceutics13101561A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk FibroinLiubov Safonova0Maria Bobrova1Anton Efimov2Alexey Lyundup3Olga Agapova4Igor Agapov5Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya ul. 1, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya ul. 1, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya ul. 1, 123182 Moscow, RussiaEducational Resource Center for Cellular Technologies, Peoples’ Friendship University of Russia, Miklukho-Maklaya str. 6, 117198 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya ul. 1, 123182 Moscow, RussiaAcademician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Schukinskaya ul. 1, 123182 Moscow, RussiaA comparative analysis of the structure and biological properties of silk fibroin constructions was performed. Three groups of constructions were obtained: films obtained by casting an aqueous solution of silk fibroin and electrospun microfibrous scaffolds based on silk fibroin, with the addition of 30% gelatin per total protein weight. The internal structures of the films and single fibers of the microfibrous scaffolds consisted of densely packed globule structures; the surface area to volume ratios and volume porosities of the microfibrous scaffolds were calculated. All constructions were non-toxic for cells and provide high levels of adhesion and proliferation. The high regenerative potential of the constructions was demonstrated in a rat full-thickness skin wound healing model. The constructions accelerated healing by an average of 15 days and can be considered to be promising constructions for various tasks of tissue engineering and regenerative medicine.https://www.mdpi.com/1999-4923/13/10/1561silk fibroin filmsbiocompatible polymerselectrospinningscanning probe nanotomographyfull-thickness skin wound |
| spellingShingle | Liubov Safonova Maria Bobrova Anton Efimov Alexey Lyundup Olga Agapova Igor Agapov A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin Pharmaceutics silk fibroin films biocompatible polymers electrospinning scanning probe nanotomography full-thickness skin wound |
| title | A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin |
| title_full | A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin |
| title_fullStr | A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin |
| title_full_unstemmed | A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin |
| title_short | A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin |
| title_sort | comparative analysis of the structure and biological properties of films and microfibrous scaffolds based on silk fibroin |
| topic | silk fibroin films biocompatible polymers electrospinning scanning probe nanotomography full-thickness skin wound |
| url | https://www.mdpi.com/1999-4923/13/10/1561 |
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