Use of natural substances for boar semen decontamination
The aim of this study was to investigate the antibacterial activity and toxicity for sperm cells of the natural substances gallic acid, methyl gallate, ethyl gallate, propyl gallate, octyl gallate, thymol, carvacrol and eugenol. The antibacterial activity of these natural substances and selected com...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-05-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201505-0002_use-of-natural-substances-for-boar-semen-decontamination.php |
| _version_ | 1797897541002461184 |
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| author | J. Mazurova R. Kukla M. Rozkot A. Lustykova E. Slehova R. Sleha J. Lipensky L. Opletal |
| author_facet | J. Mazurova R. Kukla M. Rozkot A. Lustykova E. Slehova R. Sleha J. Lipensky L. Opletal |
| author_sort | J. Mazurova |
| collection | DOAJ |
| description | The aim of this study was to investigate the antibacterial activity and toxicity for sperm cells of the natural substances gallic acid, methyl gallate, ethyl gallate, propyl gallate, octyl gallate, thymol, carvacrol and eugenol. The antibacterial activity of these natural substances and selected combinations of them against bacterial strains isolated from boar ejaculates was determined using the microdilution and macrodilution method in Mueller-Hinton broth. The most effective natural substances against Gram-negative and Gram-positive bacteria included in our study were thymol and carvacrol with minimum inhibitory concentration (MIC) values in the range of 300-600 µg/ml. Gallic acid exhibited the best antibacterial activity against Pseudomonas aeruginosa strains (MIC values of 300-2400 µg/ml), whereas the ranges of MIC values against Escherichia coli, Enterococcus faecalis and Staphylococcus sp. strains were higher. Octyl gallate exhibited stronger antibacterial activity against staphylococci and enterococci (MIC values of 18.8-75 µg/ml) than against Escherichia coli and Pseudomonas aeruginosa strains with MIC values in the ranges of 300-600 µg/mland 1200-2400 µg/ml, respectively. Thymol combined with carvacrol was the most effective combination against enterococci (MIC values of 75-300 : 150 thymol : carvacrol) and Pseudomonas aeruginosa (MIC values of 75-300 : 300 thymol : carvacrol), bacteria which are known to be frequently resistant to antimicrobials. Similar results were determined for the combination of carvacrol and eugenol against staphylococci and enterococci. The results of the combinations revealed more of an additive rather than a synergistic effect. Thymol and carvacrol were the most effective natural substances against the bacteria included in this study, with a low toxicity for sperm cells compared to other substances, suggesting their possible use for boar semen decontamination. |
| first_indexed | 2024-04-10T07:59:58Z |
| format | Article |
| id | doaj.art-b8046e495a144964837c466b66d0863f |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:59:58Z |
| publishDate | 2015-05-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-b8046e495a144964837c466b66d0863f2023-02-23T03:50:24ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922015-05-0160523524710.17221/8175-VETMEDvet-201505-0002Use of natural substances for boar semen decontaminationJ. Mazurova0R. Kukla1M. Rozkot2A. Lustykova3E. Slehova4R. Sleha5J. Lipensky6L. Opletal7Faculty of Chemical-Technology, University of Pardubice, Czech RepublicFaculty of Chemical-Technology, University of Pardubice, Czech RepublicInstitute of Animal Science, Prague-Uhrineves, Workplace Kostelec nad Orlici, Czech RepublicInstitute of Animal Science, Prague-Uhrineves, Workplace Kostelec nad Orlici, Czech RepublicFaculty of Chemical-Technology, University of Pardubice, Czech RepublicFaculty of Chemical-Technology, University of Pardubice, Czech RepublicInstitute of Animal Science, Prague-Uhrineves, Workplace Kostelec nad Orlici, Czech RepublicFaculty of Pharmacy, Hradec Kralove, Charles University, Prague, Czech RepublicThe aim of this study was to investigate the antibacterial activity and toxicity for sperm cells of the natural substances gallic acid, methyl gallate, ethyl gallate, propyl gallate, octyl gallate, thymol, carvacrol and eugenol. The antibacterial activity of these natural substances and selected combinations of them against bacterial strains isolated from boar ejaculates was determined using the microdilution and macrodilution method in Mueller-Hinton broth. The most effective natural substances against Gram-negative and Gram-positive bacteria included in our study were thymol and carvacrol with minimum inhibitory concentration (MIC) values in the range of 300-600 µg/ml. Gallic acid exhibited the best antibacterial activity against Pseudomonas aeruginosa strains (MIC values of 300-2400 µg/ml), whereas the ranges of MIC values against Escherichia coli, Enterococcus faecalis and Staphylococcus sp. strains were higher. Octyl gallate exhibited stronger antibacterial activity against staphylococci and enterococci (MIC values of 18.8-75 µg/ml) than against Escherichia coli and Pseudomonas aeruginosa strains with MIC values in the ranges of 300-600 µg/mland 1200-2400 µg/ml, respectively. Thymol combined with carvacrol was the most effective combination against enterococci (MIC values of 75-300 : 150 thymol : carvacrol) and Pseudomonas aeruginosa (MIC values of 75-300 : 300 thymol : carvacrol), bacteria which are known to be frequently resistant to antimicrobials. Similar results were determined for the combination of carvacrol and eugenol against staphylococci and enterococci. The results of the combinations revealed more of an additive rather than a synergistic effect. Thymol and carvacrol were the most effective natural substances against the bacteria included in this study, with a low toxicity for sperm cells compared to other substances, suggesting their possible use for boar semen decontamination.https://vetmed.agriculturejournals.cz/artkey/vet-201505-0002_use-of-natural-substances-for-boar-semen-decontamination.phpthymolcarvacroleugenolgallic acidgallatesantibacterial activitybacteriaspermiotoxicity |
| spellingShingle | J. Mazurova R. Kukla M. Rozkot A. Lustykova E. Slehova R. Sleha J. Lipensky L. Opletal Use of natural substances for boar semen decontamination Veterinární Medicína thymol carvacrol eugenol gallic acid gallates antibacterial activity bacteria spermiotoxicity |
| title | Use of natural substances for boar semen decontamination |
| title_full | Use of natural substances for boar semen decontamination |
| title_fullStr | Use of natural substances for boar semen decontamination |
| title_full_unstemmed | Use of natural substances for boar semen decontamination |
| title_short | Use of natural substances for boar semen decontamination |
| title_sort | use of natural substances for boar semen decontamination |
| topic | thymol carvacrol eugenol gallic acid gallates antibacterial activity bacteria spermiotoxicity |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201505-0002_use-of-natural-substances-for-boar-semen-decontamination.php |
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