Coordinated and Independent Roles for MLH Subunits in DNA Repair
The MutL family of DNA mismatch repair proteins (MMR) acts to maintain genomic integrity in somatic and meiotic cells. In baker’s yeast, the MutL homolog (MLH) MMR proteins form three heterodimeric complexes, MLH1-PMS1, MLH1-MLH2, and MLH1-MLH3. The recent discovery of human PMS2 (homolog of baker’s...
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MDPI AG
2021-04-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/4/948 |
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| author | Gianno Pannafino Eric Alani |
| author_facet | Gianno Pannafino Eric Alani |
| author_sort | Gianno Pannafino |
| collection | DOAJ |
| description | The MutL family of DNA mismatch repair proteins (MMR) acts to maintain genomic integrity in somatic and meiotic cells. In baker’s yeast, the MutL homolog (MLH) MMR proteins form three heterodimeric complexes, MLH1-PMS1, MLH1-MLH2, and MLH1-MLH3. The recent discovery of human PMS2 (homolog of baker’s yeast PMS1) and MLH3 acting independently of human MLH1 in the repair of somatic double-strand breaks questions the assumption that MLH1 is an obligate subunit for MLH function. Here we provide a summary of the canonical roles for MLH factors in DNA genomic maintenance and in meiotic crossover. We then present the phenotypes of cells lacking specific MLH subunits, particularly in meiotic recombination, and based on this analysis, propose a model for an independent early role for MLH3 in meiosis to promote the accurate segregation of homologous chromosomes in the meiosis I division. |
| first_indexed | 2024-03-10T12:09:55Z |
| format | Article |
| id | doaj.art-b7fcd1e83f814b738c9624513ddfa93b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T12:09:55Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-b7fcd1e83f814b738c9624513ddfa93b2023-11-21T16:16:53ZengMDPI AGCells2073-44092021-04-0110494810.3390/cells10040948Coordinated and Independent Roles for MLH Subunits in DNA RepairGianno Pannafino0Eric Alani1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703, USADepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703, USAThe MutL family of DNA mismatch repair proteins (MMR) acts to maintain genomic integrity in somatic and meiotic cells. In baker’s yeast, the MutL homolog (MLH) MMR proteins form three heterodimeric complexes, MLH1-PMS1, MLH1-MLH2, and MLH1-MLH3. The recent discovery of human PMS2 (homolog of baker’s yeast PMS1) and MLH3 acting independently of human MLH1 in the repair of somatic double-strand breaks questions the assumption that MLH1 is an obligate subunit for MLH function. Here we provide a summary of the canonical roles for MLH factors in DNA genomic maintenance and in meiotic crossover. We then present the phenotypes of cells lacking specific MLH subunits, particularly in meiotic recombination, and based on this analysis, propose a model for an independent early role for MLH3 in meiosis to promote the accurate segregation of homologous chromosomes in the meiosis I division.https://www.mdpi.com/2073-4409/10/4/948MutL homologsMLHDNA mismatch repairmeiosisHolliday junction resolutionhomologous recombination |
| spellingShingle | Gianno Pannafino Eric Alani Coordinated and Independent Roles for MLH Subunits in DNA Repair Cells MutL homologs MLH DNA mismatch repair meiosis Holliday junction resolution homologous recombination |
| title | Coordinated and Independent Roles for MLH Subunits in DNA Repair |
| title_full | Coordinated and Independent Roles for MLH Subunits in DNA Repair |
| title_fullStr | Coordinated and Independent Roles for MLH Subunits in DNA Repair |
| title_full_unstemmed | Coordinated and Independent Roles for MLH Subunits in DNA Repair |
| title_short | Coordinated and Independent Roles for MLH Subunits in DNA Repair |
| title_sort | coordinated and independent roles for mlh subunits in dna repair |
| topic | MutL homologs MLH DNA mismatch repair meiosis Holliday junction resolution homologous recombination |
| url | https://www.mdpi.com/2073-4409/10/4/948 |
| work_keys_str_mv | AT giannopannafino coordinatedandindependentrolesformlhsubunitsindnarepair AT ericalani coordinatedandindependentrolesformlhsubunitsindnarepair |