Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut
Aim: To determine whether selected gut bacteria of crocodile exhibit antibacterial properties. Materials & methods: Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and m...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Future Science Ltd
2023-06-01
|
| Series: | Future Science OA |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/fsoa-2023-0030 |
| _version_ | 1797832924834299904 |
|---|---|
| author | Ruqaiyyah Siddiqui Noor Akbar Nelson Cruz Soares Hamza Mohammad Al-Hroub Mohammad Harb Semreen Sutherland K Maciver Naveed Ahmed Khan |
| author_facet | Ruqaiyyah Siddiqui Noor Akbar Nelson Cruz Soares Hamza Mohammad Al-Hroub Mohammad Harb Semreen Sutherland K Maciver Naveed Ahmed Khan |
| author_sort | Ruqaiyyah Siddiqui |
| collection | DOAJ |
| description | Aim: To determine whether selected gut bacteria of crocodile exhibit antibacterial properties. Materials & methods: Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and metabolites were analyzed using liquid chromatography-mass spectrometry. Results & conclusion: Antibacterial assays revealed that conditioned media showed potent effects against pathogenic Gram-positive and Gram-negative bacteria. LC–MS revealed identity of 210 metabolites. The abundant metabolites were, N-Acetyl-L-tyrosine, Acetaminophen, Trans-Ferulic acid, N, N-Dimethylformamide, Pyrocatechol, Cyclohexanone, Diphenhydramine, Melatonin, Gamma–terpinene, Cysteamine, 3-phenoxypropionic acid, Indole-3-carbinol, Benzaldehyde, Benzocaine, 2-Aminobenzoic acid, 3-Methylindole. These findings suggest that crocodile gut bacteria are potential source of novel bioactive molecules that can be utilized as pre/post/antibiotics for the benefit of human health. |
| first_indexed | 2024-04-09T14:15:30Z |
| format | Article |
| id | doaj.art-b80021e8a17b4ec98ae63d1ebfd7542e |
| institution | Directory Open Access Journal |
| issn | 2056-5623 |
| language | English |
| last_indexed | 2024-04-09T14:15:30Z |
| publishDate | 2023-06-01 |
| publisher | Future Science Ltd |
| record_format | Article |
| series | Future Science OA |
| spelling | doaj.art-b80021e8a17b4ec98ae63d1ebfd7542e2023-05-05T11:17:59ZengFuture Science LtdFuture Science OA2056-56232023-06-019510.2144/fsoa-2023-0030Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gutRuqaiyyah Siddiqui0Noor Akbar1Nelson Cruz Soares2Hamza Mohammad Al-Hroub3Mohammad Harb Semreen4Sutherland K Maciver5Naveed Ahmed Khan61College of Arts & Sciences, American University of Sharjah, University City, Sharjah, 26666, United Arab Emirates3Department of Clinical Sciences, College of Medicine, University of Sharjah, Sharjah, 27272, United Arab Emirates4Department of Medicinal Chemistry, College of Pharmacy, University of Sharjah, Sharjah, 27272, United Arab Emirates5Sharjah Institute for Medical Research, University of Sharjah, Sharjah, 27272, United Arab Emirates4Department of Medicinal Chemistry, College of Pharmacy, University of Sharjah, Sharjah, 27272, United Arab Emirates6Centre for Discovery Brain Sciences, Edinburgh Medical School: Biomedical Sciences, University of Edinburgh, Edinburgh, UK2Department of Medical Biology, Faculty of Medicine, Istinye University, Istanbul, 34010, TurkeyAim: To determine whether selected gut bacteria of crocodile exhibit antibacterial properties. Materials & methods: Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and metabolites were analyzed using liquid chromatography-mass spectrometry. Results & conclusion: Antibacterial assays revealed that conditioned media showed potent effects against pathogenic Gram-positive and Gram-negative bacteria. LC–MS revealed identity of 210 metabolites. The abundant metabolites were, N-Acetyl-L-tyrosine, Acetaminophen, Trans-Ferulic acid, N, N-Dimethylformamide, Pyrocatechol, Cyclohexanone, Diphenhydramine, Melatonin, Gamma–terpinene, Cysteamine, 3-phenoxypropionic acid, Indole-3-carbinol, Benzaldehyde, Benzocaine, 2-Aminobenzoic acid, 3-Methylindole. These findings suggest that crocodile gut bacteria are potential source of novel bioactive molecules that can be utilized as pre/post/antibiotics for the benefit of human health.https://www.future-science.com/doi/10.2144/fsoa-2023-0030antibiotic resistancegut microbiotametabolomics |
| spellingShingle | Ruqaiyyah Siddiqui Noor Akbar Nelson Cruz Soares Hamza Mohammad Al-Hroub Mohammad Harb Semreen Sutherland K Maciver Naveed Ahmed Khan Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut Future Science OA antibiotic resistance gut microbiota metabolomics |
| title | Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut |
| title_full | Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut |
| title_fullStr | Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut |
| title_full_unstemmed | Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut |
| title_short | Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut |
| title_sort | mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut |
| topic | antibiotic resistance gut microbiota metabolomics |
| url | https://www.future-science.com/doi/10.2144/fsoa-2023-0030 |
| work_keys_str_mv | AT ruqaiyyahsiddiqui massspectrometricanalysisofbioactiveconditionedmediaofbacteriaisolatedfromreptiliangut AT noorakbar massspectrometricanalysisofbioactiveconditionedmediaofbacteriaisolatedfromreptiliangut AT nelsoncruzsoares massspectrometricanalysisofbioactiveconditionedmediaofbacteriaisolatedfromreptiliangut AT hamzamohammadalhroub massspectrometricanalysisofbioactiveconditionedmediaofbacteriaisolatedfromreptiliangut AT mohammadharbsemreen massspectrometricanalysisofbioactiveconditionedmediaofbacteriaisolatedfromreptiliangut AT sutherlandkmaciver massspectrometricanalysisofbioactiveconditionedmediaofbacteriaisolatedfromreptiliangut AT naveedahmedkhan massspectrometricanalysisofbioactiveconditionedmediaofbacteriaisolatedfromreptiliangut |