DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha
Abstract Mitochondria increase in number by the fission of existing mitochondria. Mitochondrial fission is needed to provide mitochondria to daughter cells during cell division. In Arabidopsis thaliana, four kinds of genes have been reported to be involved in mitochondrial fission. Two of them, DRP3...
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-04886-0 |
| _version_ | 1819127270977044480 |
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| author | Nagisa Nagaoka Akihiro Yamashita Rina Kurisu Yuta Watari Fumiko Ishizuna Nobuhissro Tsutsumi Kimitsune Ishizaki Takayuki Kohchi Shin-ichi Arimura |
| author_facet | Nagisa Nagaoka Akihiro Yamashita Rina Kurisu Yuta Watari Fumiko Ishizuna Nobuhissro Tsutsumi Kimitsune Ishizaki Takayuki Kohchi Shin-ichi Arimura |
| author_sort | Nagisa Nagaoka |
| collection | DOAJ |
| description | Abstract Mitochondria increase in number by the fission of existing mitochondria. Mitochondrial fission is needed to provide mitochondria to daughter cells during cell division. In Arabidopsis thaliana, four kinds of genes have been reported to be involved in mitochondrial fission. Two of them, DRP3 (dynamin-related protein3) and FIS1 (FISSION1), are well conserved in eukaryotes. The other two are plant-specific ELM1 (elongated mitochondria1) and PMD (peroxisomal and mitochondrial division). To better understand the commonality and diversity of mitochondrial fission factors in land plants, we examined mitochondrial fission-related genes in a liverwort, Marchantia polymorpha. As a bryophyte, M. polymorpha has features distinct from those of the other land plant lineages. We found that M. polymorpha has single copies of homologues for DRP3, FIS1 and ELM1, but does not appear to have a homologue of PMD. Citrine-fusion proteins with MpDRP3, MpFIS1 and MpELM1 were localized to mitochondria in M. polymorpha. MpDRP3- and MpELM1-defective mutants grew slowly and had networked mitochondria, indicating that mitochondrial fission was blocked in the mutants, as expected. However, knockout of MpFIS1 did not affect growth or mitochondrial morphology. These results suggest that MpDRP3 and MpELM1 but neither MpFIS1 nor PMD are needed for mitochondrial fission in M. polymorpha. |
| first_indexed | 2024-12-22T08:09:16Z |
| format | Article |
| id | doaj.art-b7faa266b0d14e76bc9747a5ea0f3338 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T08:09:16Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b7faa266b0d14e76bc9747a5ea0f33382022-12-21T18:33:04ZengNature PortfolioScientific Reports2045-23222017-07-017111010.1038/s41598-017-04886-0DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorphaNagisa Nagaoka0Akihiro Yamashita1Rina Kurisu2Yuta Watari3Fumiko Ishizuna4Nobuhissro Tsutsumi5Kimitsune Ishizaki6Takayuki Kohchi7Shin-ichi Arimura8Graduate School of Agricultural and Life Sciences, The University of TokyoGraduate School of Agricultural and Life Sciences, The University of TokyoGraduate School of Agricultural and Life Sciences, The University of TokyoGraduate School of Agricultural and Life Sciences, The University of TokyoGraduate School of Agricultural and Life Sciences, The University of TokyoGraduate School of Agricultural and Life Sciences, The University of TokyoGraduate School of Science, Kobe UniversityGraduate School of Biostudies, Kyoto UniversityGraduate School of Agricultural and Life Sciences, The University of TokyoAbstract Mitochondria increase in number by the fission of existing mitochondria. Mitochondrial fission is needed to provide mitochondria to daughter cells during cell division. In Arabidopsis thaliana, four kinds of genes have been reported to be involved in mitochondrial fission. Two of them, DRP3 (dynamin-related protein3) and FIS1 (FISSION1), are well conserved in eukaryotes. The other two are plant-specific ELM1 (elongated mitochondria1) and PMD (peroxisomal and mitochondrial division). To better understand the commonality and diversity of mitochondrial fission factors in land plants, we examined mitochondrial fission-related genes in a liverwort, Marchantia polymorpha. As a bryophyte, M. polymorpha has features distinct from those of the other land plant lineages. We found that M. polymorpha has single copies of homologues for DRP3, FIS1 and ELM1, but does not appear to have a homologue of PMD. Citrine-fusion proteins with MpDRP3, MpFIS1 and MpELM1 were localized to mitochondria in M. polymorpha. MpDRP3- and MpELM1-defective mutants grew slowly and had networked mitochondria, indicating that mitochondrial fission was blocked in the mutants, as expected. However, knockout of MpFIS1 did not affect growth or mitochondrial morphology. These results suggest that MpDRP3 and MpELM1 but neither MpFIS1 nor PMD are needed for mitochondrial fission in M. polymorpha.https://doi.org/10.1038/s41598-017-04886-0 |
| spellingShingle | Nagisa Nagaoka Akihiro Yamashita Rina Kurisu Yuta Watari Fumiko Ishizuna Nobuhissro Tsutsumi Kimitsune Ishizaki Takayuki Kohchi Shin-ichi Arimura DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha Scientific Reports |
| title | DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha |
| title_full | DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha |
| title_fullStr | DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha |
| title_full_unstemmed | DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha |
| title_short | DRP3 and ELM1 are required for mitochondrial fission in the liverwort Marchantia polymorpha |
| title_sort | drp3 and elm1 are required for mitochondrial fission in the liverwort marchantia polymorpha |
| url | https://doi.org/10.1038/s41598-017-04886-0 |
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