Scanning electron microscopy and Fourier transmission analysis of polyhydroxyalkanoates isolated from bacteria species from abattoir in Ota, Nigeria

This study reports production of polyhydroxyalkanoates (PHA) from microbial species obtained from abattoir by altering different carbon source (acetate and molasses) in minimal salt medium. The isolates were characterized by understanding their physiological responses to biochemical tests, optical m...

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Main Authors: Obinna C. Nwinyi, Tolulope A. Owolabi
Format: Article
Language:English
Published: Elsevier 2019-07-01
Series:Journal of King Saud University: Science
Online Access:http://www.sciencedirect.com/science/article/pii/S1018364717301398
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author Obinna C. Nwinyi
Tolulope A. Owolabi
author_facet Obinna C. Nwinyi
Tolulope A. Owolabi
author_sort Obinna C. Nwinyi
collection DOAJ
description This study reports production of polyhydroxyalkanoates (PHA) from microbial species obtained from abattoir by altering different carbon source (acetate and molasses) in minimal salt medium. The isolates were characterized by understanding their physiological responses to biochemical tests, optical microscopy examination and comparison to standard reference organisms. They were presumably species of Lactobacillus, Bacillus, Corynebacterium, Arthrobacter and Rhodococcus. The PHA products from these isolates were confirmed to be of plastic origin using the Fourier transmission analysis and the microstructure analysis elucidated by the scanning electron microscopy coupled with electron dispersive spectra. The sourcing of this resource (PHA) from bacteria species domiciled in the high organic environment could provide amazing metabolic machinery that could create a cost effective means for PHA production. Keywords: Abbatoir, Polyhydroxyalkanoates, Bacteria species, Molasses, Acetic acid
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spelling doaj.art-b7fbd1e933e0448e8719e583fc57f19e2022-12-21T17:32:55ZengElsevierJournal of King Saud University: Science1018-36472019-07-01313285298Scanning electron microscopy and Fourier transmission analysis of polyhydroxyalkanoates isolated from bacteria species from abattoir in Ota, NigeriaObinna C. Nwinyi0Tolulope A. Owolabi1Corresponding author.; Department of Biological Sciences, School of Natural and Applied Sciences, College of Science and Technology, Covenant University, Km 10 Idiroko Road, Canaan Land, PMB 1023 Ota, Ogun State, NigeriaDepartment of Biological Sciences, School of Natural and Applied Sciences, College of Science and Technology, Covenant University, Km 10 Idiroko Road, Canaan Land, PMB 1023 Ota, Ogun State, NigeriaThis study reports production of polyhydroxyalkanoates (PHA) from microbial species obtained from abattoir by altering different carbon source (acetate and molasses) in minimal salt medium. The isolates were characterized by understanding their physiological responses to biochemical tests, optical microscopy examination and comparison to standard reference organisms. They were presumably species of Lactobacillus, Bacillus, Corynebacterium, Arthrobacter and Rhodococcus. The PHA products from these isolates were confirmed to be of plastic origin using the Fourier transmission analysis and the microstructure analysis elucidated by the scanning electron microscopy coupled with electron dispersive spectra. The sourcing of this resource (PHA) from bacteria species domiciled in the high organic environment could provide amazing metabolic machinery that could create a cost effective means for PHA production. Keywords: Abbatoir, Polyhydroxyalkanoates, Bacteria species, Molasses, Acetic acidhttp://www.sciencedirect.com/science/article/pii/S1018364717301398
spellingShingle Obinna C. Nwinyi
Tolulope A. Owolabi
Scanning electron microscopy and Fourier transmission analysis of polyhydroxyalkanoates isolated from bacteria species from abattoir in Ota, Nigeria
Journal of King Saud University: Science
title Scanning electron microscopy and Fourier transmission analysis of polyhydroxyalkanoates isolated from bacteria species from abattoir in Ota, Nigeria
title_full Scanning electron microscopy and Fourier transmission analysis of polyhydroxyalkanoates isolated from bacteria species from abattoir in Ota, Nigeria
title_fullStr Scanning electron microscopy and Fourier transmission analysis of polyhydroxyalkanoates isolated from bacteria species from abattoir in Ota, Nigeria
title_full_unstemmed Scanning electron microscopy and Fourier transmission analysis of polyhydroxyalkanoates isolated from bacteria species from abattoir in Ota, Nigeria
title_short Scanning electron microscopy and Fourier transmission analysis of polyhydroxyalkanoates isolated from bacteria species from abattoir in Ota, Nigeria
title_sort scanning electron microscopy and fourier transmission analysis of polyhydroxyalkanoates isolated from bacteria species from abattoir in ota nigeria
url http://www.sciencedirect.com/science/article/pii/S1018364717301398
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AT tolulopeaowolabi scanningelectronmicroscopyandfouriertransmissionanalysisofpolyhydroxyalkanoatesisolatedfrombacteriaspeciesfromabattoirinotanigeria